Multitoxin biosensor-mass spectrometry analysis

A new approach for rapid, real-time, sensitive analysis of staphylococcal toxins in food

Dobrin Nedelkov, Avraham Rasooly, Randall W. Nelson

Research output: Contribution to journalArticle

96 Citations (Scopus)

Abstract

Biomolecular interaction analysis mass spectrometry (BIA-MS) was applied to detection of bacterial toxins in food samples. This two-step approach utilizes surface plasmon resonance (SPR) to detect the binding of the toxin(s) to antibodies immobilized on a surface of a sensor chip. SPR detection is then followed by identification of the bound toxin(s) by matrix- assisted laser desorption/ionization time-of-flight mass spectrometry. Staphylococcal enterotoxin B (SEB) was readily detected in milk and mushroom samples at levels of 1 ng/ml. In addition, non-specific binding of food components to the immobilized antibody and to the sensor chip surface was detected. To evaluate the applicability of BIA-MS in the analysis of materials containing multiple toxic components, sample containing both SEB and toxic-shock syndrome toxin-1 was analyzed. Both toxins were successfully and simultaneously detected through the utilization of multiaffinity sensor chip surfaces. (C) 2000 Elsevier Science B.V.

Original languageEnglish (US)
Pages (from-to)1-13
Number of pages13
JournalInternational Journal of Food Microbiology
Volume60
Issue number1
DOIs
StatePublished - Sep 15 2000

Fingerprint

biosensors
Biosensing Techniques
Immobilized Antibodies
Mass Spectrometry
toxins
Surface Plasmon Resonance
mass spectrometry
sensors (equipment)
surface plasmon resonance
Food
enterotoxins
Bacterial Toxins
Poisons
Agaricales
bacterial toxins
septic shock
antibodies
matrix-assisted laser desorption-ionization mass spectrometry
Milk
Lasers

Keywords

  • Biomolecular interaction analysis mass spectrometry
  • Enterotoxin B
  • MALDI-TOF mass spectrometry
  • Staphylococcus aureus
  • Surface plasmon resonance
  • Toxic-shock syndrome toxin-1

ASJC Scopus subject areas

  • Food Science
  • Microbiology

Cite this

Multitoxin biosensor-mass spectrometry analysis : A new approach for rapid, real-time, sensitive analysis of staphylococcal toxins in food. / Nedelkov, Dobrin; Rasooly, Avraham; Nelson, Randall W.

In: International Journal of Food Microbiology, Vol. 60, No. 1, 15.09.2000, p. 1-13.

Research output: Contribution to journalArticle

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