Multiple sensor arrays for single cell metabolic analysis

Ganquan Song; Rishabh M. Shetty; Haixin Zhu; Shashanka Ashili; Liqiang Zhang; Grace Kim; Andrew Shabilla; Wacey Teller; Qian Mei; Laimonas Kelbauskas; Yanqing Tian; Hong Wang; Roger H. Johnson; Deirdre Meldrum

doi:10.1109/ICSENS.2013.6688453

Multiple sensor arrays for single cell metabolic analysis

Ganquan Song, Rishabh M. Shetty, Haixin Zhu, Shashanka Ashili, Liqiang Zhang, Grace Kim, Andrew Shabilla, Wacey Teller, Qian Mei, Laimonas Kelbauskas, Yanqing Tian, Hong Wang, Roger H. Johnson, Deirdre Meldrum

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

1 Scopus citations

Abstract

We present the design, fabrication and characterization of multiple micro-pocket lid arrays used in live single cell metabolic analysis. In previous work we reported a platform for quantifying single cell oxygen consumption rates realized using a fused silica deep wet etching process. Here we extend that work to a dual-depth wet etching process for microfabrication of multiple sensor trapping (MST) lid arrays. Each lid comprises multiple micro-pockets. Oxygen, pH, other extra-cellular sensors, and reference dye were deposited in the pockets. In order to achieve simultaneous monitoring of multiple metabolic parameters, the lid array serves to hermetically seal arrays of microwells, each containing a single cell. The dual-depth etching process we developed can be easily applied to other glass-based microfabrication purposes requiring dual- or multiple-depth microstructures.

Original language	English (US)
Title of host publication	IEEE SENSORS 2013 - Proceedings
Publisher	IEEE Computer Society
ISBN (Print)	9781467346405
DOIs	https://doi.org/10.1109/ICSENS.2013.6688453
State	Published - Jan 1 2013
Event	12th IEEE SENSORS 2013 Conference - Baltimore, MD, United States Duration: Nov 4 2013 → Nov 6 2013

Publication series

Name	Proceedings of IEEE Sensors

Other

Other	12th IEEE SENSORS 2013 Conference
Country/Territory	United States
City	Baltimore, MD
Period	11/4/13 → 11/6/13

ASJC Scopus subject areas

Electrical and Electronic Engineering

Access to Document

10.1109/ICSENS.2013.6688453

Cite this

Song, G., Shetty, R. M., Zhu, H., Ashili, S., Zhang, L., Kim, G., Shabilla, A., Teller, W., Mei, Q., Kelbauskas, L., Tian, Y., Wang, H., Johnson, R. H., & Meldrum, D. (2013). Multiple sensor arrays for single cell metabolic analysis. In IEEE SENSORS 2013 - Proceedings Article 6688453 (Proceedings of IEEE Sensors). IEEE Computer Society. https://doi.org/10.1109/ICSENS.2013.6688453

Song, G, Shetty, RM, Zhu, H, Ashili, S, Zhang, L, Kim, G, Shabilla, A, Teller, W, Mei, Q, Kelbauskas, L, Tian, Y, Wang, H, Johnson, RH & Meldrum, D 2013, Multiple sensor arrays for single cell metabolic analysis. in IEEE SENSORS 2013 - Proceedings., 6688453, Proceedings of IEEE Sensors, IEEE Computer Society, 12th IEEE SENSORS 2013 Conference, Baltimore, MD, United States, 11/4/13. https://doi.org/10.1109/ICSENS.2013.6688453

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title = "Multiple sensor arrays for single cell metabolic analysis",

abstract = "We present the design, fabrication and characterization of multiple micro-pocket lid arrays used in live single cell metabolic analysis. In previous work we reported a platform for quantifying single cell oxygen consumption rates realized using a fused silica deep wet etching process. Here we extend that work to a dual-depth wet etching process for microfabrication of multiple sensor trapping (MST) lid arrays. Each lid comprises multiple micro-pockets. Oxygen, pH, other extra-cellular sensors, and reference dye were deposited in the pockets. In order to achieve simultaneous monitoring of multiple metabolic parameters, the lid array serves to hermetically seal arrays of microwells, each containing a single cell. The dual-depth etching process we developed can be easily applied to other glass-based microfabrication purposes requiring dual- or multiple-depth microstructures.",

author = "Ganquan Song and Shetty, {Rishabh M.} and Haixin Zhu and Shashanka Ashili and Liqiang Zhang and Grace Kim and Andrew Shabilla and Wacey Teller and Qian Mei and Laimonas Kelbauskas and Yanqing Tian and Hong Wang and Johnson, {Roger H.} and Deirdre Meldrum",

year = "2013",

month = jan,

day = "1",

doi = "10.1109/ICSENS.2013.6688453",

language = "English (US)",

isbn = "9781467346405",

series = "Proceedings of IEEE Sensors",

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booktitle = "IEEE SENSORS 2013 - Proceedings",

note = "12th IEEE SENSORS 2013 Conference ; Conference date: 04-11-2013 Through 06-11-2013",

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AU - Song, Ganquan

AU - Shetty, Rishabh M.

AU - Zhu, Haixin

AU - Ashili, Shashanka

AU - Zhang, Liqiang

AU - Kim, Grace

AU - Shabilla, Andrew

AU - Teller, Wacey

AU - Mei, Qian

AU - Kelbauskas, Laimonas

AU - Tian, Yanqing

AU - Wang, Hong

AU - Johnson, Roger H.

AU - Meldrum, Deirdre

PY - 2013/1/1

Y1 - 2013/1/1

N2 - We present the design, fabrication and characterization of multiple micro-pocket lid arrays used in live single cell metabolic analysis. In previous work we reported a platform for quantifying single cell oxygen consumption rates realized using a fused silica deep wet etching process. Here we extend that work to a dual-depth wet etching process for microfabrication of multiple sensor trapping (MST) lid arrays. Each lid comprises multiple micro-pockets. Oxygen, pH, other extra-cellular sensors, and reference dye were deposited in the pockets. In order to achieve simultaneous monitoring of multiple metabolic parameters, the lid array serves to hermetically seal arrays of microwells, each containing a single cell. The dual-depth etching process we developed can be easily applied to other glass-based microfabrication purposes requiring dual- or multiple-depth microstructures.

AB - We present the design, fabrication and characterization of multiple micro-pocket lid arrays used in live single cell metabolic analysis. In previous work we reported a platform for quantifying single cell oxygen consumption rates realized using a fused silica deep wet etching process. Here we extend that work to a dual-depth wet etching process for microfabrication of multiple sensor trapping (MST) lid arrays. Each lid comprises multiple micro-pockets. Oxygen, pH, other extra-cellular sensors, and reference dye were deposited in the pockets. In order to achieve simultaneous monitoring of multiple metabolic parameters, the lid array serves to hermetically seal arrays of microwells, each containing a single cell. The dual-depth etching process we developed can be easily applied to other glass-based microfabrication purposes requiring dual- or multiple-depth microstructures.

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U2 - 10.1109/ICSENS.2013.6688453

DO - 10.1109/ICSENS.2013.6688453

M3 - Conference contribution

AN - SCOPUS:84893984469

SN - 9781467346405

T3 - Proceedings of IEEE Sensors

BT - IEEE SENSORS 2013 - Proceedings

PB - IEEE Computer Society

T2 - 12th IEEE SENSORS 2013 Conference

Y2 - 4 November 2013 through 6 November 2013

ER -

Multiple sensor arrays for single cell metabolic analysis

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