Muscle-specific myosin heavy chain shifts in response to a long-term high fat/high sugar diet and resveratrol treatment in nonhuman primates

Jon-Philippe Hyatt, Lisa Nguyen, Allison E. Hall, Ashley M. Huber, Jessica C. Kocan, Julie A. Mattison, Rafael de Cabo, Jeannine R. LaRocque, Robert J. Talmadge

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

Shifts in myosin heavy chain (MHC) expression within skeletal muscle can be induced by a host of stimuli including, but not limited to, physical activity, alterations in neural activity, aging, and diet or obesity. Here, we hypothesized that both age and a long-term (2 year) high fat/high sugar diet (HFS) would induce a slow to fast MHC shift within the plantaris, soleus, and extensor digitorum longus (EDL) muscles from rhesus monkeys. Furthermore, we tested whether supplementation with resveratrol, a naturally occurring compound that has been attributed with augmenting aerobic potential through mitochondrial proliferation, would counteract any diet-induced MHC changes by promoting a fast to slow isoform switch. In general, we found that MHC isoforms were not altered by aging during mid-life. The HFS diet had the largest impact within the soleus muscle where the greatest slow to fast isoform shifts were observed in both mRNA and protein indicators. As expected, long-term resveratrol treatment counteracted, or blunted, these diet-induced shifts within the soleus muscle. The plantaris muscle also demonstrated a fast-to-slow phenotypic response to resveratrol treatment. In conclusion, diet or resveratrol treatment impacts skeletal muscle phenotype in a muscle-specific manner and resveratrol supplementation may be one approach for promoting the fatigue-resistant MHC (type I) isoform especially if its expression is blunted as a result of a long-term high fat/sugar diet.

Original languageEnglish (US)
Article number77
JournalFrontiers in Physiology
Volume7
Issue numberMAR
DOIs
StatePublished - Mar 2 2016
Externally publishedYes

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Keywords

  • GLUT4
  • MHC
  • PGC-1α
  • Rhesus macaque
  • Skeletal muscle

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

Cite this

Hyatt, J-P., Nguyen, L., Hall, A. E., Huber, A. M., Kocan, J. C., Mattison, J. A., de Cabo, R., LaRocque, J. R., & Talmadge, R. J. (2016). Muscle-specific myosin heavy chain shifts in response to a long-term high fat/high sugar diet and resveratrol treatment in nonhuman primates. Frontiers in Physiology, 7(MAR), [77]. https://doi.org/10.3389/fphys.2016.00077