Mutational analysis of roles for extracellular cysteine residues in the assembly and function of human α7-nicotinic acetylcholine receptors

Travis Dunckley, J. Wu, L. Zhao, R. J. Lukas

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Nicotinic acetylcholine receptors (nAChR) containing α7 subunits self-assemble into simple, homopentameric complexes. However, successful heterologous expression of functional α7-nAChR has only been achieved in a few host cell types, such as the SH-EP1 human epithelial cell line. All ionotropic glycine receptor, GABAA receptor, 5-HT3 receptor, and nAChR subunits contain a pair of highly conserved cysteine residues (C150 and C164 for α7 subunits) in their N-terminal extracellular domain. These residues are thought to be involved in the formation of a conserved cystine loop that is critical to the proper folding and assembly of subunits. However, nAChR α7 (and α8) subunits also contain a third cysteine residue, C138, N-terminal to the conserved cysteine pair. Using SH-EP1 cells as a host for heterologous expression, we evaluated the roles of C138, C150, and C164 in subunit folding, assembly, and cell surface expression and function of α7-nAChR. Results indicate that mutation of C138, but not of C150 or C164, yields an nAChR that can assemble to form 125I-labeled α-bungarotoxin binding sites expressed on the cell surface. Further, whole-cell patch clamp recordings demonstrate that mutation of C138 to alanine does not alter the function of the fully assembled α7-nAChR. These results indicate that C150 and C164 are required for surface expression, but that C138 is neither necessary for nor inhibitory toward the surface expression and function of human α7-nAChR. These results suggest that disulfide bond formation between C138 and either C150 or C164, if it occurs, has no significant effect on α7-nAChR assembly or function.

Original languageEnglish (US)
Pages (from-to)870-876
Number of pages7
JournalBiochemistry
Volume42
Issue number4
DOIs
StatePublished - Feb 4 2003
Externally publishedYes

Fingerprint

Nicotinic Receptors
Cysteine
Glycine Receptors
Receptors, Serotonin, 5-HT3
Bungarotoxins
Mutation
Cystine
Clamping devices
GABA-A Receptors
Disulfides
Alanine
Epithelial Cells
Binding Sites
Cell Line

ASJC Scopus subject areas

  • Biochemistry

Cite this

Mutational analysis of roles for extracellular cysteine residues in the assembly and function of human α7-nicotinic acetylcholine receptors. / Dunckley, Travis; Wu, J.; Zhao, L.; Lukas, R. J.

In: Biochemistry, Vol. 42, No. 4, 04.02.2003, p. 870-876.

Research output: Contribution to journalArticle

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