TY - JOUR
T1 - Muscle-specific myosin heavy chain shifts in response to a long-term high fat/high sugar diet and resveratrol treatment in nonhuman primates
AU - Hyatt, Jon Philippe K.
AU - Nguyen, Lisa
AU - Hall, Allison E.
AU - Huber, Ashley M.
AU - Kocan, Jessica C.
AU - Mattison, Julie A.
AU - de Cabo, Rafael
AU - LaRocque, Jeannine R.
AU - Talmadge, Robert J.
N1 - Funding Information:
The authors would like to thank Fadia Haddad for helpful insight on myosin heavy chain primers construction and Elisa Jayne Bienenstock for statistical work and consultation. Myosin heavy chain antibodies were generously supplied by S. Schiaffino (University of Padova, Padova, Italy). This study was supported in part by the National Institute on Aging Intramural Research Program, NIH, and the Sigma Xi Society Grants-in-Aid Research Program for undergraduate student researchers.
Publisher Copyright:
© 2016 Hyatt, Nguyen, Hall, Huber, Kocan, Mattison, de Cabo, LaRocque and Talmadge.
PY - 2016/3/2
Y1 - 2016/3/2
N2 - Shifts in myosin heavy chain (MHC) expression within skeletal muscle can be induced by a host of stimuli including, but not limited to, physical activity, alterations in neural activity, aging, and diet or obesity. Here, we hypothesized that both age and a long-term (2 year) high fat/high sugar diet (HFS) would induce a slow to fast MHC shift within the plantaris, soleus, and extensor digitorum longus (EDL) muscles from rhesus monkeys. Furthermore, we tested whether supplementation with resveratrol, a naturally occurring compound that has been attributed with augmenting aerobic potential through mitochondrial proliferation, would counteract any diet-induced MHC changes by promoting a fast to slow isoform switch. In general, we found that MHC isoforms were not altered by aging during mid-life. The HFS diet had the largest impact within the soleus muscle where the greatest slow to fast isoform shifts were observed in both mRNA and protein indicators. As expected, long-term resveratrol treatment counteracted, or blunted, these diet-induced shifts within the soleus muscle. The plantaris muscle also demonstrated a fast-to-slow phenotypic response to resveratrol treatment. In conclusion, diet or resveratrol treatment impacts skeletal muscle phenotype in a muscle-specific manner and resveratrol supplementation may be one approach for promoting the fatigue-resistant MHC (type I) isoform especially if its expression is blunted as a result of a long-term high fat/sugar diet.
AB - Shifts in myosin heavy chain (MHC) expression within skeletal muscle can be induced by a host of stimuli including, but not limited to, physical activity, alterations in neural activity, aging, and diet or obesity. Here, we hypothesized that both age and a long-term (2 year) high fat/high sugar diet (HFS) would induce a slow to fast MHC shift within the plantaris, soleus, and extensor digitorum longus (EDL) muscles from rhesus monkeys. Furthermore, we tested whether supplementation with resveratrol, a naturally occurring compound that has been attributed with augmenting aerobic potential through mitochondrial proliferation, would counteract any diet-induced MHC changes by promoting a fast to slow isoform switch. In general, we found that MHC isoforms were not altered by aging during mid-life. The HFS diet had the largest impact within the soleus muscle where the greatest slow to fast isoform shifts were observed in both mRNA and protein indicators. As expected, long-term resveratrol treatment counteracted, or blunted, these diet-induced shifts within the soleus muscle. The plantaris muscle also demonstrated a fast-to-slow phenotypic response to resveratrol treatment. In conclusion, diet or resveratrol treatment impacts skeletal muscle phenotype in a muscle-specific manner and resveratrol supplementation may be one approach for promoting the fatigue-resistant MHC (type I) isoform especially if its expression is blunted as a result of a long-term high fat/sugar diet.
KW - GLUT4
KW - MHC
KW - PGC-1α
KW - Rhesus macaque
KW - Skeletal muscle
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U2 - 10.3389/fphys.2016.00077
DO - 10.3389/fphys.2016.00077
M3 - Article
AN - SCOPUS:84964607532
SN - 1664-042X
VL - 7
JO - Frontiers in Physiology
JF - Frontiers in Physiology
IS - MAR
M1 - 77
ER -