Abstract
Biomolecular interaction analysis mass spectrometry (BIA-MS) was applied to detection of bacterial toxins in food samples. This two-step approach utilizes surface plasmon resonance (SPR) to detect the binding of the toxin(s) to antibodies immobilized on a surface of a sensor chip. SPR detection is then followed by identification of the bound toxin(s) by matrix- assisted laser desorption/ionization time-of-flight mass spectrometry. Staphylococcal enterotoxin B (SEB) was readily detected in milk and mushroom samples at levels of 1 ng/ml. In addition, non-specific binding of food components to the immobilized antibody and to the sensor chip surface was detected. To evaluate the applicability of BIA-MS in the analysis of materials containing multiple toxic components, sample containing both SEB and toxic-shock syndrome toxin-1 was analyzed. Both toxins were successfully and simultaneously detected through the utilization of multiaffinity sensor chip surfaces. (C) 2000 Elsevier Science B.V.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1-13 |
| Number of pages | 13 |
| Journal | International Journal of Food Microbiology |
| Volume | 60 |
| Issue number | 1 |
| DOIs | |
| State | Published - Sep 15 2000 |
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Keywords
- Biomolecular interaction analysis mass spectrometry
- Enterotoxin B
- MALDI-TOF mass spectrometry
- Staphylococcus aureus
- Surface plasmon resonance
- Toxic-shock syndrome toxin-1
ASJC Scopus subject areas
- Food Science
- Microbiology
Cite this
Multitoxin biosensor-mass spectrometry analysis : A new approach for rapid, real-time, sensitive analysis of staphylococcal toxins in food. / Nedelkov, Dobrin; Rasooly, Avraham; Nelson, Randall W.
In: International Journal of Food Microbiology, Vol. 60, No. 1, 15.09.2000, p. 1-13.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Multitoxin biosensor-mass spectrometry analysis
T2 - A new approach for rapid, real-time, sensitive analysis of staphylococcal toxins in food
AU - Nedelkov, Dobrin
AU - Rasooly, Avraham
AU - Nelson, Randall W.
PY - 2000/9/15
Y1 - 2000/9/15
N2 - Biomolecular interaction analysis mass spectrometry (BIA-MS) was applied to detection of bacterial toxins in food samples. This two-step approach utilizes surface plasmon resonance (SPR) to detect the binding of the toxin(s) to antibodies immobilized on a surface of a sensor chip. SPR detection is then followed by identification of the bound toxin(s) by matrix- assisted laser desorption/ionization time-of-flight mass spectrometry. Staphylococcal enterotoxin B (SEB) was readily detected in milk and mushroom samples at levels of 1 ng/ml. In addition, non-specific binding of food components to the immobilized antibody and to the sensor chip surface was detected. To evaluate the applicability of BIA-MS in the analysis of materials containing multiple toxic components, sample containing both SEB and toxic-shock syndrome toxin-1 was analyzed. Both toxins were successfully and simultaneously detected through the utilization of multiaffinity sensor chip surfaces. (C) 2000 Elsevier Science B.V.
AB - Biomolecular interaction analysis mass spectrometry (BIA-MS) was applied to detection of bacterial toxins in food samples. This two-step approach utilizes surface plasmon resonance (SPR) to detect the binding of the toxin(s) to antibodies immobilized on a surface of a sensor chip. SPR detection is then followed by identification of the bound toxin(s) by matrix- assisted laser desorption/ionization time-of-flight mass spectrometry. Staphylococcal enterotoxin B (SEB) was readily detected in milk and mushroom samples at levels of 1 ng/ml. In addition, non-specific binding of food components to the immobilized antibody and to the sensor chip surface was detected. To evaluate the applicability of BIA-MS in the analysis of materials containing multiple toxic components, sample containing both SEB and toxic-shock syndrome toxin-1 was analyzed. Both toxins were successfully and simultaneously detected through the utilization of multiaffinity sensor chip surfaces. (C) 2000 Elsevier Science B.V.
KW - Biomolecular interaction analysis mass spectrometry
KW - Enterotoxin B
KW - MALDI-TOF mass spectrometry
KW - Staphylococcus aureus
KW - Surface plasmon resonance
KW - Toxic-shock syndrome toxin-1
UR - http://www.scopus.com/inward/record.url?scp=0034666508&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034666508&partnerID=8YFLogxK
U2 - 10.1016/S0168-1605(00)00328-7
DO - 10.1016/S0168-1605(00)00328-7
M3 - Article
C2 - 11014517
AN - SCOPUS:0034666508
VL - 60
SP - 1
EP - 13
JO - International Journal of Food Microbiology
JF - International Journal of Food Microbiology
SN - 0168-1605
IS - 1
ER -