@article{fa04f64bf6bb423b8bc24521eee447bc,
title = "Neonatal immune-tolerance in mice does not prevent xenograft rejection",
abstract = "Assessing the efficacy of human stem cell transplantation in rodent models is complicated by the significant immune rejection that occurs. Two recent reports have shown conflicting results using neonatal tolerance to xenografts in rats. Here we extend this approach to mice and assess whether neonatal tolerance can prevent the rapid rejection of xenografts. In three strains of neonatal immune-intact mice, using two different brain transplant regimes and three independent stem cell types, we conclusively show that there is rapid rejection of the implanted cells. We also address specific challenges associated with the generation of humanized mouse models of disease.",
keywords = "Huntington's disease, Immune rejection, Neonatal immunity, Neonatal tolerance, Xenograft",
author = "Mattis, {Virginia B.} and Wakeman, {Dustin R.} and Colton Tom and Dodiya, {Hemraj B.} and Yeung, {Sylvia Y.} and Tran, {Andrew H.} and Ksenija Bernau and Loren Ornelas and Anais Sahabian and Jack Reidling and Dhruv Sareen and Thompson, {Leslie M.} and Kordower, {Jeffrey H.} and Svendsen, {Clive N.}",
note = "Funding Information: The authors would like to thank Laura McClung, Jessica Gould and Andrew T. Tran for the assistance with sectioning of animal brains and counting of transplanted cells, Dr. Patrick Aebischer for the generation of the LUC2 lentivirus, Dr. Soshana Svendsen for the critical review of the manuscript, Chad A. Zimprich (Promega) for the cloning of the LUC2 construct, and Dr. Shilpa Ramaswamy for the procedural assistance with stereotaxic transplantation. The authors would also like to thank and acknowledge Dr. Wafa Tawackoli and Xiaoyu Da at the Cedars-Sinai Medical Center Imaging Core for their assistance with in vivo luciferase imaging. STEMEZ hNP1 cells were a kind gift courtesy of Dr. Steven L. Stice at the University of Georgia. ENStem-A human neural progenitor cells expressing GFP were kindly provided by Mathew Blurton-Jones at UC Irvine. The plasmid expressing GFP driven by the CAGG promoter was kindly provided by Peter Andrews at the University of Sheffield. This work was funded by a Milton Wexler Post-Doctoral Fellowship from the Hereditary Disease Foundation (V.B.M.), NINDS 1R21NS074187-01 (J.H.K. & D.R.W.) and a CIRM Early Translation Award (L.M.T.) CIRM TR2-01841 .",
year = "2014",
month = apr,
doi = "10.1016/j.expneurol.2014.01.007",
language = "English (US)",
volume = "254",
pages = "90--98",
journal = "Neurodegeneration",
issn = "0014-4886",
publisher = "Academic Press Inc.",
}
