Natural odor ligands for olfactory receptor neurons of the female mosquito Aedes aegypti: Use of gas chromatography-linked single sensillum recordings

Majid Ghaninia Tabarestani, Mattias Larsson, Bill S. Hansson, Rickard Ignell

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Female Aedes aegypti are vectors of dengue and yellow fever. Odor volatiles are the predominant cues that drive the host-seeking behavior of Ae. aegypti. Odorant molecules are detected and discriminated by olfactory receptor neurons (ORNs) housed in sensory hairs, sensilla, located on the antennae and maxillary palps. In a previous study, we used odor volatiles that are behaviorally and/or electrophysiologically active for Ae. aegypti and other mosquito species to show that antennal ORNs of female Ae. aegypti are divided into functionally different classes. In the present study, we have, for the first time, conducted gas chromatography-coupled single sensillum recordings (GC-SSR) from antennal trichoid and intermediate sensilla of female Ae. aegypti in order to screen for additional putative host attractants and repellents. We used headspace collections from biologically relevant sources, such as different human body parts (including feet, trunk regions and armpit), as well as a plant species used as a mosquito repellent, Nepeta faassenii. We found that a number of ORN types strongly responded to one or more of the biological extracts. GC-SSR recordings revealed several active components, which ware subsequently identified through GC-linked mass spectrometry (GC-MS). Electrophysiologically active volatiles from human skin included heptanal, octanal, nonanal and decanal.

Original languageEnglish (US)
Pages (from-to)3020-3027
Number of pages8
JournalJournal of Experimental Biology
Volume211
Issue number18
DOIs
StatePublished - Sep 1 2008
Externally publishedYes

Fingerprint

Olfactory Receptor Neurons
Sensilla
olfactory receptors
Aedes
sensilla
Aedes aegypti
Culicidae
mosquito
Gas Chromatography
odor
ligand
gas chromatography
neurons
odors
Ligands
Human Body
yellow fever
dengue fever
repellents
Nepeta

Keywords

  • Aedes aegypti
  • Biologically active volatiles
  • Electrophysiology
  • Olfactory receptor neurons

ASJC Scopus subject areas

  • Medicine(all)
  • Physiology
  • Ecology, Evolution, Behavior and Systematics
  • Aquatic Science
  • Molecular Biology
  • Animal Science and Zoology
  • Insect Science

Cite this

Natural odor ligands for olfactory receptor neurons of the female mosquito Aedes aegypti : Use of gas chromatography-linked single sensillum recordings. / Ghaninia Tabarestani, Majid; Larsson, Mattias; Hansson, Bill S.; Ignell, Rickard.

In: Journal of Experimental Biology, Vol. 211, No. 18, 01.09.2008, p. 3020-3027.

Research output: Contribution to journalArticle

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