Mucosal Vaccines from Plant Biotechnology

Hugh Mason, Eva Thuenemann, Hiroshi Kiyono, Sarah Kessans, Nobuyuki Matoba, Tsafrir Leket-Mor

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The use of plants for production of recombinant proteins has evolved over the past 25. years. The first plant-based vaccines were expressed in stably transgenic plants, with the idea to conveniently deliver "edible vaccines" by ingestion of the antigen-containing plant material. These systems provided a proof of concept that oral delivery of vaccines in crude plant material could stimulate antigen-specific serum and mucosal antibodies. Transgenic grains like rice in particular provide a stable and robust vehicle for antigen delivery. However, some issues exist with stably transgenic plants, including relatively low expression levels and regulatory issues. Thus, many recent studies use transient expression with plant viral vectors to achieve rapid high expression in Nicotiana benthamiana, followed by purification of antigen and intranasal delivery for effective stimulation of mucosal immune responses.

Original languageEnglish (US)
Title of host publicationMucosal Immunology: Fourth Edition
PublisherElsevier Inc.
Pages1271-1289
Number of pages19
Volume2-2
ISBN (Print)9780124159754, 9780124158474
DOIs
StatePublished - Apr 1 2015

Keywords

  • Animal vaccine
  • Cholera toxin
  • Hepatitis B
  • HIV
  • MucoRice
  • Nicotiana benthamiana
  • Norovirus
  • Plant vaccine

ASJC Scopus subject areas

  • Medicine(all)
  • Immunology and Microbiology(all)

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  • Cite this

    Mason, H., Thuenemann, E., Kiyono, H., Kessans, S., Matoba, N., & Leket-Mor, T. (2015). Mucosal Vaccines from Plant Biotechnology. In Mucosal Immunology: Fourth Edition (Vol. 2-2, pp. 1271-1289). Elsevier Inc.. https://doi.org/10.1016/B978-0-12-415847-4.00065-3