Abstract
Recent evidence suggests that integrin-mediated adhesion of neurons has immediate functional implications for learning and memory. In addition, adhesion of neurons to artificial substrates often determines the effectiveness and life of implants in the brain and peripheral nervous system. In this study, we present a novel biochip capable of simultaneous, quantitative, real-time monitoring of integrin-mediated adhesion and electrophysiology of primary neurons in vitro. The proposed technology combines acoustic micro-resonators capable of tracking changes in mechanics of the adhering neuronal layer, and microelectrode arrays for recording extracellular unit activity. Our results showed in four different experimental paradigms that the acoustic sensor response to adhering cells is correlated to integrin-mediated adhesion and that the micro-sensor is capable of monitoring the dynamics of neuronal adhesion over a period of 9 days. Finally, using our unique dual measurement platform, we performed simultaneous, real-time measurement of integrin-mediated adhesion and single cell electrophysiology in a neuronal culture. The sensitivities of the micro-resonators were 4-5 orders of magnitude greater than the sensitivity of the macro-scale resonators in response to adhering neurons. This multi-functional sensor platform offers insight into the interplay between integrin-mediated adhesion and neural function on a temporal resolution beyond any currently available experimental method and can therefore potentially lead to novel discoveries on the interactions between neuronal adhesion and function. This journal is
| Original language | English (US) |
|---|---|
| Pages (from-to) | 2930-2941 |
| Number of pages | 12 |
| Journal | Lab on a Chip - Miniaturisation for Chemistry and Biology |
| Volume | 12 |
| Issue number | 16 |
| DOIs | |
| State | Published - Aug 21 2012 |
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ASJC Scopus subject areas
- Biochemistry
- Chemistry(all)
- Bioengineering
- Biomedical Engineering
Cite this
Multi-modal biochip for simultaneous, real-time measurement of adhesion and electrical activity of neurons in culture. / Khraiche, Massoud; Muthuswamy, Jitendran.
In: Lab on a Chip - Miniaturisation for Chemistry and Biology, Vol. 12, No. 16, 21.08.2012, p. 2930-2941.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Multi-modal biochip for simultaneous, real-time measurement of adhesion and electrical activity of neurons in culture
AU - Khraiche, Massoud
AU - Muthuswamy, Jitendran
PY - 2012/8/21
Y1 - 2012/8/21
N2 - Recent evidence suggests that integrin-mediated adhesion of neurons has immediate functional implications for learning and memory. In addition, adhesion of neurons to artificial substrates often determines the effectiveness and life of implants in the brain and peripheral nervous system. In this study, we present a novel biochip capable of simultaneous, quantitative, real-time monitoring of integrin-mediated adhesion and electrophysiology of primary neurons in vitro. The proposed technology combines acoustic micro-resonators capable of tracking changes in mechanics of the adhering neuronal layer, and microelectrode arrays for recording extracellular unit activity. Our results showed in four different experimental paradigms that the acoustic sensor response to adhering cells is correlated to integrin-mediated adhesion and that the micro-sensor is capable of monitoring the dynamics of neuronal adhesion over a period of 9 days. Finally, using our unique dual measurement platform, we performed simultaneous, real-time measurement of integrin-mediated adhesion and single cell electrophysiology in a neuronal culture. The sensitivities of the micro-resonators were 4-5 orders of magnitude greater than the sensitivity of the macro-scale resonators in response to adhering neurons. This multi-functional sensor platform offers insight into the interplay between integrin-mediated adhesion and neural function on a temporal resolution beyond any currently available experimental method and can therefore potentially lead to novel discoveries on the interactions between neuronal adhesion and function. This journal is
AB - Recent evidence suggests that integrin-mediated adhesion of neurons has immediate functional implications for learning and memory. In addition, adhesion of neurons to artificial substrates often determines the effectiveness and life of implants in the brain and peripheral nervous system. In this study, we present a novel biochip capable of simultaneous, quantitative, real-time monitoring of integrin-mediated adhesion and electrophysiology of primary neurons in vitro. The proposed technology combines acoustic micro-resonators capable of tracking changes in mechanics of the adhering neuronal layer, and microelectrode arrays for recording extracellular unit activity. Our results showed in four different experimental paradigms that the acoustic sensor response to adhering cells is correlated to integrin-mediated adhesion and that the micro-sensor is capable of monitoring the dynamics of neuronal adhesion over a period of 9 days. Finally, using our unique dual measurement platform, we performed simultaneous, real-time measurement of integrin-mediated adhesion and single cell electrophysiology in a neuronal culture. The sensitivities of the micro-resonators were 4-5 orders of magnitude greater than the sensitivity of the macro-scale resonators in response to adhering neurons. This multi-functional sensor platform offers insight into the interplay between integrin-mediated adhesion and neural function on a temporal resolution beyond any currently available experimental method and can therefore potentially lead to novel discoveries on the interactions between neuronal adhesion and function. This journal is
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U2 - 10.1039/c2lc40190h
DO - 10.1039/c2lc40190h
M3 - Article
C2 - 22722746
AN - SCOPUS:84864237252
VL - 12
SP - 2930
EP - 2941
JO - Lab on a Chip - Miniaturisation for Chemistry and Biology
JF - Lab on a Chip - Miniaturisation for Chemistry and Biology
SN - 1473-0197
IS - 16
ER -