Nanopore chip with self-aligned transverse tunneling junction for DNA detection

Yuan Wang, Joshua Sadar, Ching Wei Tsao, Sanjana Mukherjee, Quan Qing

Research output: Contribution to journalArticlepeer-review

Abstract

To achieve better signal quality and resolution in nanopore sequencing, there has been strong interest in quantum tunneling based detection which requires integration of tunneling junctions in nanopores. However, there has been very limited success due to precision and reproducibility issues. Here we report a new strategy based on feedback-controlled electrochemical processes in a confined nanoscale space to construct nanopore devices with self-aligned transverse tunneling junctions, all embedded on a nanofluidic chip. We demonstrate high-yield (>93%) correlated detection of translocating DNAs from both the ionic channel and the tunneling junction with enriched event rate. We also observed events attributed to non-translocating DNA making contact with the transverse electrodes. Existing challenges for precise sequencing are discussed, including fast translocation speed, and interference from transient electrostatic signals from fast-moving DNAs. Our work can serve as a first step to provide an accessible, and reproducible platform enabling further optimizations for tunneling-based DNA detection, and potentially sequencing.

Original languageEnglish (US)
Article number113552
JournalBiosensors and Bioelectronics
Volume193
DOIs
StatePublished - Dec 1 2021
Externally publishedYes

Keywords

  • DNA Sequencing
  • Electrochemical deposition
  • Nanofluidic
  • Nanopore
  • Tunneling junction

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

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