Multi-scale temporal and spatial variation in genotypic composition of Cladophora-borne Escherichia coli populations in Lake Michigan

Brian D. Badgley, John Ferguson, Amy Vanden Heuvel, Gregory T. Kleinheinz, Colleen M. McDermott, Todd Sandrin, Julie Kinzelman, Emily A. Junion, Muruleedhara N. Byappanahalli, Richard L. Whitman, Michael J. Sadowsky

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

High concentrations of Escherichia coli in mats of Cladophora in the Great Lakes have raised concern over the continued use of this bacterium as an indicator of microbial water quality. Determining the impacts of these environmentally abundant E. coli, however, necessitates a better understanding of their ecology. In this study, the population structure of 4285 Cladophora-borne E. coli isolates, obtained over multiple three day periods from Lake Michigan Cladophora mats in 2007-2009, was examined by using DNA fingerprint analyses. In contrast to previous studies that have been done using isolates from attached Cladophora obtained over large time scales and distances, the extensive sampling done here on free-floating mats over successive days at multiple sites provided a large dataset that allowed for a detailed examination of changes in population structure over a wide range of spatial and temporal scales. While Cladophora-borne E. coli populations were highly diverse and consisted of many unique isolates, multiple clonal groups were also present and accounted for approximately 33% of all isolates examined. Patterns in population structure were also evident. At the broadest scales, E. coli populations showed some temporal clustering when examined by year, but did not show good spatial distinction among sites. E. coli population structure also showed significant patterns at much finer temporal scales. Populations were distinct on an individual mat basis at a given site, and on individual days within a single mat. Results of these studies indicate that Cladophora-borne E. coli populations consist of a mixture of stable, and possibly naturalized, strains that persist during the life of the mat, and more unique, transient strains that can change over rapid time scales. It is clear that further study of microbial processes at fine spatial and temporal scales is needed, and that caution must be taken when interpolating short term microbial dynamics from results obtained from weekly or monthly samples.

Original languageEnglish (US)
Pages (from-to)721-731
Number of pages11
JournalWater Research
Volume45
Issue number2
DOIs
StatePublished - Jan 2011
Externally publishedYes

Fingerprint

Escherichia coli
population structure
Lakes
temporal variation
spatial variation
lake
Chemical analysis
timescale
ecology
water quality
DNA
bacterium
Ecology
sampling
Water quality
Bacteria
Sampling

Keywords

  • Cladophora
  • E. coli
  • Great Lakes
  • Indicator bacteria
  • Population structure
  • Variability

ASJC Scopus subject areas

  • Water Science and Technology
  • Waste Management and Disposal
  • Pollution
  • Ecological Modeling

Cite this

Multi-scale temporal and spatial variation in genotypic composition of Cladophora-borne Escherichia coli populations in Lake Michigan. / Badgley, Brian D.; Ferguson, John; Heuvel, Amy Vanden; Kleinheinz, Gregory T.; McDermott, Colleen M.; Sandrin, Todd; Kinzelman, Julie; Junion, Emily A.; Byappanahalli, Muruleedhara N.; Whitman, Richard L.; Sadowsky, Michael J.

In: Water Research, Vol. 45, No. 2, 01.2011, p. 721-731.

Research output: Contribution to journalArticle

Badgley, BD, Ferguson, J, Heuvel, AV, Kleinheinz, GT, McDermott, CM, Sandrin, T, Kinzelman, J, Junion, EA, Byappanahalli, MN, Whitman, RL & Sadowsky, MJ 2011, 'Multi-scale temporal and spatial variation in genotypic composition of Cladophora-borne Escherichia coli populations in Lake Michigan', Water Research, vol. 45, no. 2, pp. 721-731. https://doi.org/10.1016/j.watres.2010.08.041
Badgley, Brian D. ; Ferguson, John ; Heuvel, Amy Vanden ; Kleinheinz, Gregory T. ; McDermott, Colleen M. ; Sandrin, Todd ; Kinzelman, Julie ; Junion, Emily A. ; Byappanahalli, Muruleedhara N. ; Whitman, Richard L. ; Sadowsky, Michael J. / Multi-scale temporal and spatial variation in genotypic composition of Cladophora-borne Escherichia coli populations in Lake Michigan. In: Water Research. 2011 ; Vol. 45, No. 2. pp. 721-731.
@article{1253272bcd9b4d49b19a00ca0304b982,
title = "Multi-scale temporal and spatial variation in genotypic composition of Cladophora-borne Escherichia coli populations in Lake Michigan",
abstract = "High concentrations of Escherichia coli in mats of Cladophora in the Great Lakes have raised concern over the continued use of this bacterium as an indicator of microbial water quality. Determining the impacts of these environmentally abundant E. coli, however, necessitates a better understanding of their ecology. In this study, the population structure of 4285 Cladophora-borne E. coli isolates, obtained over multiple three day periods from Lake Michigan Cladophora mats in 2007-2009, was examined by using DNA fingerprint analyses. In contrast to previous studies that have been done using isolates from attached Cladophora obtained over large time scales and distances, the extensive sampling done here on free-floating mats over successive days at multiple sites provided a large dataset that allowed for a detailed examination of changes in population structure over a wide range of spatial and temporal scales. While Cladophora-borne E. coli populations were highly diverse and consisted of many unique isolates, multiple clonal groups were also present and accounted for approximately 33{\%} of all isolates examined. Patterns in population structure were also evident. At the broadest scales, E. coli populations showed some temporal clustering when examined by year, but did not show good spatial distinction among sites. E. coli population structure also showed significant patterns at much finer temporal scales. Populations were distinct on an individual mat basis at a given site, and on individual days within a single mat. Results of these studies indicate that Cladophora-borne E. coli populations consist of a mixture of stable, and possibly naturalized, strains that persist during the life of the mat, and more unique, transient strains that can change over rapid time scales. It is clear that further study of microbial processes at fine spatial and temporal scales is needed, and that caution must be taken when interpolating short term microbial dynamics from results obtained from weekly or monthly samples.",
keywords = "Cladophora, E. coli, Great Lakes, Indicator bacteria, Population structure, Variability",
author = "Badgley, {Brian D.} and John Ferguson and Heuvel, {Amy Vanden} and Kleinheinz, {Gregory T.} and McDermott, {Colleen M.} and Todd Sandrin and Julie Kinzelman and Junion, {Emily A.} and Byappanahalli, {Muruleedhara N.} and Whitman, {Richard L.} and Sadowsky, {Michael J.}",
year = "2011",
month = "1",
doi = "10.1016/j.watres.2010.08.041",
language = "English (US)",
volume = "45",
pages = "721--731",
journal = "Water Research",
issn = "0043-1354",
publisher = "Elsevier Limited",
number = "2",

}

TY - JOUR

T1 - Multi-scale temporal and spatial variation in genotypic composition of Cladophora-borne Escherichia coli populations in Lake Michigan

AU - Badgley, Brian D.

AU - Ferguson, John

AU - Heuvel, Amy Vanden

AU - Kleinheinz, Gregory T.

AU - McDermott, Colleen M.

AU - Sandrin, Todd

AU - Kinzelman, Julie

AU - Junion, Emily A.

AU - Byappanahalli, Muruleedhara N.

AU - Whitman, Richard L.

AU - Sadowsky, Michael J.

PY - 2011/1

Y1 - 2011/1

N2 - High concentrations of Escherichia coli in mats of Cladophora in the Great Lakes have raised concern over the continued use of this bacterium as an indicator of microbial water quality. Determining the impacts of these environmentally abundant E. coli, however, necessitates a better understanding of their ecology. In this study, the population structure of 4285 Cladophora-borne E. coli isolates, obtained over multiple three day periods from Lake Michigan Cladophora mats in 2007-2009, was examined by using DNA fingerprint analyses. In contrast to previous studies that have been done using isolates from attached Cladophora obtained over large time scales and distances, the extensive sampling done here on free-floating mats over successive days at multiple sites provided a large dataset that allowed for a detailed examination of changes in population structure over a wide range of spatial and temporal scales. While Cladophora-borne E. coli populations were highly diverse and consisted of many unique isolates, multiple clonal groups were also present and accounted for approximately 33% of all isolates examined. Patterns in population structure were also evident. At the broadest scales, E. coli populations showed some temporal clustering when examined by year, but did not show good spatial distinction among sites. E. coli population structure also showed significant patterns at much finer temporal scales. Populations were distinct on an individual mat basis at a given site, and on individual days within a single mat. Results of these studies indicate that Cladophora-borne E. coli populations consist of a mixture of stable, and possibly naturalized, strains that persist during the life of the mat, and more unique, transient strains that can change over rapid time scales. It is clear that further study of microbial processes at fine spatial and temporal scales is needed, and that caution must be taken when interpolating short term microbial dynamics from results obtained from weekly or monthly samples.

AB - High concentrations of Escherichia coli in mats of Cladophora in the Great Lakes have raised concern over the continued use of this bacterium as an indicator of microbial water quality. Determining the impacts of these environmentally abundant E. coli, however, necessitates a better understanding of their ecology. In this study, the population structure of 4285 Cladophora-borne E. coli isolates, obtained over multiple three day periods from Lake Michigan Cladophora mats in 2007-2009, was examined by using DNA fingerprint analyses. In contrast to previous studies that have been done using isolates from attached Cladophora obtained over large time scales and distances, the extensive sampling done here on free-floating mats over successive days at multiple sites provided a large dataset that allowed for a detailed examination of changes in population structure over a wide range of spatial and temporal scales. While Cladophora-borne E. coli populations were highly diverse and consisted of many unique isolates, multiple clonal groups were also present and accounted for approximately 33% of all isolates examined. Patterns in population structure were also evident. At the broadest scales, E. coli populations showed some temporal clustering when examined by year, but did not show good spatial distinction among sites. E. coli population structure also showed significant patterns at much finer temporal scales. Populations were distinct on an individual mat basis at a given site, and on individual days within a single mat. Results of these studies indicate that Cladophora-borne E. coli populations consist of a mixture of stable, and possibly naturalized, strains that persist during the life of the mat, and more unique, transient strains that can change over rapid time scales. It is clear that further study of microbial processes at fine spatial and temporal scales is needed, and that caution must be taken when interpolating short term microbial dynamics from results obtained from weekly or monthly samples.

KW - Cladophora

KW - E. coli

KW - Great Lakes

KW - Indicator bacteria

KW - Population structure

KW - Variability

UR - http://www.scopus.com/inward/record.url?scp=78650417259&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=78650417259&partnerID=8YFLogxK

U2 - 10.1016/j.watres.2010.08.041

DO - 10.1016/j.watres.2010.08.041

M3 - Article

VL - 45

SP - 721

EP - 731

JO - Water Research

JF - Water Research

SN - 0043-1354

IS - 2

ER -