Neuronal Transcriptome of Aplysia: Neuronal Compartments and Circuitry

Leonid L. Moroz; John R. Edwards; Sathyanarayanan V. Puthanveettil; Andrea B. Kohn; Thomas Ha; Andreas Heyland; Bjarne Knudsen; Anuj Sahni; Fahong Yu; Li Liu; Sami Jezzini; Peter Lovell; William Iannucculli; Minchen Chen; Tuan Nguyen; Huitao Sheng; Regina Shaw; Sergey Kalachikov; Yuri V. Panchin; William Farmerie; James J. Russo; Jingyue Ju; Eric R. Kandel

doi:10.1016/j.cell.2006.09.052

Neuronal Transcriptome of Aplysia: Neuronal Compartments and Circuitry

Leonid L. Moroz, John R. Edwards, Sathyanarayanan V. Puthanveettil, Andrea B. Kohn, Thomas Ha, Andreas Heyland, Bjarne Knudsen, Anuj Sahni, Fahong Yu, Li Liu, Sami Jezzini, Peter Lovell, William Iannucculli, Minchen Chen, Tuan Nguyen, Huitao Sheng, Regina Shaw, Sergey Kalachikov, Yuri V. Panchin, William FarmerieJames J. Russo, Jingyue Ju, Eric R. Kandel

Research output: Contribution to journal › Article › peer-review

273 Scopus citations

Abstract

Molecular analyses of Aplysia, a well-established model organism for cellular and systems neural science, have been seriously handicapped by a lack of adequate genomic information. By sequencing cDNA libraries from the central nervous system (CNS), we have identified over 175,000 expressed sequence tags (ESTs), of which 19,814 are unique neuronal gene products and represent 50%-70% of the total Aplysia neuronal transcriptome. We have characterized the transcriptome at three levels: (1) the central nervous system, (2) the elementary components of a simple behavior: the gill-withdrawal reflex-by analyzing sensory, motor, and serotonergic modulatory neurons, and (3) processes of individual neurons. In addition to increasing the amount of available gene sequences of Aplysia by two orders of magnitude, this collection represents the largest database available for any member of the Lophotrochozoa and therefore provides additional insights into evolutionary strategies used by this highly successful diversified lineage, one of the three proposed superclades of bilateral animals.

Original language	English (US)
Pages (from-to)	1453-1467
Number of pages	15
Journal	Cell
Volume	127
Issue number	7
DOIs	https://doi.org/10.1016/j.cell.2006.09.052
State	Published - Dec 29 2006
Externally published	Yes

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology

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Moroz, L. L., Edwards, J. R., Puthanveettil, S. V., Kohn, A. B., Ha, T., Heyland, A., Knudsen, B., Sahni, A., Yu, F., Liu, L., Jezzini, S., Lovell, P., Iannucculli, W., Chen, M., Nguyen, T., Sheng, H., Shaw, R., Kalachikov, S., Panchin, Y. V., ... Kandel, E. R. (2006). Neuronal Transcriptome of Aplysia: Neuronal Compartments and Circuitry. Cell, 127(7), 1453-1467. https://doi.org/10.1016/j.cell.2006.09.052

Moroz, LL, Edwards, JR, Puthanveettil, SV, Kohn, AB, Ha, T, Heyland, A, Knudsen, B, Sahni, A, Yu, F, Liu, L, Jezzini, S, Lovell, P, Iannucculli, W, Chen, M, Nguyen, T, Sheng, H, Shaw, R, Kalachikov, S, Panchin, YV, Farmerie, W, Russo, JJ, Ju, J & Kandel, ER 2006, 'Neuronal Transcriptome of Aplysia: Neuronal Compartments and Circuitry', Cell, vol. 127, no. 7, pp. 1453-1467. https://doi.org/10.1016/j.cell.2006.09.052

@article{3d2fd62c24be4af4b05c43bc238f2b26,

title = "Neuronal Transcriptome of Aplysia: Neuronal Compartments and Circuitry",

abstract = "Molecular analyses of Aplysia, a well-established model organism for cellular and systems neural science, have been seriously handicapped by a lack of adequate genomic information. By sequencing cDNA libraries from the central nervous system (CNS), we have identified over 175,000 expressed sequence tags (ESTs), of which 19,814 are unique neuronal gene products and represent 50%-70% of the total Aplysia neuronal transcriptome. We have characterized the transcriptome at three levels: (1) the central nervous system, (2) the elementary components of a simple behavior: the gill-withdrawal reflex-by analyzing sensory, motor, and serotonergic modulatory neurons, and (3) processes of individual neurons. In addition to increasing the amount of available gene sequences of Aplysia by two orders of magnitude, this collection represents the largest database available for any member of the Lophotrochozoa and therefore provides additional insights into evolutionary strategies used by this highly successful diversified lineage, one of the three proposed superclades of bilateral animals.",

author = "Moroz, {Leonid L.} and Edwards, {John R.} and Puthanveettil, {Sathyanarayanan V.} and Kohn, {Andrea B.} and Thomas Ha and Andreas Heyland and Bjarne Knudsen and Anuj Sahni and Fahong Yu and Li Liu and Sami Jezzini and Peter Lovell and William Iannucculli and Minchen Chen and Tuan Nguyen and Huitao Sheng and Regina Shaw and Sergey Kalachikov and Panchin, {Yuri V.} and William Farmerie and Russo, {James J.} and Jingyue Ju and Kandel, {Eric R.}",

note = "Funding Information: We thank Tom Carew, Kelsey Martin, Kausik Si, Tom Jessel, Wayne Sossin, John Byrne, and Larry Zipursky for their comments on earlier versions of this paper. Our work is supported by HHMI, NSF, and National Institutes of Health Center of Excellence in Genomic Science Grants P50 HG002806 and R01 MH075026, NS39103, and in part by the McKnight Brain Research Foundations, UF Opportunity Funds, and RFBR- 05-04-48401. We also would like to thank Mrs. E. Bobkova, T. Brough, E. Meleshkevitch, J. Netherton, and Drs. M. Matz, N. Alieva, and R. Sadreyev for technical help and comments at earlier stages of this project. We thank MOgene (LC), and in particular Shaukat Rangwala, for the microarray analysis, and Drs. E. Koonin and Y. Wolf for KOGs discussions. Computational work for this project was supported by The AMDeC Bioinformatics Core Facility at the Columbia Genome Center, Columbia University, and the ICBR Core facility at the University of Florida. Currently, Y. Panchin is at Moscow State University, Moscow, Russia; and B. Knudsen is at CLC bio A/S Aarhus, Denmark. ",

year = "2006",

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day = "29",

doi = "10.1016/j.cell.2006.09.052",

language = "English (US)",

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pages = "1453--1467",

journal = "Cell",

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TY - JOUR

T1 - Neuronal Transcriptome of Aplysia

T2 - Neuronal Compartments and Circuitry

AU - Moroz, Leonid L.

AU - Edwards, John R.

AU - Puthanveettil, Sathyanarayanan V.

AU - Kohn, Andrea B.

AU - Ha, Thomas

AU - Heyland, Andreas

AU - Knudsen, Bjarne

AU - Sahni, Anuj

AU - Yu, Fahong

AU - Liu, Li

AU - Jezzini, Sami

AU - Lovell, Peter

AU - Iannucculli, William

AU - Chen, Minchen

AU - Nguyen, Tuan

AU - Sheng, Huitao

AU - Shaw, Regina

AU - Kalachikov, Sergey

AU - Panchin, Yuri V.

AU - Farmerie, William

AU - Russo, James J.

AU - Ju, Jingyue

AU - Kandel, Eric R.

N1 - Funding Information: We thank Tom Carew, Kelsey Martin, Kausik Si, Tom Jessel, Wayne Sossin, John Byrne, and Larry Zipursky for their comments on earlier versions of this paper. Our work is supported by HHMI, NSF, and National Institutes of Health Center of Excellence in Genomic Science Grants P50 HG002806 and R01 MH075026, NS39103, and in part by the McKnight Brain Research Foundations, UF Opportunity Funds, and RFBR- 05-04-48401. We also would like to thank Mrs. E. Bobkova, T. Brough, E. Meleshkevitch, J. Netherton, and Drs. M. Matz, N. Alieva, and R. Sadreyev for technical help and comments at earlier stages of this project. We thank MOgene (LC), and in particular Shaukat Rangwala, for the microarray analysis, and Drs. E. Koonin and Y. Wolf for KOGs discussions. Computational work for this project was supported by The AMDeC Bioinformatics Core Facility at the Columbia Genome Center, Columbia University, and the ICBR Core facility at the University of Florida. Currently, Y. Panchin is at Moscow State University, Moscow, Russia; and B. Knudsen is at CLC bio A/S Aarhus, Denmark.

PY - 2006/12/29

Y1 - 2006/12/29

N2 - Molecular analyses of Aplysia, a well-established model organism for cellular and systems neural science, have been seriously handicapped by a lack of adequate genomic information. By sequencing cDNA libraries from the central nervous system (CNS), we have identified over 175,000 expressed sequence tags (ESTs), of which 19,814 are unique neuronal gene products and represent 50%-70% of the total Aplysia neuronal transcriptome. We have characterized the transcriptome at three levels: (1) the central nervous system, (2) the elementary components of a simple behavior: the gill-withdrawal reflex-by analyzing sensory, motor, and serotonergic modulatory neurons, and (3) processes of individual neurons. In addition to increasing the amount of available gene sequences of Aplysia by two orders of magnitude, this collection represents the largest database available for any member of the Lophotrochozoa and therefore provides additional insights into evolutionary strategies used by this highly successful diversified lineage, one of the three proposed superclades of bilateral animals.

AB - Molecular analyses of Aplysia, a well-established model organism for cellular and systems neural science, have been seriously handicapped by a lack of adequate genomic information. By sequencing cDNA libraries from the central nervous system (CNS), we have identified over 175,000 expressed sequence tags (ESTs), of which 19,814 are unique neuronal gene products and represent 50%-70% of the total Aplysia neuronal transcriptome. We have characterized the transcriptome at three levels: (1) the central nervous system, (2) the elementary components of a simple behavior: the gill-withdrawal reflex-by analyzing sensory, motor, and serotonergic modulatory neurons, and (3) processes of individual neurons. In addition to increasing the amount of available gene sequences of Aplysia by two orders of magnitude, this collection represents the largest database available for any member of the Lophotrochozoa and therefore provides additional insights into evolutionary strategies used by this highly successful diversified lineage, one of the three proposed superclades of bilateral animals.

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ER -

Neuronal Transcriptome of Aplysia: Neuronal Compartments and Circuitry

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