Multidimensional high-throughput screening of displacers

Kaushal Rege, Asif Ladiwala, Steven M. Cramer

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

A multidimensional, batch high-throughput screening (MD-HTS) protocol was developed to investigate the effects of various parameters on the selectivity of ion-exchange protein displacement systems. A variety of molecules were screened, and the results were employed to provided insights into the influence of displacer chemistry and concentration, resin chemistry, and mobile-phase salt counterion on the efficacy and selectivity of these nonlinear chromatographic systems. These results open up the possibility of tailoring the selectivity of displacement separations by choosing appropriate combinations of operating conditions using the MD-HTS technique. The screens were also employed for the identification of displacers and conditions for the separation of a challenging protein mixture by selective displacement chromatography. Column displacements were carried out with potential lead compounds identified from the MD-HTS screens, and the results confirmed that selective displacement could indeed be achieved for this model mixture. Furthermore, the results indicated that this approach is particularly useful when the order of elution is not changed, but the inherent selectivity is increased in the presence of the displacer. The results presented in this paper demonstrate the utility of the MD-HTS technique for rapid method development in protein ion-exchange displacement chromatography.

Original languageEnglish (US)
Pages (from-to)6818-6827
Number of pages10
JournalAnalytical Chemistry
Volume77
Issue number21
DOIs
StatePublished - Nov 1 2005
Externally publishedYes

Fingerprint

Screening
Throughput
Chromatography
Ion exchange
Lead compounds
Proteins
Resins
Salts
Molecules

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Multidimensional high-throughput screening of displacers. / Rege, Kaushal; Ladiwala, Asif; Cramer, Steven M.

In: Analytical Chemistry, Vol. 77, No. 21, 01.11.2005, p. 6818-6827.

Research output: Contribution to journalArticle

Rege, Kaushal ; Ladiwala, Asif ; Cramer, Steven M. / Multidimensional high-throughput screening of displacers. In: Analytical Chemistry. 2005 ; Vol. 77, No. 21. pp. 6818-6827.
@article{e0565019873149c682f310d7396cc84e,
title = "Multidimensional high-throughput screening of displacers",
abstract = "A multidimensional, batch high-throughput screening (MD-HTS) protocol was developed to investigate the effects of various parameters on the selectivity of ion-exchange protein displacement systems. A variety of molecules were screened, and the results were employed to provided insights into the influence of displacer chemistry and concentration, resin chemistry, and mobile-phase salt counterion on the efficacy and selectivity of these nonlinear chromatographic systems. These results open up the possibility of tailoring the selectivity of displacement separations by choosing appropriate combinations of operating conditions using the MD-HTS technique. The screens were also employed for the identification of displacers and conditions for the separation of a challenging protein mixture by selective displacement chromatography. Column displacements were carried out with potential lead compounds identified from the MD-HTS screens, and the results confirmed that selective displacement could indeed be achieved for this model mixture. Furthermore, the results indicated that this approach is particularly useful when the order of elution is not changed, but the inherent selectivity is increased in the presence of the displacer. The results presented in this paper demonstrate the utility of the MD-HTS technique for rapid method development in protein ion-exchange displacement chromatography.",
author = "Kaushal Rege and Asif Ladiwala and Cramer, {Steven M.}",
year = "2005",
month = "11",
day = "1",
doi = "10.1021/ac050314a",
language = "English (US)",
volume = "77",
pages = "6818--6827",
journal = "Analytical Chemistry",
issn = "0003-2700",
publisher = "American Chemical Society",
number = "21",

}

TY - JOUR

T1 - Multidimensional high-throughput screening of displacers

AU - Rege, Kaushal

AU - Ladiwala, Asif

AU - Cramer, Steven M.

PY - 2005/11/1

Y1 - 2005/11/1

N2 - A multidimensional, batch high-throughput screening (MD-HTS) protocol was developed to investigate the effects of various parameters on the selectivity of ion-exchange protein displacement systems. A variety of molecules were screened, and the results were employed to provided insights into the influence of displacer chemistry and concentration, resin chemistry, and mobile-phase salt counterion on the efficacy and selectivity of these nonlinear chromatographic systems. These results open up the possibility of tailoring the selectivity of displacement separations by choosing appropriate combinations of operating conditions using the MD-HTS technique. The screens were also employed for the identification of displacers and conditions for the separation of a challenging protein mixture by selective displacement chromatography. Column displacements were carried out with potential lead compounds identified from the MD-HTS screens, and the results confirmed that selective displacement could indeed be achieved for this model mixture. Furthermore, the results indicated that this approach is particularly useful when the order of elution is not changed, but the inherent selectivity is increased in the presence of the displacer. The results presented in this paper demonstrate the utility of the MD-HTS technique for rapid method development in protein ion-exchange displacement chromatography.

AB - A multidimensional, batch high-throughput screening (MD-HTS) protocol was developed to investigate the effects of various parameters on the selectivity of ion-exchange protein displacement systems. A variety of molecules were screened, and the results were employed to provided insights into the influence of displacer chemistry and concentration, resin chemistry, and mobile-phase salt counterion on the efficacy and selectivity of these nonlinear chromatographic systems. These results open up the possibility of tailoring the selectivity of displacement separations by choosing appropriate combinations of operating conditions using the MD-HTS technique. The screens were also employed for the identification of displacers and conditions for the separation of a challenging protein mixture by selective displacement chromatography. Column displacements were carried out with potential lead compounds identified from the MD-HTS screens, and the results confirmed that selective displacement could indeed be achieved for this model mixture. Furthermore, the results indicated that this approach is particularly useful when the order of elution is not changed, but the inherent selectivity is increased in the presence of the displacer. The results presented in this paper demonstrate the utility of the MD-HTS technique for rapid method development in protein ion-exchange displacement chromatography.

UR - http://www.scopus.com/inward/record.url?scp=27544472256&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=27544472256&partnerID=8YFLogxK

U2 - 10.1021/ac050314a

DO - 10.1021/ac050314a

M3 - Article

C2 - 16255578

AN - SCOPUS:27544472256

VL - 77

SP - 6818

EP - 6827

JO - Analytical Chemistry

JF - Analytical Chemistry

SN - 0003-2700

IS - 21

ER -