Muscle-specific myosin heavy chain shifts in response to a long-term high fat/high sugar diet and resveratrol treatment in nonhuman primates

Jon-Philippe Hyatt, Lisa Nguyen, Allison E. Hall, Ashley M. Huber, Jessica C. Kocan, Julie A. Mattison, Rafael de Cabo, Jeannine R. LaRocque, Robert J. Talmadge

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Shifts in myosin heavy chain (MHC) expression within skeletal muscle can be induced by a host of stimuli including, but not limited to, physical activity, alterations in neural activity, aging, and diet or obesity. Here, we hypothesized that both age and a long-term (2 year) high fat/high sugar diet (HFS) would induce a slow to fast MHC shift within the plantaris, soleus, and extensor digitorum longus (EDL) muscles from rhesus monkeys. Furthermore, we tested whether supplementation with resveratrol, a naturally occurring compound that has been attributed with augmenting aerobic potential through mitochondrial proliferation, would counteract any diet-induced MHC changes by promoting a fast to slow isoform switch. In general, we found that MHC isoforms were not altered by aging during mid-life. The HFS diet had the largest impact within the soleus muscle where the greatest slow to fast isoform shifts were observed in both mRNA and protein indicators. As expected, long-term resveratrol treatment counteracted, or blunted, these diet-induced shifts within the soleus muscle. The plantaris muscle also demonstrated a fast-to-slow phenotypic response to resveratrol treatment. In conclusion, diet or resveratrol treatment impacts skeletal muscle phenotype in a muscle-specific manner and resveratrol supplementation may be one approach for promoting the fatigue-resistant MHC (type I) isoform especially if its expression is blunted as a result of a long-term high fat/sugar diet.

Original languageEnglish (US)
Article number77
JournalFrontiers in Physiology
Volume7
Issue numberMAR
DOIs
StatePublished - Mar 2 2016
Externally publishedYes

Fingerprint

Myosin Heavy Chains
High Fat Diet
Primates
Skeletal Muscle
Diet
Protein Isoforms
Muscles
Macaca mulatta
Fatigue
Obesity
resveratrol
Phenotype
Messenger RNA
Proteins

Keywords

  • GLUT4
  • MHC
  • PGC-1α
  • Rhesus macaque
  • Skeletal muscle

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

Cite this

Muscle-specific myosin heavy chain shifts in response to a long-term high fat/high sugar diet and resveratrol treatment in nonhuman primates. / Hyatt, Jon-Philippe; Nguyen, Lisa; Hall, Allison E.; Huber, Ashley M.; Kocan, Jessica C.; Mattison, Julie A.; de Cabo, Rafael; LaRocque, Jeannine R.; Talmadge, Robert J.

In: Frontiers in Physiology, Vol. 7, No. MAR, 77, 02.03.2016.

Research output: Contribution to journalArticle

Hyatt, Jon-Philippe ; Nguyen, Lisa ; Hall, Allison E. ; Huber, Ashley M. ; Kocan, Jessica C. ; Mattison, Julie A. ; de Cabo, Rafael ; LaRocque, Jeannine R. ; Talmadge, Robert J. / Muscle-specific myosin heavy chain shifts in response to a long-term high fat/high sugar diet and resveratrol treatment in nonhuman primates. In: Frontiers in Physiology. 2016 ; Vol. 7, No. MAR.
@article{28c1d6eb55654b0b96a8878f2f4a5d37,
title = "Muscle-specific myosin heavy chain shifts in response to a long-term high fat/high sugar diet and resveratrol treatment in nonhuman primates",
abstract = "Shifts in myosin heavy chain (MHC) expression within skeletal muscle can be induced by a host of stimuli including, but not limited to, physical activity, alterations in neural activity, aging, and diet or obesity. Here, we hypothesized that both age and a long-term (2 year) high fat/high sugar diet (HFS) would induce a slow to fast MHC shift within the plantaris, soleus, and extensor digitorum longus (EDL) muscles from rhesus monkeys. Furthermore, we tested whether supplementation with resveratrol, a naturally occurring compound that has been attributed with augmenting aerobic potential through mitochondrial proliferation, would counteract any diet-induced MHC changes by promoting a fast to slow isoform switch. In general, we found that MHC isoforms were not altered by aging during mid-life. The HFS diet had the largest impact within the soleus muscle where the greatest slow to fast isoform shifts were observed in both mRNA and protein indicators. As expected, long-term resveratrol treatment counteracted, or blunted, these diet-induced shifts within the soleus muscle. The plantaris muscle also demonstrated a fast-to-slow phenotypic response to resveratrol treatment. In conclusion, diet or resveratrol treatment impacts skeletal muscle phenotype in a muscle-specific manner and resveratrol supplementation may be one approach for promoting the fatigue-resistant MHC (type I) isoform especially if its expression is blunted as a result of a long-term high fat/sugar diet.",
keywords = "GLUT4, MHC, PGC-1α, Rhesus macaque, Skeletal muscle",
author = "Jon-Philippe Hyatt and Lisa Nguyen and Hall, {Allison E.} and Huber, {Ashley M.} and Kocan, {Jessica C.} and Mattison, {Julie A.} and {de Cabo}, Rafael and LaRocque, {Jeannine R.} and Talmadge, {Robert J.}",
year = "2016",
month = "3",
day = "2",
doi = "10.3389/fphys.2016.00077",
language = "English (US)",
volume = "7",
journal = "Frontiers in Physiology",
issn = "1664-042X",
publisher = "Frontiers Research Foundation",
number = "MAR",

}

TY - JOUR

T1 - Muscle-specific myosin heavy chain shifts in response to a long-term high fat/high sugar diet and resveratrol treatment in nonhuman primates

AU - Hyatt, Jon-Philippe

AU - Nguyen, Lisa

AU - Hall, Allison E.

AU - Huber, Ashley M.

AU - Kocan, Jessica C.

AU - Mattison, Julie A.

AU - de Cabo, Rafael

AU - LaRocque, Jeannine R.

AU - Talmadge, Robert J.

PY - 2016/3/2

Y1 - 2016/3/2

N2 - Shifts in myosin heavy chain (MHC) expression within skeletal muscle can be induced by a host of stimuli including, but not limited to, physical activity, alterations in neural activity, aging, and diet or obesity. Here, we hypothesized that both age and a long-term (2 year) high fat/high sugar diet (HFS) would induce a slow to fast MHC shift within the plantaris, soleus, and extensor digitorum longus (EDL) muscles from rhesus monkeys. Furthermore, we tested whether supplementation with resveratrol, a naturally occurring compound that has been attributed with augmenting aerobic potential through mitochondrial proliferation, would counteract any diet-induced MHC changes by promoting a fast to slow isoform switch. In general, we found that MHC isoforms were not altered by aging during mid-life. The HFS diet had the largest impact within the soleus muscle where the greatest slow to fast isoform shifts were observed in both mRNA and protein indicators. As expected, long-term resveratrol treatment counteracted, or blunted, these diet-induced shifts within the soleus muscle. The plantaris muscle also demonstrated a fast-to-slow phenotypic response to resveratrol treatment. In conclusion, diet or resveratrol treatment impacts skeletal muscle phenotype in a muscle-specific manner and resveratrol supplementation may be one approach for promoting the fatigue-resistant MHC (type I) isoform especially if its expression is blunted as a result of a long-term high fat/sugar diet.

AB - Shifts in myosin heavy chain (MHC) expression within skeletal muscle can be induced by a host of stimuli including, but not limited to, physical activity, alterations in neural activity, aging, and diet or obesity. Here, we hypothesized that both age and a long-term (2 year) high fat/high sugar diet (HFS) would induce a slow to fast MHC shift within the plantaris, soleus, and extensor digitorum longus (EDL) muscles from rhesus monkeys. Furthermore, we tested whether supplementation with resveratrol, a naturally occurring compound that has been attributed with augmenting aerobic potential through mitochondrial proliferation, would counteract any diet-induced MHC changes by promoting a fast to slow isoform switch. In general, we found that MHC isoforms were not altered by aging during mid-life. The HFS diet had the largest impact within the soleus muscle where the greatest slow to fast isoform shifts were observed in both mRNA and protein indicators. As expected, long-term resveratrol treatment counteracted, or blunted, these diet-induced shifts within the soleus muscle. The plantaris muscle also demonstrated a fast-to-slow phenotypic response to resveratrol treatment. In conclusion, diet or resveratrol treatment impacts skeletal muscle phenotype in a muscle-specific manner and resveratrol supplementation may be one approach for promoting the fatigue-resistant MHC (type I) isoform especially if its expression is blunted as a result of a long-term high fat/sugar diet.

KW - GLUT4

KW - MHC

KW - PGC-1α

KW - Rhesus macaque

KW - Skeletal muscle

UR - http://www.scopus.com/inward/record.url?scp=84964607532&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84964607532&partnerID=8YFLogxK

U2 - 10.3389/fphys.2016.00077

DO - 10.3389/fphys.2016.00077

M3 - Article

VL - 7

JO - Frontiers in Physiology

JF - Frontiers in Physiology

SN - 1664-042X

IS - MAR

M1 - 77

ER -