Multiple reductive-dehalogenase-homologous genes are simultaneously transcribed during dechlorination by dehalococcoides-containing cultures

Alison S. Waller, Rosa Krajmalnik-Brown, Frank E. Löffler, Elizabeth A. Edwards

Research output: Contribution to journalArticle

101 Citations (Scopus)

Abstract

Degenerate primers were used to amplify 14 distinct reductive-dehalogenase- homologous (RDH) genes from the Dehalococcoides-containing mixed culture KB1. Most of the corresponding predicted proteins were highly similar (97 to >99% amino acid identity) to previously reported Dehalococcoides reductive dehalogenases. To examine the differential transcription of these RDH genes, KB1 was split into five subcultures amended with either trichloroethene, cis-1,2-dichloroethene, vinyl chloride, 1,2-dichlorethane, or no chlorinated electron acceptor. Total RNA was extracted following the onset of reductive dechlorination, and RDH transcripts were reverse transcribed and amplified using degenerate primers. The results indicate that the transcription of RDH genes requires the presence of a chlorinated electron acceptor, and for all treatments, multiple RDH genes were simultaneously transcribed, with transcripts of two of the genes being present under all four electron-accepting conditions. Two of the transcribed sequences were highly similar to reported vinyl chloride reductase genes, namely, vcrA from Dehalococcoides sp. strain VS and bvcA from Dehalococcoides sp. strain BAV1. These findings suggest that multiple RDH genes are induced by a single chlorinated substrate and that multiple reductive dehalogenases contribute to chloroethene degradation in KB1.

Original languageEnglish (US)
Pages (from-to)8257-8264
Number of pages8
JournalApplied and Environmental Microbiology
Volume71
Issue number12
DOIs
StatePublished - Dec 2005
Externally publishedYes

Fingerprint

Dehalococcoides
dechlorination
gene
Genes
vinyl chloride
genes
Vinyl Chloride
electrons
Electrons
electron
transcription (genetics)
chloride
Trichloroethylene
mixed culture
trichloroethylene
RNA
Oxidoreductases
amino acid
Amino Acids
substrate

ASJC Scopus subject areas

  • Environmental Science(all)
  • Biotechnology
  • Microbiology

Cite this

Multiple reductive-dehalogenase-homologous genes are simultaneously transcribed during dechlorination by dehalococcoides-containing cultures. / Waller, Alison S.; Krajmalnik-Brown, Rosa; Löffler, Frank E.; Edwards, Elizabeth A.

In: Applied and Environmental Microbiology, Vol. 71, No. 12, 12.2005, p. 8257-8264.

Research output: Contribution to journalArticle

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