Nanoparticle-based sensing of glycan-lectin interactions

Zong Dai; Abdel Nasser Kawde; Yun Xiang; Jeffrey LaBelle; Jared Gerlach; Veer P. Bhavanandan; Lokesh Joshi; Joseph Wang

doi:10.1021/ja063565p

Nanoparticle-based sensing of glycan-lectin interactions

Zong Dai, Abdel Nasser Kawde, Yun Xiang, Jeffrey LaBelle, Jared Gerlach, Veer P. Bhavanandan, Lokesh Joshi, Joseph Wang

Bioengineering, Harrington Department of

Research output: Contribution to journal › Article › peer-review

116 Scopus citations

Abstract

Here we present the first report on nanoparticle-based biosensing of glycan markers of diseases. The protocol relies on the competition between a nanocrystal (CdS)-tagged sugar and the target sugar for the binding sites of surface-confined lectin and monitoring the extent of competition through highly sensitive electrochemical detection of the captured nanocrystal. This development is expected to allow decentralized detection of carbohydrate moieties and lectin-carbohydrate interactions to be performed more rapidly, sensitively, inexpensively, and reliably.

Original language	English (US)
Pages (from-to)	10018-10019
Number of pages	2
Journal	Journal of the American Chemical Society
Volume	128
Issue number	31
DOIs	https://doi.org/10.1021/ja063565p
State	Published - Aug 9 2006

ASJC Scopus subject areas

Catalysis
Chemistry(all)
Biochemistry
Colloid and Surface Chemistry

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abstract = "Here we present the first report on nanoparticle-based biosensing of glycan markers of diseases. The protocol relies on the competition between a nanocrystal (CdS)-tagged sugar and the target sugar for the binding sites of surface-confined lectin and monitoring the extent of competition through highly sensitive electrochemical detection of the captured nanocrystal. This development is expected to allow decentralized detection of carbohydrate moieties and lectin-carbohydrate interactions to be performed more rapidly, sensitively, inexpensively, and reliably.",

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AU - Dai, Zong

AU - Kawde, Abdel Nasser

AU - Xiang, Yun

AU - LaBelle, Jeffrey

AU - Gerlach, Jared

AU - Bhavanandan, Veer P.

AU - Joshi, Lokesh

AU - Wang, Joseph

PY - 2006/8/9

Y1 - 2006/8/9

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AB - Here we present the first report on nanoparticle-based biosensing of glycan markers of diseases. The protocol relies on the competition between a nanocrystal (CdS)-tagged sugar and the target sugar for the binding sites of surface-confined lectin and monitoring the extent of competition through highly sensitive electrochemical detection of the captured nanocrystal. This development is expected to allow decentralized detection of carbohydrate moieties and lectin-carbohydrate interactions to be performed more rapidly, sensitively, inexpensively, and reliably.

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Nanoparticle-based sensing of glycan-lectin interactions

Abstract

ASJC Scopus subject areas

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