Multitoxin biosensor-mass spectrometry analysis: A new approach for rapid, real-time, sensitive analysis of staphylococcal toxins in food

Dobrin Nedelkov, Avraham Rasooly, Randall W. Nelson

Research output: Contribution to journalArticle

96 Scopus citations

Abstract

Biomolecular interaction analysis mass spectrometry (BIA-MS) was applied to detection of bacterial toxins in food samples. This two-step approach utilizes surface plasmon resonance (SPR) to detect the binding of the toxin(s) to antibodies immobilized on a surface of a sensor chip. SPR detection is then followed by identification of the bound toxin(s) by matrix- assisted laser desorption/ionization time-of-flight mass spectrometry. Staphylococcal enterotoxin B (SEB) was readily detected in milk and mushroom samples at levels of 1 ng/ml. In addition, non-specific binding of food components to the immobilized antibody and to the sensor chip surface was detected. To evaluate the applicability of BIA-MS in the analysis of materials containing multiple toxic components, sample containing both SEB and toxic-shock syndrome toxin-1 was analyzed. Both toxins were successfully and simultaneously detected through the utilization of multiaffinity sensor chip surfaces. (C) 2000 Elsevier Science B.V.

Original languageEnglish (US)
Pages (from-to)1-13
Number of pages13
JournalInternational Journal of Food Microbiology
Volume60
Issue number1
DOIs
StatePublished - Sep 15 2000

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Keywords

  • Biomolecular interaction analysis mass spectrometry
  • Enterotoxin B
  • MALDI-TOF mass spectrometry
  • Staphylococcus aureus
  • Surface plasmon resonance
  • Toxic-shock syndrome toxin-1

ASJC Scopus subject areas

  • Food Science
  • Microbiology

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