Nanopore protein biosensor using diffusive flow

A nanopore protein biosensor using a copolymer and diffusive flow of the test sample was developed. The copolymer coated within the nanopore can capture the antibody, which then captures the antigen. Diffusive flow of a test sample can be obtained using a concentration gradient between the nanopore and the micropore sides. No applied voltage was used to create the electrokinetic flow of the test liquid. The present nanopore protein biosensor showed high sensitivity and detected antigen quantities as low as 0.15 nM (4 pg/mL).