Nanoengineered glycan sensors enabling native glycoprofiling for medicinal applications: Towards profiling glycoproteins without labeling or liberation steps

Nigel F. Reuel, Bin Mu, Jingqing Zhang, Allison Hinckley, Michael S. Strano

Research output: Contribution to journalArticlepeer-review

41 Scopus citations

Abstract

Nanoengineered glycan sensors may help realize the long-held goal of accurate and rapid glycoprotein profiling without labeling or glycan liberation steps. Current methods of profiling oligosaccharides displayed on protein surfaces, such as liquid chromatography, mass spectrometry, capillary electrophoresis, and microarray methods, are limited by sample pretreatment and quantitative accuracy. Microarrayed platforms can be improved with methods that better estimate kinetic parameters rather than simply reporting relative binding information. These quantitative glycan sensors are enabled by an emerging class of nanoengineered materials that differ in their mode of signal transduction from traditional methods. Platforms that respond to mass changes include a quartz crystal microbalance and cantilever sensors. Electronic response can be detected from electrochemical, field effect transistor, and pore impedance sensors. Optical methods include fluorescent frontal affinity chromatography, surface plasmon resonance methods, and fluorescent carbon nanotubes. After a very brief primer on glycobiology and its connection to medicine, these emerging systems are critically reviewed for their potential use as core sensors in future glycoprofiling tools.

Original languageEnglish (US)
Pages (from-to)5744-5779
Number of pages36
JournalChemical Society Reviews
Volume41
Issue number17
DOIs
StatePublished - Aug 6 2012
Externally publishedYes

ASJC Scopus subject areas

  • Chemistry(all)

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