Myxoma virus M130R is a novel virulence factor required for lethal myxomatosis in rabbits

John W. Barrett; Steven J. Werden; Fuan Wang; William M. McKillop; June Jimenez; Danielle Villeneuve; Grant McFadden; Gregory A. Dekaban

doi:10.1016/j.virusres.2009.05.009

Myxoma virus M130R is a novel virulence factor required for lethal myxomatosis in rabbits

John W. Barrett, Steven J. Werden, Fuan Wang, William M. McKillop, June Jimenez, Danielle Villeneuve, Grant McFadden, Gregory A. Dekaban

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

Myxoma virus (MV) is a highly lethal, rabbit-specific poxvirus that induces a disease called myxomatosis in European rabbits. In an effort to understand the function of predicted immunomodulatory genes we have deleted various viral genes from MV and tested the ability of these knockout viruses to induce lethal myxomatosis. MV encodes a unique 15 kD cytoplasmic protein (M130R) that is expressed late (12 h post infection) during infection. M130R is a non-essential gene for MV replication in rabbit, monkey or human cell lines. Construction of a targeted gene knockout virus (vMyx130KO) and infection of susceptible rabbits demonstrate that the M130R knockout virus is attenuated and that loss of M130R expression allows the rabbit host immune system to effectively respond to and control the lethal effects of MV. M130R expression is a bona fide poxviral virulence factor necessary for full and lethal development of myxomatosis.

Original language	English (US)
Pages (from-to)	258-265
Number of pages	8
Journal	Virus research
Volume	144
Issue number	1-2
DOIs	https://doi.org/10.1016/j.virusres.2009.05.009
State	Published - Sep 2009
Externally published	Yes

Keywords

Host range
Host-interaction
Pathogenesis
Poxvirus

ASJC Scopus subject areas

Virology
Infectious Diseases
Cancer Research

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10.1016/j.virusres.2009.05.009

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@article{43302696e9284d729e42437a41992fca,

title = "Myxoma virus M130R is a novel virulence factor required for lethal myxomatosis in rabbits",

abstract = "Myxoma virus (MV) is a highly lethal, rabbit-specific poxvirus that induces a disease called myxomatosis in European rabbits. In an effort to understand the function of predicted immunomodulatory genes we have deleted various viral genes from MV and tested the ability of these knockout viruses to induce lethal myxomatosis. MV encodes a unique 15 kD cytoplasmic protein (M130R) that is expressed late (12 h post infection) during infection. M130R is a non-essential gene for MV replication in rabbit, monkey or human cell lines. Construction of a targeted gene knockout virus (vMyx130KO) and infection of susceptible rabbits demonstrate that the M130R knockout virus is attenuated and that loss of M130R expression allows the rabbit host immune system to effectively respond to and control the lethal effects of MV. M130R expression is a bona fide poxviral virulence factor necessary for full and lethal development of myxomatosis.",

keywords = "Host range, Host-interaction, Pathogenesis, Poxvirus",

author = "Barrett, {John W.} and Werden, {Steven J.} and Fuan Wang and McKillop, {William M.} and June Jimenez and Danielle Villeneuve and Grant McFadden and Dekaban, {Gregory A.}",

note = "Funding Information: This study was supported by the Canadian Institutes of Health Research. ",

year = "2009",

month = sep,

doi = "10.1016/j.virusres.2009.05.009",

language = "English (US)",

volume = "144",

pages = "258--265",

journal = "Virus research",

issn = "0168-1702",

publisher = "Elsevier",

number = "1-2",

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TY - JOUR

T1 - Myxoma virus M130R is a novel virulence factor required for lethal myxomatosis in rabbits

AU - Barrett, John W.

AU - Werden, Steven J.

AU - Wang, Fuan

AU - McKillop, William M.

AU - Jimenez, June

AU - Villeneuve, Danielle

AU - McFadden, Grant

AU - Dekaban, Gregory A.

N1 - Funding Information: This study was supported by the Canadian Institutes of Health Research.

PY - 2009/9

Y1 - 2009/9

N2 - Myxoma virus (MV) is a highly lethal, rabbit-specific poxvirus that induces a disease called myxomatosis in European rabbits. In an effort to understand the function of predicted immunomodulatory genes we have deleted various viral genes from MV and tested the ability of these knockout viruses to induce lethal myxomatosis. MV encodes a unique 15 kD cytoplasmic protein (M130R) that is expressed late (12 h post infection) during infection. M130R is a non-essential gene for MV replication in rabbit, monkey or human cell lines. Construction of a targeted gene knockout virus (vMyx130KO) and infection of susceptible rabbits demonstrate that the M130R knockout virus is attenuated and that loss of M130R expression allows the rabbit host immune system to effectively respond to and control the lethal effects of MV. M130R expression is a bona fide poxviral virulence factor necessary for full and lethal development of myxomatosis.

AB - Myxoma virus (MV) is a highly lethal, rabbit-specific poxvirus that induces a disease called myxomatosis in European rabbits. In an effort to understand the function of predicted immunomodulatory genes we have deleted various viral genes from MV and tested the ability of these knockout viruses to induce lethal myxomatosis. MV encodes a unique 15 kD cytoplasmic protein (M130R) that is expressed late (12 h post infection) during infection. M130R is a non-essential gene for MV replication in rabbit, monkey or human cell lines. Construction of a targeted gene knockout virus (vMyx130KO) and infection of susceptible rabbits demonstrate that the M130R knockout virus is attenuated and that loss of M130R expression allows the rabbit host immune system to effectively respond to and control the lethal effects of MV. M130R expression is a bona fide poxviral virulence factor necessary for full and lethal development of myxomatosis.

KW - Host range

KW - Host-interaction

KW - Pathogenesis

KW - Poxvirus

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U2 - 10.1016/j.virusres.2009.05.009

DO - 10.1016/j.virusres.2009.05.009

M3 - Article

C2 - 19477207

AN - SCOPUS:69249205407

SN - 0168-1702

VL - 144

SP - 258

EP - 265

JO - Virus research

JF - Virus research

IS - 1-2

ER -

Myxoma virus M130R is a novel virulence factor required for lethal myxomatosis in rabbits

Abstract

Keywords

ASJC Scopus subject areas

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