Nanotexture optimization by oxygen plasma of mesoporous silica thin film for enrichment of low molecular weight peptides captured from human serum

Ye Hu, Yang Peng, Louis Brousseau, Ali Bouamrani, Xuewu Liu, Mauro Ferrari

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

The optimization of mesoporous silica thin films by nanotexturing using oxygen plasma versus thermal oxidation was investigated. Calcination in oxygen plasma provides superior control over pore formation with regard to the pore surface and higher fidelity to the structure of the polymer template. The resulting porous film offers an ideal substrate for the selective partitioning of peptides from complex mixtures. The improved chemico-physical characteristics of porous thin films (pore size distribution, nanostructure, surface properties and pore connectivity) were systematically characterized with XRD, Ellipsometry, FTIR, TEM and N2 adsorption/desorption isotherm. The enrichment of low molecular weight proteins captured from human serum on mesoporous silica thin films fabricated by both methodologies was investigated by comparison of their MALDI-TOF MS profiles. This novel on-chip fractionation technology offers advantages in recovering the low molecular weight peptides from human serum, which has been recognized as an informative resource for early diagnosis of cancer and other diseases.

Original languageEnglish (US)
Pages (from-to)2257-2264
Number of pages8
JournalScience China Chemistry
Volume53
Issue number11
DOIs
StatePublished - Nov 2010

Keywords

  • low molecular weight proteome
  • mesoporous silica thin film
  • oxygen plasma

ASJC Scopus subject areas

  • Chemistry(all)

Fingerprint Dive into the research topics of 'Nanotexture optimization by oxygen plasma of mesoporous silica thin film for enrichment of low molecular weight peptides captured from human serum'. Together they form a unique fingerprint.

  • Cite this