TY - JOUR
T1 - N-acyl-homoserine-lactones signaling as a critical control point for phosphorus entrapment by multi-species microbial aggregates
AU - Xu, Ying
AU - Curtis, Thomas
AU - Dolfing, Jan
AU - Wu, Yonghong
AU - Rittmann, Bruce E.
N1 - Funding Information:
This work was supported by the National Natural Science Foundation of China ( 41825021 , 41961144010 and 31772396 ), the Natural Science Foundation of Jiangsu Province , China ( BZ2019015 and BE2020731 ), and the Original Innovation Project of Chinese Academy of Sciences ( ZDBS-LY-DQC024 ).
Publisher Copyright:
© 2021 Elsevier Ltd
PY - 2021/10/1
Y1 - 2021/10/1
N2 - Quorum sensing (QS) has been extensively studied in pure stains of microorganisms, but the ecological roles of QS in multi-species microbial aggregates are poorly understood due to the aggregates’ heterogeneity and complexity, in particular the phosphorus (P) entrapment, a key aspect of element cycling. Using periphytic biofilm as a microbial-aggregate model, we addressed how QS signaling via N-acyl-homoserine-lactones (AHLs) regulated P entrapment. The most-abundant AHLs detected were C8-HSL, 3OC8-HSL, and C12-HSL, are the primary regulator of P entrapment in the periphytic biofilm. QS signaling-AHL is a beneficial molecule for bacterial growth in periphytic biofilm and the addition of these three AHLs optimized polyphosphate accumulating organisms (PAOs) community. Growth promotion was accompanied by up-regulation of pyrimidine, purine and energy metabolism. Both intra- and extra-cellular P entrapment were enhanced in the addition of AHLs. AHLs increased extracellular polymeric substances (EPS) production to drive extracellular P entrapment, via up-regulating amino acids biosynthesis and amino sugar/nucleotide sugar metabolism. Also, AHLs improved intracellular P entrapment potential by regulating genes involved in inorganic-P accumulation (ppk, ppx) and P uptake and transport (pit, pstSCAB). This proof-of-concept evidence about how QS signaling regulates P entrapment by microbial aggregates paves the way for managing QS to enhance P removal by microbial aggregates in aquatic environments.
AB - Quorum sensing (QS) has been extensively studied in pure stains of microorganisms, but the ecological roles of QS in multi-species microbial aggregates are poorly understood due to the aggregates’ heterogeneity and complexity, in particular the phosphorus (P) entrapment, a key aspect of element cycling. Using periphytic biofilm as a microbial-aggregate model, we addressed how QS signaling via N-acyl-homoserine-lactones (AHLs) regulated P entrapment. The most-abundant AHLs detected were C8-HSL, 3OC8-HSL, and C12-HSL, are the primary regulator of P entrapment in the periphytic biofilm. QS signaling-AHL is a beneficial molecule for bacterial growth in periphytic biofilm and the addition of these three AHLs optimized polyphosphate accumulating organisms (PAOs) community. Growth promotion was accompanied by up-regulation of pyrimidine, purine and energy metabolism. Both intra- and extra-cellular P entrapment were enhanced in the addition of AHLs. AHLs increased extracellular polymeric substances (EPS) production to drive extracellular P entrapment, via up-regulating amino acids biosynthesis and amino sugar/nucleotide sugar metabolism. Also, AHLs improved intracellular P entrapment potential by regulating genes involved in inorganic-P accumulation (ppk, ppx) and P uptake and transport (pit, pstSCAB). This proof-of-concept evidence about how QS signaling regulates P entrapment by microbial aggregates paves the way for managing QS to enhance P removal by microbial aggregates in aquatic environments.
KW - N-acyl-homoserine-lactone
KW - Periphytic biofilm
KW - Phosphorus entrapment
KW - Phosphorus entrapment gene
KW - Quorum sensing
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U2 - 10.1016/j.watres.2021.117627
DO - 10.1016/j.watres.2021.117627
M3 - Article
C2 - 34509868
AN - SCOPUS:85114680527
SN - 0043-1354
VL - 204
JO - Water Research
JF - Water Research
M1 - 117627
ER -