Neomycin resistance as a selectable marker in Methanococcus maripaludis

John L. Argyle, Debra Hansen, John A. Leigh

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

We cloned the aminoglycoside phosphotransferase genes APH3'I and APH3'II between the Methanococcus voltae methyl reductase promoter and terminator in a plasmid containing a fragment of Methanococcus maripaludis chromosomal DNA. The resulting plasmids encoding neomycin resistance transformed M. maripaludis at frequencies similar to those observed for pKAS102 encoding puromycin resistance. The antibiotic geneticin was not inhibitory to M. maripaludis.

Original languageEnglish (US)
Pages (from-to)4233-4237
Number of pages5
JournalApplied and Environmental Microbiology
Volume62
Issue number11
StatePublished - Nov 1 1996
Externally publishedYes

Fingerprint

Methanococcus
Neomycin
neomycin
plasmid
Plasmids
Kanamycin Kinase
Puromycin
plasmids
geneticin
antibiotics
puromycin
aminoglycosides
Oxidoreductases
Anti-Bacterial Agents
DNA
gene
phosphotransferases (kinases)
promoter regions
Genes
Methanococcus maripaludis

ASJC Scopus subject areas

  • Environmental Science(all)
  • Biotechnology
  • Microbiology

Cite this

Neomycin resistance as a selectable marker in Methanococcus maripaludis. / Argyle, John L.; Hansen, Debra; Leigh, John A.

In: Applied and Environmental Microbiology, Vol. 62, No. 11, 01.11.1996, p. 4233-4237.

Research output: Contribution to journalArticle

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