Nanopore film based enrichment and quantification of low abundance hepcidin from human bodily fluids

Jia Fan, Shiwen Niu, Ailian Dong, Jian Shi, Hung Jen Wu, Daniel H. Fine, Yaping Tian, Chunxi Zhou, Xuewu Liu, Tong Sun, Gregory J. Anderson, Mauro Ferrari, Guangjun Nie, Ye Hu, Yuliang Zhao

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Endogenous peptides that represent biological and pathological information of disease have attracted interest for diagnosis. However, the extraction of those low abundance peptides is still a challenge because of the complexity of human bodily fluids (HBF). Hepcidin, a peptide hormone, has been recognized as a biomarker for iron-related diseases. There is no rapid and reliable way to enrich them from HBF. Here we describe a peptide extraction approach based on nanoporous silica thin films to successfully detect hepcidin from HBF. Cooperative functions of nanopore to biomolecule, including capillary adsorption, size-exclusion and electrostatic interaction, were systematically investigated to immobilize the target peptide. To promote this new approach to clinical practices, we further applied it to successfully assay the hepcidin levels in HBF provided by healthy volunteers and patients suffering from inflammation. Our finding provides a high-throughput, rapid, label-free and cost-effective detection method for capturing and quantifying low abundance peptides from HBF. From the Clinical Editor: Diagnosing diseases with low concentration peptide biomarkers remains challenging. This team of authors describes a peptide extraction approach based on nanoporous silica thin films to successfully detect low concentrations of hepcidin from human body fluids collected from 119 healthy volunteers and 19 inflammation patients.

Original languageEnglish (US)
Pages (from-to)879-888
Number of pages10
JournalNanomedicine: Nanotechnology, Biology, and Medicine
Volume10
Issue number5
DOIs
StatePublished - Jan 1 2014
Externally publishedYes

Fingerprint

Nanopores
Hepcidins
Peptides
Fluids
Biomarkers
Silicon Dioxide
Healthy Volunteers
Silica
Inflammation
Thin films
Peptide Hormones
Body fluids
Body Fluids
Biomolecules
Coulomb interactions
Static Electricity
Human Body
Adsorption
Labels
Assays

Keywords

  • Biomarker discovery
  • Hepcidin
  • MALDI-TOF MS
  • Nanoporous silica film
  • Peptide

ASJC Scopus subject areas

  • Bioengineering
  • Medicine (miscellaneous)
  • Molecular Medicine
  • Biomedical Engineering
  • Materials Science(all)
  • Pharmaceutical Science

Cite this

Nanopore film based enrichment and quantification of low abundance hepcidin from human bodily fluids. / Fan, Jia; Niu, Shiwen; Dong, Ailian; Shi, Jian; Wu, Hung Jen; Fine, Daniel H.; Tian, Yaping; Zhou, Chunxi; Liu, Xuewu; Sun, Tong; Anderson, Gregory J.; Ferrari, Mauro; Nie, Guangjun; Hu, Ye; Zhao, Yuliang.

In: Nanomedicine: Nanotechnology, Biology, and Medicine, Vol. 10, No. 5, 01.01.2014, p. 879-888.

Research output: Contribution to journalArticle

Fan, J, Niu, S, Dong, A, Shi, J, Wu, HJ, Fine, DH, Tian, Y, Zhou, C, Liu, X, Sun, T, Anderson, GJ, Ferrari, M, Nie, G, Hu, Y & Zhao, Y 2014, 'Nanopore film based enrichment and quantification of low abundance hepcidin from human bodily fluids', Nanomedicine: Nanotechnology, Biology, and Medicine, vol. 10, no. 5, pp. 879-888. https://doi.org/10.1016/j.nano.2014.02.005
Fan, Jia ; Niu, Shiwen ; Dong, Ailian ; Shi, Jian ; Wu, Hung Jen ; Fine, Daniel H. ; Tian, Yaping ; Zhou, Chunxi ; Liu, Xuewu ; Sun, Tong ; Anderson, Gregory J. ; Ferrari, Mauro ; Nie, Guangjun ; Hu, Ye ; Zhao, Yuliang. / Nanopore film based enrichment and quantification of low abundance hepcidin from human bodily fluids. In: Nanomedicine: Nanotechnology, Biology, and Medicine. 2014 ; Vol. 10, No. 5. pp. 879-888.
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