TY - JOUR
T1 - Nanowire transistor arrays for mapping neural circuits in acute brain slices
AU - Qing, Quan
AU - Pal, Sumon K.
AU - Tian, Bozhi
AU - Duan, Xiaojie
AU - Timko, Brian P.
AU - Cohen-Karni, Tzahi
AU - Murthy, Venkatesh N.
AU - Lieber, Charles M.
PY - 2010/2/2
Y1 - 2010/2/2
N2 - Revealing the functional connectivity in natural neuronal networks is central to understanding circuits in the brain. Here,we show that silicon nanowire field-effect transistor (Si NWFET) arrays fabricated on transparent substrates can be reliably interfaced to acute brain slices. NWFETarrays were readily designed to record across a wide range of length scales, while the transparent device chips enabled imaging of individual cell bodies and identification of areas of healthy neurons at both upper and lower tissue surfaces. Simultaneous NWFET and patch clamp studies enabled unambiguous identification of action potential signals, with additional features detected at earlier times by the nanodevices. NWFET recording at different positions in the absence and presence of synaptic and ion-channel blockers enabled assignment of these features to presynaptic firing and postsynaptic depolarization from regions either close to somata or abundant in dendritic projections. In all cases, the NWFET signal amplitudes were from 0.3-3 mV. In contrast to conventional multielectrode array measurements, the small active surface of the NWFET devices, ∼0.06 μm2, provides highly localized multiplexed measurements of neuronal activities with demonstrated sub-millisecond temporal resolution and, significantly, better than 30 μm spatial resolution. In addition, multiplexed mapping with 2D NWFETarrays revealed spatially heterogeneous functional connectivity in the olfactory cortex with a resolution surpassing substantially previous electrical recording techniques. Our demonstration of simultaneous high temporal and spatial resolution recording, as well as mapping of functional connectivity, suggest that NWFETs can become a powerful platform for studying neural circuits in the brain.
AB - Revealing the functional connectivity in natural neuronal networks is central to understanding circuits in the brain. Here,we show that silicon nanowire field-effect transistor (Si NWFET) arrays fabricated on transparent substrates can be reliably interfaced to acute brain slices. NWFETarrays were readily designed to record across a wide range of length scales, while the transparent device chips enabled imaging of individual cell bodies and identification of areas of healthy neurons at both upper and lower tissue surfaces. Simultaneous NWFET and patch clamp studies enabled unambiguous identification of action potential signals, with additional features detected at earlier times by the nanodevices. NWFET recording at different positions in the absence and presence of synaptic and ion-channel blockers enabled assignment of these features to presynaptic firing and postsynaptic depolarization from regions either close to somata or abundant in dendritic projections. In all cases, the NWFET signal amplitudes were from 0.3-3 mV. In contrast to conventional multielectrode array measurements, the small active surface of the NWFET devices, ∼0.06 μm2, provides highly localized multiplexed measurements of neuronal activities with demonstrated sub-millisecond temporal resolution and, significantly, better than 30 μm spatial resolution. In addition, multiplexed mapping with 2D NWFETarrays revealed spatially heterogeneous functional connectivity in the olfactory cortex with a resolution surpassing substantially previous electrical recording techniques. Our demonstration of simultaneous high temporal and spatial resolution recording, as well as mapping of functional connectivity, suggest that NWFETs can become a powerful platform for studying neural circuits in the brain.
KW - Multiplexed recording
KW - Nanodevice
KW - Neuron
KW - Patch clamp
KW - Silicon
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U2 - 10.1073/pnas.0914737107
DO - 10.1073/pnas.0914737107
M3 - Article
C2 - 20133836
AN - SCOPUS:76649126436
SN - 0027-8424
VL - 107
SP - 1882
EP - 1887
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 5
ER -