Nanoflow electrospinning serial femtosecond crystallography

Raymond G. Sierra; Hartawan Laksmono; Jan Kern; Rosalie Tran; Johan Hattne; Roberto Alonso-Mori; Benedikt Lassalle-Kaiser; Carina Glöckner; Julia Hellmich; Donald W. Schafer; Nathaniel Echols; Richard J. Gildea; Ralf W. Grosse-Kunstleve; Jonas Sellberg; Trevor A. McQueen; Alan R. Fry; Marc M. Messerschmidt; Alan Miahnahri; M. Marvin Seibert; Christina Y. Hampton; Dmitri Starodub; N. Duane Loh; Dimosthenis Sokaras; Tsu Chien Weng; Petrus H. Zwart; Pieter Glatzel; Despina Milathianaki; William E. White; Paul D. Adams; Garth J. Williams; Sébastien Boutet; Athina Zouni; Johannes Messinger; Nicholas K. Sauter; Uwe Bergmann; Junko Yano; Vittal K. Yachandra; Michael J. Bogan

doi:10.1107/S0907444912038152

Nanoflow electrospinning serial femtosecond crystallography

Raymond G. Sierra, Hartawan Laksmono, Jan Kern, Rosalie Tran, Johan Hattne, Roberto Alonso-Mori, Benedikt Lassalle-Kaiser, Carina Glöckner, Julia Hellmich, Donald W. Schafer, Nathaniel Echols, Richard J. Gildea, Ralf W. Grosse-Kunstleve, Jonas Sellberg, Trevor A. McQueen, Alan R. Fry, Marc M. Messerschmidt, Alan Miahnahri, M. Marvin Seibert, Christina Y. HamptonDmitri Starodub, N. Duane Loh, Dimosthenis Sokaras, Tsu Chien Weng, Petrus H. Zwart, Pieter Glatzel, Despina Milathianaki, William E. White, Paul D. Adams, Garth J. Williams, Sébastien Boutet, Athina Zouni, Johannes Messinger, Nicholas K. Sauter, Uwe Bergmann, Junko Yano, Vittal K. Yachandra, Michael J. Bogan

Research output: Contribution to journal › Article › peer-review

161 Scopus citations

Abstract

An electrospun liquid microjet has been developed that delivers protein microcrystal suspensions at flow rates of 0.14-3.1 l min^-1 to perform serial femtosecond crystallography (SFX) studies with X-ray lasers. Thermolysin microcrystals flowed at 0.17 l min^-1 and diffracted to beyond 4 Å resolution, producing 14 000 indexable diffraction patterns, or four per second, from 140 g of protein. Nanoflow electrospinning extends SFX to biological samples that necessitate minimal sample consumption.

Original language	English (US)
Pages (from-to)	1584-1587
Number of pages	4
Journal	Acta Crystallographica Section D: Biological Crystallography
Volume	68
Issue number	11
DOIs	https://doi.org/10.1107/S0907444912038152
State	Published - Nov 2012
Externally published	Yes

Keywords

nanoflow electrospinning
serial femtosecond crystallography

ASJC Scopus subject areas

Structural Biology

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10.1107/S0907444912038152

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Sierra, R. G., Laksmono, H., Kern, J., Tran, R., Hattne, J., Alonso-Mori, R., Lassalle-Kaiser, B., Glöckner, C., Hellmich, J., Schafer, D. W., Echols, N., Gildea, R. J., Grosse-Kunstleve, R. W., Sellberg, J., McQueen, T. A., Fry, A. R., Messerschmidt, M. M., Miahnahri, A., Seibert, M. M., ... Bogan, M. J. (2012). Nanoflow electrospinning serial femtosecond crystallography. Acta Crystallographica Section D: Biological Crystallography, 68(11), 1584-1587. https://doi.org/10.1107/S0907444912038152

Sierra, RG, Laksmono, H, Kern, J, Tran, R, Hattne, J, Alonso-Mori, R, Lassalle-Kaiser, B, Glöckner, C, Hellmich, J, Schafer, DW, Echols, N, Gildea, RJ, Grosse-Kunstleve, RW, Sellberg, J, McQueen, TA, Fry, AR, Messerschmidt, MM, Miahnahri, A, Seibert, MM, Hampton, CY, Starodub, D, Loh, ND, Sokaras, D, Weng, TC, Zwart, PH, Glatzel, P, Milathianaki, D, White, WE, Adams, PD, Williams, GJ, Boutet, S, Zouni, A, Messinger, J, Sauter, NK, Bergmann, U, Yano, J, Yachandra, VK & Bogan, MJ 2012, 'Nanoflow electrospinning serial femtosecond crystallography', Acta Crystallographica Section D: Biological Crystallography, vol. 68, no. 11, pp. 1584-1587. https://doi.org/10.1107/S0907444912038152

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abstract = "An electrospun liquid microjet has been developed that delivers protein microcrystal suspensions at flow rates of 0.14-3.1 l min-1 to perform serial femtosecond crystallography (SFX) studies with X-ray lasers. Thermolysin microcrystals flowed at 0.17 l min-1 and diffracted to beyond 4 {\AA} resolution, producing 14 000 indexable diffraction patterns, or four per second, from 140 g of protein. Nanoflow electrospinning extends SFX to biological samples that necessitate minimal sample consumption.",

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language = "English (US)",

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AU - Sierra, Raymond G.

AU - Laksmono, Hartawan

AU - Kern, Jan

AU - Tran, Rosalie

AU - Hattne, Johan

AU - Alonso-Mori, Roberto

AU - Lassalle-Kaiser, Benedikt

AU - Glöckner, Carina

AU - Hellmich, Julia

AU - Schafer, Donald W.

AU - Echols, Nathaniel

AU - Gildea, Richard J.

AU - Grosse-Kunstleve, Ralf W.

AU - Sellberg, Jonas

AU - McQueen, Trevor A.

AU - Fry, Alan R.

AU - Messerschmidt, Marc M.

AU - Miahnahri, Alan

AU - Seibert, M. Marvin

AU - Hampton, Christina Y.

AU - Starodub, Dmitri

AU - Loh, N. Duane

AU - Sokaras, Dimosthenis

AU - Weng, Tsu Chien

AU - Zwart, Petrus H.

AU - Glatzel, Pieter

AU - Milathianaki, Despina

AU - White, William E.

AU - Adams, Paul D.

AU - Williams, Garth J.

AU - Boutet, Sébastien

AU - Zouni, Athina

AU - Messinger, Johannes

AU - Sauter, Nicholas K.

AU - Bergmann, Uwe

AU - Yano, Junko

AU - Yachandra, Vittal K.

AU - Bogan, Michael J.

PY - 2012/11

Y1 - 2012/11

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Nanoflow electrospinning serial femtosecond crystallography

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