Neomycin resistance as a selectable marker in Methanococcus maripaludis

John L. Argyle, Debra Hansen, John A. Leigh

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Abstract

We cloned the aminoglycoside phosphotransferase genes APH3'I and APH3'II between the Methanococcus voltae methyl reductase promoter and terminator in a plasmid containing a fragment of Methanococcus maripaludis chromosomal DNA. The resulting plasmids encoding neomycin resistance transformed M. maripaludis at frequencies similar to those observed for pKAS102 encoding puromycin resistance. The antibiotic geneticin was not inhibitory to M. maripaludis.

Original languageEnglish (US)
Pages (from-to)4233-4237
Number of pages5
JournalApplied and Environmental Microbiology
Volume62
Issue number11
StatePublished - Nov 1 1996
Externally publishedYes

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ASJC Scopus subject areas

  • Environmental Science(all)
  • Biotechnology
  • Microbiology

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