Abstract
Reported here is the development of a multiplexed mass spectrometric immunoassay (MSIA) for the detection of myocardial infarction (MI). The assay is the product of a study that systematically progresses from biomarker discovery-to identification and verification-to assay design, data analysis, and statistical challenge. During targeted population proteomics investigations, two novel biomarkers, serum amyloid A1α and S-sulfated transthyretin, were found to be responsive to MI. These putative markers were subsequently screened in larger cohorts of individuals to verify their responsiveness toward MI. Upon verification, a multiplexed assay was designed that was capable of simultaneously monitoring the new markers plus a previously established MI-marker (myoglobin). The multiplexed MSIA was applied to two 96-sample sets comprised of 48-MI/48-healthy and 19-MI/77-healthy, which served as training and case cohorts, respectively. Data evaluation using either preset reference levels or multivariate analysis exhibited sensitivities and specificities of >97%. These findings illustrate the importance of using systematic approaches in clinical proteomics to discover biomarkers and produce high-performance assays relevant to disease.
Original language | English (US) |
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Pages (from-to) | 2928-2934 |
Number of pages | 7 |
Journal | Journal of Proteome Research |
Volume | 5 |
Issue number | 11 |
DOIs | |
State | Published - Nov 2006 |
Keywords
- Human plasma
- Immunoassay
- Mass spectrometry
- Multiplexing
- Myocardial infarction
ASJC Scopus subject areas
- Biochemistry
- General Chemistry