TY - JOUR
T1 - Myxoma virus
T2 - Propagation, purification, quantification, and storage
AU - Smallwood, Sherin E.
AU - Rahman, Masmudur M.
AU - Smith, Dorothy W.
AU - McFadden, Grant
PY - 2010
Y1 - 2010
N2 - Myxoma virus (MYXV) is a member of the Poxviridae family and prototype for the genus Leporipoxvirus. It is pathogenic only for European rabbits, in which it causes the lethal disease myxomatosis, and two North American species, in which it causes a less severe disease. MYXV replicates exclusively in the cytoplasm of the host cell. Although not infectious in humans, its genome encodes proteins that can interfere with or modulate host defense mechanisms; it is able to productively infect a number of human cancer cell lines, but not normal human cells, and has also been shown to increase survival time in mouse models of human glioma. These characteristics suggest that MYXV could be a viable therapeutic agent, e.g., in anti-inflammatory or anti-immune therapy, or as an oncolytic agent. MYXV is also an excellent model for poxvirus biology, pathogenesis, and host tropism studies. It is easily propagated in a number of cell lines, including adherent cells and suspension cultures, and minimal purification is required to provide a stock for in vivo and in vitro studies.
AB - Myxoma virus (MYXV) is a member of the Poxviridae family and prototype for the genus Leporipoxvirus. It is pathogenic only for European rabbits, in which it causes the lethal disease myxomatosis, and two North American species, in which it causes a less severe disease. MYXV replicates exclusively in the cytoplasm of the host cell. Although not infectious in humans, its genome encodes proteins that can interfere with or modulate host defense mechanisms; it is able to productively infect a number of human cancer cell lines, but not normal human cells, and has also been shown to increase survival time in mouse models of human glioma. These characteristics suggest that MYXV could be a viable therapeutic agent, e.g., in anti-inflammatory or anti-immune therapy, or as an oncolytic agent. MYXV is also an excellent model for poxvirus biology, pathogenesis, and host tropism studies. It is easily propagated in a number of cell lines, including adherent cells and suspension cultures, and minimal purification is required to provide a stock for in vivo and in vitro studies.
KW - Myxoma virus
KW - Poxvirus
KW - Sucrose gradient purification
KW - Titration
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U2 - 10.1002/9780471729259.mc14a01s17
DO - 10.1002/9780471729259.mc14a01s17
M3 - Review article
C2 - 20440681
AN - SCOPUS:77952210238
SN - 1934-8525
SP - 14A.1.1-14A.1.20
JO - Current Protocols in Microbiology
JF - Current Protocols in Microbiology
IS - SUPPL. 17
ER -