Mycobacterium tuberculosis response regulators, DevR and NarL, interact in vivo and co-regulate gene expression during aerobic nitrate metabolism

Vandana Malhotra; Ruchi Agrawal; Tammi R. Duncan; Deepak K. Saini; Josephine E. Clark-Curtiss

doi:10.1074/jbc.M114.591800

Mycobacterium tuberculosis response regulators, DevR and NarL, interact in vivo and co-regulate gene expression during aerobic nitrate metabolism

Vandana Malhotra, Ruchi Agrawal, Tammi R. Duncan, Deepak K. Saini, Josephine E. Clark-Curtiss

Life Sciences, School of (SOLS)

Research output: Contribution to journal › Article › peer-review

27 Scopus citations

Abstract

Background: M. tuberculosis two-component systems converge to co-regulate gene expression during nitrate metabolism. Results: Analysis of nitrate/nitrite-responsive gene expression reveals novel interaction between NarL and DevR response regulators. Conclusion: Cooperative binding of NarL and DevR enables co-regulation of a subset of genes. Significance: The findings establish endogenous nitrite as a signal and highlight interplay between M. tuberculosis NarLS and DevRS/DosT signaling systems.

Original language	English (US)
Pages (from-to)	8294-8309
Number of pages	16
Journal	Journal of Biological Chemistry
Volume	290
Issue number	13
DOIs	https://doi.org/10.1074/jbc.M114.591800
State	Published - Mar 27 2015

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Cell Biology

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title = "Mycobacterium tuberculosis response regulators, DevR and NarL, interact in vivo and co-regulate gene expression during aerobic nitrate metabolism",

abstract = "Background: M. tuberculosis two-component systems converge to co-regulate gene expression during nitrate metabolism. Results: Analysis of nitrate/nitrite-responsive gene expression reveals novel interaction between NarL and DevR response regulators. Conclusion: Cooperative binding of NarL and DevR enables co-regulation of a subset of genes. Significance: The findings establish endogenous nitrite as a signal and highlight interplay between M. tuberculosis NarLS and DevRS/DosT signaling systems.",

author = "Vandana Malhotra and Ruchi Agrawal and Duncan, {Tammi R.} and Saini, {Deepak K.} and Clark-Curtiss, {Josephine E.}",

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AU - Malhotra, Vandana

AU - Agrawal, Ruchi

AU - Duncan, Tammi R.

AU - Saini, Deepak K.

AU - Clark-Curtiss, Josephine E.

PY - 2015/3/27

Y1 - 2015/3/27

N2 - Background: M. tuberculosis two-component systems converge to co-regulate gene expression during nitrate metabolism. Results: Analysis of nitrate/nitrite-responsive gene expression reveals novel interaction between NarL and DevR response regulators. Conclusion: Cooperative binding of NarL and DevR enables co-regulation of a subset of genes. Significance: The findings establish endogenous nitrite as a signal and highlight interplay between M. tuberculosis NarLS and DevRS/DosT signaling systems.

AB - Background: M. tuberculosis two-component systems converge to co-regulate gene expression during nitrate metabolism. Results: Analysis of nitrate/nitrite-responsive gene expression reveals novel interaction between NarL and DevR response regulators. Conclusion: Cooperative binding of NarL and DevR enables co-regulation of a subset of genes. Significance: The findings establish endogenous nitrite as a signal and highlight interplay between M. tuberculosis NarLS and DevRS/DosT signaling systems.

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Mycobacterium tuberculosis response regulators, DevR and NarL, interact in vivo and co-regulate gene expression during aerobic nitrate metabolism

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