Nanoarrays for single-cell RNA Expression

Stuart Lindsay (Inventor), Hao Yan (Inventor), Peiming Zhang (Inventor)

Research output: Patent

Abstract

A variety of medical ailments can be characterized by the presence of specific nucleic acids, proteins, and lipids that are generated during the course of the ailment. Multiplexed and sensitive detection of these molecules is of great importance in the medical diagnostics field. Current technologies designed to detect biological events coded by specific macromolecules typically consist of chip-based or particle-based platforms incorporating a large number of designed probes for proteins and nucleic acids. Chemically synthesized DNA oligonucleotides can self-assemble into ordered nucleic acid tiling arrays with controlled distances and fixed spatial orientations. These arrays can be used in a variety of biomedical applications. Researchers at Arizona State University have developed a method for making nanoscale arrays from self-assembly of DNA in such a way as to expose single stranded probes. The array orientation and probe can be imaged by atomic force microscopy. More importantly, several improvements were implemented allowing the arrays to be used directly inside the contents of single cells or with very small volumes of sample. These improvements enhanced the inventions ability to detect RNA expression of a single cell, with accuracy. Potential Applications This technology can be used for a variety of applications involving detection of different biological molecules: Medical Diagnostics Identify biological molecules associated with medical problems, organ and disease specific arrays Proteomics Expression profiling Genetic assays Gene expression, SNP profiling, nucleic acid sequencingBenefits and Advantages Versatility Allows simultaneous identification of proteins, nucleic acids, and lipids from a single sample solution Ease of Use Water solubility, fast binding kinetics of molecular probes to targets, rechargeability for repeated use, and small sample volumes make this technology easy to use Dowload Original PDFFor more information about the inventor(s) and their research, please see Dr. Yan's laboratory webpageDr. Chaput's laboratory webpageDr. Zhang's directory webpage Dr. Lindsay's laboratory webpage
Original languageEnglish (US)
StatePublished - Jan 27 2006

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Nucleic Acids
RNA
Molecules
Lipids
Molecular Probes
Proteins
DNA
Patents and inventions
Medical problems
Macromolecules
Gene expression
Oligonucleotides
Self assembly
Assays
Atomic force microscopy
Solubility
Kinetics
Water

Cite this

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title = "Nanoarrays for single-cell RNA Expression",
abstract = "A variety of medical ailments can be characterized by the presence of specific nucleic acids, proteins, and lipids that are generated during the course of the ailment. Multiplexed and sensitive detection of these molecules is of great importance in the medical diagnostics field. Current technologies designed to detect biological events coded by specific macromolecules typically consist of chip-based or particle-based platforms incorporating a large number of designed probes for proteins and nucleic acids. Chemically synthesized DNA oligonucleotides can self-assemble into ordered nucleic acid tiling arrays with controlled distances and fixed spatial orientations. These arrays can be used in a variety of biomedical applications. Researchers at Arizona State University have developed a method for making nanoscale arrays from self-assembly of DNA in such a way as to expose single stranded probes. The array orientation and probe can be imaged by atomic force microscopy. More importantly, several improvements were implemented allowing the arrays to be used directly inside the contents of single cells or with very small volumes of sample. These improvements enhanced the inventions ability to detect RNA expression of a single cell, with accuracy. Potential Applications This technology can be used for a variety of applications involving detection of different biological molecules: Medical Diagnostics Identify biological molecules associated with medical problems, organ and disease specific arrays Proteomics Expression profiling Genetic assays Gene expression, SNP profiling, nucleic acid sequencingBenefits and Advantages Versatility Allows simultaneous identification of proteins, nucleic acids, and lipids from a single sample solution Ease of Use Water solubility, fast binding kinetics of molecular probes to targets, rechargeability for repeated use, and small sample volumes make this technology easy to use Dowload Original PDFFor more information about the inventor(s) and their research, please see Dr. Yan's laboratory webpageDr. Chaput's laboratory webpageDr. Zhang's directory webpage Dr. Lindsay's laboratory webpage",
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T1 - Nanoarrays for single-cell RNA Expression

AU - Lindsay, Stuart

AU - Yan, Hao

AU - Zhang, Peiming

PY - 2006/1/27

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N2 - A variety of medical ailments can be characterized by the presence of specific nucleic acids, proteins, and lipids that are generated during the course of the ailment. Multiplexed and sensitive detection of these molecules is of great importance in the medical diagnostics field. Current technologies designed to detect biological events coded by specific macromolecules typically consist of chip-based or particle-based platforms incorporating a large number of designed probes for proteins and nucleic acids. Chemically synthesized DNA oligonucleotides can self-assemble into ordered nucleic acid tiling arrays with controlled distances and fixed spatial orientations. These arrays can be used in a variety of biomedical applications. Researchers at Arizona State University have developed a method for making nanoscale arrays from self-assembly of DNA in such a way as to expose single stranded probes. The array orientation and probe can be imaged by atomic force microscopy. More importantly, several improvements were implemented allowing the arrays to be used directly inside the contents of single cells or with very small volumes of sample. These improvements enhanced the inventions ability to detect RNA expression of a single cell, with accuracy. Potential Applications This technology can be used for a variety of applications involving detection of different biological molecules: Medical Diagnostics Identify biological molecules associated with medical problems, organ and disease specific arrays Proteomics Expression profiling Genetic assays Gene expression, SNP profiling, nucleic acid sequencingBenefits and Advantages Versatility Allows simultaneous identification of proteins, nucleic acids, and lipids from a single sample solution Ease of Use Water solubility, fast binding kinetics of molecular probes to targets, rechargeability for repeated use, and small sample volumes make this technology easy to use Dowload Original PDFFor more information about the inventor(s) and their research, please see Dr. Yan's laboratory webpageDr. Chaput's laboratory webpageDr. Zhang's directory webpage Dr. Lindsay's laboratory webpage

AB - A variety of medical ailments can be characterized by the presence of specific nucleic acids, proteins, and lipids that are generated during the course of the ailment. Multiplexed and sensitive detection of these molecules is of great importance in the medical diagnostics field. Current technologies designed to detect biological events coded by specific macromolecules typically consist of chip-based or particle-based platforms incorporating a large number of designed probes for proteins and nucleic acids. Chemically synthesized DNA oligonucleotides can self-assemble into ordered nucleic acid tiling arrays with controlled distances and fixed spatial orientations. These arrays can be used in a variety of biomedical applications. Researchers at Arizona State University have developed a method for making nanoscale arrays from self-assembly of DNA in such a way as to expose single stranded probes. The array orientation and probe can be imaged by atomic force microscopy. More importantly, several improvements were implemented allowing the arrays to be used directly inside the contents of single cells or with very small volumes of sample. These improvements enhanced the inventions ability to detect RNA expression of a single cell, with accuracy. Potential Applications This technology can be used for a variety of applications involving detection of different biological molecules: Medical Diagnostics Identify biological molecules associated with medical problems, organ and disease specific arrays Proteomics Expression profiling Genetic assays Gene expression, SNP profiling, nucleic acid sequencingBenefits and Advantages Versatility Allows simultaneous identification of proteins, nucleic acids, and lipids from a single sample solution Ease of Use Water solubility, fast binding kinetics of molecular probes to targets, rechargeability for repeated use, and small sample volumes make this technology easy to use Dowload Original PDFFor more information about the inventor(s) and their research, please see Dr. Yan's laboratory webpageDr. Chaput's laboratory webpageDr. Zhang's directory webpage Dr. Lindsay's laboratory webpage

M3 - Patent

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