Neuronal Transcriptome of Aplysia: Neuronal Compartments and Circuitry

Leonid L. Moroz, John R. Edwards, Sathyanarayanan V. Puthanveettil, Andrea B. Kohn, Thomas Ha, Andreas Heyland, Bjarne Knudsen, Anuj Sahni, Fahong Yu, Li Liu, Sami Jezzini, Peter Lovell, William Iannucculli, Minchen Chen, Tuan Nguyen, Huitao Sheng, Regina Shaw, Sergey Kalachikov, Yuri V. Panchin, William FarmerieJames J. Russo, Jingyue Ju, Eric R. Kandel

Research output: Contribution to journalArticle

225 Scopus citations

Abstract

Molecular analyses of Aplysia, a well-established model organism for cellular and systems neural science, have been seriously handicapped by a lack of adequate genomic information. By sequencing cDNA libraries from the central nervous system (CNS), we have identified over 175,000 expressed sequence tags (ESTs), of which 19,814 are unique neuronal gene products and represent 50%-70% of the total Aplysia neuronal transcriptome. We have characterized the transcriptome at three levels: (1) the central nervous system, (2) the elementary components of a simple behavior: the gill-withdrawal reflex-by analyzing sensory, motor, and serotonergic modulatory neurons, and (3) processes of individual neurons. In addition to increasing the amount of available gene sequences of Aplysia by two orders of magnitude, this collection represents the largest database available for any member of the Lophotrochozoa and therefore provides additional insights into evolutionary strategies used by this highly successful diversified lineage, one of the three proposed superclades of bilateral animals.

Original languageEnglish (US)
Pages (from-to)1453-1467
Number of pages15
JournalCell
Volume127
Issue number7
DOIs
StatePublished - Dec 29 2006

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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    Moroz, L. L., Edwards, J. R., Puthanveettil, S. V., Kohn, A. B., Ha, T., Heyland, A., Knudsen, B., Sahni, A., Yu, F., Liu, L., Jezzini, S., Lovell, P., Iannucculli, W., Chen, M., Nguyen, T., Sheng, H., Shaw, R., Kalachikov, S., Panchin, Y. V., ... Kandel, E. R. (2006). Neuronal Transcriptome of Aplysia: Neuronal Compartments and Circuitry. Cell, 127(7), 1453-1467. https://doi.org/10.1016/j.cell.2006.09.052