TY - JOUR
T1 - Multifunctional PHPMA-Derived Polymer for Ratiometric pH Sensing, Fluorescence Imaging, and Magnetic Resonance Imaging
AU - Su, Fengyu
AU - Agarwal, Shubhangi
AU - Pan, Tingting
AU - Qiao, Yuan
AU - Zhang, Liqiang
AU - Shi, Zhengwei
AU - Kong, Xiangxing
AU - Day, Kevin
AU - Chen, Meiwan
AU - Meldrum, Deirdre
AU - Kodibagkar, Vikram
AU - Tian, Yanqing
N1 - Funding Information:
This work was supported by the NIH National Human Genome Research Institute, Centers of Excellence in Genomic Science, grant number 5P50 HG002360 (D.M., PI), NIH Common Fund LINCS program, grant no. 5U01 CA164250 (D.M., PI), NSF CAREER award # 1351992 (V.K., PI), National Science Foundation of China (21574061, Y.T., PI), Shenzhen Fundamental Research Programs (JCYJ20150630145302243, Y.T., PI), and startup funding of Southern University of Science and Technology (grants nos. 2015-FRG-SUSTC1501A-01, 25/ Y01256009, 25/Y01256114, and 25/Y01256214, Y.T., PI).
Funding Information:
This work was supported by the NIH National Human Genome Research Institute, Centers of Excellence in Genomic Science grant number 5P50 HG002360 (D.M., PI), NIH Common Fund LINCS program grant no. 5U01 CA164250 (D.M., PI), NSF CAREER award # 1351992 (V.K. PI), National Science Foundation of China (21574061, Y.T., PI), Shenzhen Fundamental Research Programs (JCYJ20150630145302243, Y.T., PI), and startup funding of Southern University of Science and Technology (grants nos. 2015-FRG-SUSTC1501A-01, 25/Y01256009, 25/Y01256114, and 25/Y01256214 Y.T., PI).
Publisher Copyright:
© 2017 American Chemical Society.
PY - 2018/1/17
Y1 - 2018/1/17
N2 - In this paper, we report synthesis and characterization of a novel multimodality (MRI/fluorescence) probe for pH sensing and imaging. A multifunctional polymer was derived from poly(N-(2-hydroxypropyl)methacrylamide) (PHPMA) and integrated with a naphthalimide-based-ratiometric fluorescence probe and a gadolinium-1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid complex (Gd-DOTA complex). The polymer was characterized using UV-vis absorption spectrophotometry, fluorescence spectrofluorophotometry, magnetic resonance imaging (MRI), and confocal microscopy for optical and MRI-based pH sensing and cellular imaging. In vitro labeling of macrophage J774 and esophageal CP-A cell lines shows the polymer's ability to be internalized in the cells. The transverse relaxation time (T2) of the polymer was observed to be pH-dependent, whereas the spin-lattice relaxation time (T1) was not. The pH probe in the polymer shows a strong fluorescence-based ratiometric pH response with emission window changes, exhibiting blue emission under acidic conditions and green emission under basic conditions, respectively. This study provides new materials with multimodalities for pH sensing and imaging.
AB - In this paper, we report synthesis and characterization of a novel multimodality (MRI/fluorescence) probe for pH sensing and imaging. A multifunctional polymer was derived from poly(N-(2-hydroxypropyl)methacrylamide) (PHPMA) and integrated with a naphthalimide-based-ratiometric fluorescence probe and a gadolinium-1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid complex (Gd-DOTA complex). The polymer was characterized using UV-vis absorption spectrophotometry, fluorescence spectrofluorophotometry, magnetic resonance imaging (MRI), and confocal microscopy for optical and MRI-based pH sensing and cellular imaging. In vitro labeling of macrophage J774 and esophageal CP-A cell lines shows the polymer's ability to be internalized in the cells. The transverse relaxation time (T2) of the polymer was observed to be pH-dependent, whereas the spin-lattice relaxation time (T1) was not. The pH probe in the polymer shows a strong fluorescence-based ratiometric pH response with emission window changes, exhibiting blue emission under acidic conditions and green emission under basic conditions, respectively. This study provides new materials with multimodalities for pH sensing and imaging.
KW - MRI
KW - dual-modality
KW - fluorescence imaging
KW - pH sensor
KW - polymer
KW - ratiometric sensor
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U2 - 10.1021/acsami.7b15796
DO - 10.1021/acsami.7b15796
M3 - Article
C2 - 29210559
AN - SCOPUS:85040723066
SN - 1944-8244
VL - 10
SP - 1556
EP - 1565
JO - ACS Applied Materials and Interfaces
JF - ACS Applied Materials and Interfaces
IS - 2
ER -