Multiplexed mass spectrometric immunoassay in biomarker research: A novel approach to the determination of a myocardial infarct

Urban A. Kiernan, Dobrin Nedelkov, Randall W. Nelson

Research output: Contribution to journalArticle

43 Scopus citations

Abstract

Reported here is the development of a multiplexed mass spectrometric immunoassay (MSIA) for the detection of myocardial infarction (MI). The assay is the product of a study that systematically progresses from biomarker discovery-to identification and verification-to assay design, data analysis, and statistical challenge. During targeted population proteomics investigations, two novel biomarkers, serum amyloid A1α and S-sulfated transthyretin, were found to be responsive to MI. These putative markers were subsequently screened in larger cohorts of individuals to verify their responsiveness toward MI. Upon verification, a multiplexed assay was designed that was capable of simultaneously monitoring the new markers plus a previously established MI-marker (myoglobin). The multiplexed MSIA was applied to two 96-sample sets comprised of 48-MI/48-healthy and 19-MI/77-healthy, which served as training and case cohorts, respectively. Data evaluation using either preset reference levels or multivariate analysis exhibited sensitivities and specificities of >97%. These findings illustrate the importance of using systematic approaches in clinical proteomics to discover biomarkers and produce high-performance assays relevant to disease.

Original languageEnglish (US)
Pages (from-to)2928-2934
Number of pages7
JournalJournal of Proteome Research
Volume5
Issue number11
DOIs
StatePublished - Nov 2006

Keywords

  • Human plasma
  • Immunoassay
  • Mass spectrometry
  • Multiplexing
  • Myocardial infarction

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)

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