TY - JOUR
T1 - Nanotexture optimization by oxygen plasma of mesoporous silica thin film for enrichment of low molecular weight peptides captured from human serum
AU - Hu, Ye
AU - Peng, Yang
AU - Brousseau, Louis
AU - Bouamrani, Ali
AU - Liu, Xuewu
AU - Ferrari, Mauro
N1 - Funding Information:
The authors thank the Microelectronic Research Center (MRC) at the University of Texas at Austin. We thank Dr. Domingo, the facility manager in the MRC, for providing the assistance in TEM operation. We also thank Dr. Paul Ho and Dr. Huangliang Shi for their help in FTIR measurement. These studies were supported by the following grants issued in the US: State of Texas's Emerging Technology Fund, National Institute of Health (NIH) (R21/R33CA122864, R01CA128797), National Aeronautics and Space Administration (NASA) (NNJ06HE06A), Department of Defense (DoD) Breast Cancer Research Program Innovator Award (W81XWH-09-1-0212). The authors would like to recognize the contributions and support from the Alliance for NanoHealth (ANH).
PY - 2010/11
Y1 - 2010/11
N2 - The optimization of mesoporous silica thin films by nanotexturing using oxygen plasma versus thermal oxidation was investigated. Calcination in oxygen plasma provides superior control over pore formation with regard to the pore surface and higher fidelity to the structure of the polymer template. The resulting porous film offers an ideal substrate for the selective partitioning of peptides from complex mixtures. The improved chemico-physical characteristics of porous thin films (pore size distribution, nanostructure, surface properties and pore connectivity) were systematically characterized with XRD, Ellipsometry, FTIR, TEM and N2 adsorption/desorption isotherm. The enrichment of low molecular weight proteins captured from human serum on mesoporous silica thin films fabricated by both methodologies was investigated by comparison of their MALDI-TOF MS profiles. This novel on-chip fractionation technology offers advantages in recovering the low molecular weight peptides from human serum, which has been recognized as an informative resource for early diagnosis of cancer and other diseases.
AB - The optimization of mesoporous silica thin films by nanotexturing using oxygen plasma versus thermal oxidation was investigated. Calcination in oxygen plasma provides superior control over pore formation with regard to the pore surface and higher fidelity to the structure of the polymer template. The resulting porous film offers an ideal substrate for the selective partitioning of peptides from complex mixtures. The improved chemico-physical characteristics of porous thin films (pore size distribution, nanostructure, surface properties and pore connectivity) were systematically characterized with XRD, Ellipsometry, FTIR, TEM and N2 adsorption/desorption isotherm. The enrichment of low molecular weight proteins captured from human serum on mesoporous silica thin films fabricated by both methodologies was investigated by comparison of their MALDI-TOF MS profiles. This novel on-chip fractionation technology offers advantages in recovering the low molecular weight peptides from human serum, which has been recognized as an informative resource for early diagnosis of cancer and other diseases.
KW - low molecular weight proteome
KW - mesoporous silica thin film
KW - oxygen plasma
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U2 - 10.1007/s11426-010-4121-x
DO - 10.1007/s11426-010-4121-x
M3 - Article
AN - SCOPUS:78649390598
SN - 1674-7291
VL - 53
SP - 2257
EP - 2264
JO - Science China Chemistry
JF - Science China Chemistry
IS - 11
ER -