Natural calcium isotonic composition of urine as a marker of bone mineral balance

Joseph Skulan, Thomas Bullen, Ariel Anbar, J. Edward Puzas, Linda Shackelford, Adrian LeBlanc, Scott M. Smith

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Background: We investigated whether changes in the natural isotopic composition of calcium in human urine track changes in net bone mineral balance, as predicted by a model of calcium isotopic behavior in vertebrates. If so, isotopic analysis of natural urine or blood calcium could be used to monitor short-term changes in bone mineral balance that cannot be detected with other techniques. Methods: Calcium isotopic compositions are expressed as δ44Ca, or the difference in parts per thousand between the 44Ca/40Ca of a sample and the 44Ca/ 40Ca of a standard reference material. δ44Ca was measured in urine samples from 10 persons who participated in a study of the effectiveness of countermeasures to bone loss in spaceflight, in which 17 weeks of bed rest was used to induce bone loss. Study participants were assigned to 1 of 3 treatment groups: controls received no treatment, one treatment group received alendronate, and another group performed resistive exercise. Measurements were made on urine samples collected before, at 2 or 3 points during, and after bed rest. Results: Urine δ44Ca values during bed rest were lower in controls than in individuals treated with alendronate (P <0.05, ANOVA) or exercise (P <0.05), and lower than the control group baseline (P <0.05, Mest). Results were consistent with the model and with biochemical and bone mineral density data. Conclusion: Results confirm the predicted relationship between bone mineral balance and calcium isotopes, suggesting that calcium isotopic analysis of urine might be refined into a clinical and research tool.

Original languageEnglish (US)
Pages (from-to)1155-1158
Number of pages4
JournalClinical Chemistry
Volume53
Issue number6
DOIs
StatePublished - Jun 2007

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Minerals
Bone
Urine
Calcium
Bone and Bones
Bed Rest
Chemical analysis
Alendronate
Calcium Isotopes
Space Flight
Control Groups
Analysis of variance (ANOVA)
Bone Density
Vertebrates
Analysis of Variance
Blood
Therapeutics
Research

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

Skulan, J., Bullen, T., Anbar, A., Puzas, J. E., Shackelford, L., LeBlanc, A., & Smith, S. M. (2007). Natural calcium isotonic composition of urine as a marker of bone mineral balance. Clinical Chemistry, 53(6), 1155-1158. https://doi.org/10.1373/clinchem.2006.080143

Natural calcium isotonic composition of urine as a marker of bone mineral balance. / Skulan, Joseph; Bullen, Thomas; Anbar, Ariel; Puzas, J. Edward; Shackelford, Linda; LeBlanc, Adrian; Smith, Scott M.

In: Clinical Chemistry, Vol. 53, No. 6, 06.2007, p. 1155-1158.

Research output: Contribution to journalArticle

Skulan, J, Bullen, T, Anbar, A, Puzas, JE, Shackelford, L, LeBlanc, A & Smith, SM 2007, 'Natural calcium isotonic composition of urine as a marker of bone mineral balance', Clinical Chemistry, vol. 53, no. 6, pp. 1155-1158. https://doi.org/10.1373/clinchem.2006.080143
Skulan, Joseph ; Bullen, Thomas ; Anbar, Ariel ; Puzas, J. Edward ; Shackelford, Linda ; LeBlanc, Adrian ; Smith, Scott M. / Natural calcium isotonic composition of urine as a marker of bone mineral balance. In: Clinical Chemistry. 2007 ; Vol. 53, No. 6. pp. 1155-1158.
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