Messbauérovskaia spektroskopiia kletok tsianobakterii Synechocystis sp. PCC 6803 bez fotosistemy I i s neaktivnymi fikobilisomami.

Translated title of the contribution: Mossbauer spectroscopy of cells of cyanobacteria Synechocystis sp. PCC 6803 devoid of photosystem I and containing inactive phycobilisomes

A. A. Novakova, L. N. Davletshina, I. V. Elanskaia, A. I. Aleksandrov, T. I. Kiseleva, B. K. Semin, I. I. Ivanov, Willem Vermaas, A. B. Rubin

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Mossbauer spectra of the psaAB mutant of Synechocystis sp. PPC 6803 devoid of photosystem I grown in a 57Fe-containing medium were measured. The spectrum is a broadened doublet whose size (about 20%) and parameters (isomeric shift delta = 0.3 mm/s and quadrupole splitting delta = 0.8 mm/s) suggest the presence of abundant nanoclusters of Fe3+ oxides in a superparamagnetic state tightly bound to the membrane. Treatment of cells with EDTA was accompanied by a substantial (tenfold) decrease in the amount of iron nonspecifically bound to the membrane and the appearance of Fe2+ localized, probably, inside cells and/or cell membranes. In addition, the spectrum of washed cells exhibited superfine magnetic splitting due to iron oxide clusters greater in size than nanoclusters present in the membrane prior to EDTA treatment.

Original languageUndefined/Unknown
Pages (from-to)482-485
Number of pages4
JournalBiofizika
Volume46
Issue number3
StatePublished - May 2001
Externally publishedYes

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Phycobilisomes
Mossbauer Spectroscopy
Synechocystis
Photosystem I Protein Complex
Cyanobacteria
Edetic Acid
Membranes
Oxides
Iron
Cell Membrane

ASJC Scopus subject areas

  • Biophysics

Cite this

Novakova, A. A., Davletshina, L. N., Elanskaia, I. V., Aleksandrov, A. I., Kiseleva, T. I., Semin, B. K., ... Rubin, A. B. (2001). Messbauérovskaia spektroskopiia kletok tsianobakterii Synechocystis sp. PCC 6803 bez fotosistemy I i s neaktivnymi fikobilisomami. Biofizika, 46(3), 482-485.

Messbauérovskaia spektroskopiia kletok tsianobakterii Synechocystis sp. PCC 6803 bez fotosistemy I i s neaktivnymi fikobilisomami. / Novakova, A. A.; Davletshina, L. N.; Elanskaia, I. V.; Aleksandrov, A. I.; Kiseleva, T. I.; Semin, B. K.; Ivanov, I. I.; Vermaas, Willem; Rubin, A. B.

In: Biofizika, Vol. 46, No. 3, 05.2001, p. 482-485.

Research output: Contribution to journalArticle

Novakova, AA, Davletshina, LN, Elanskaia, IV, Aleksandrov, AI, Kiseleva, TI, Semin, BK, Ivanov, II, Vermaas, W & Rubin, AB 2001, 'Messbauérovskaia spektroskopiia kletok tsianobakterii Synechocystis sp. PCC 6803 bez fotosistemy I i s neaktivnymi fikobilisomami.', Biofizika, vol. 46, no. 3, pp. 482-485.
Novakova AA, Davletshina LN, Elanskaia IV, Aleksandrov AI, Kiseleva TI, Semin BK et al. Messbauérovskaia spektroskopiia kletok tsianobakterii Synechocystis sp. PCC 6803 bez fotosistemy I i s neaktivnymi fikobilisomami. Biofizika. 2001 May;46(3):482-485.
Novakova, A. A. ; Davletshina, L. N. ; Elanskaia, I. V. ; Aleksandrov, A. I. ; Kiseleva, T. I. ; Semin, B. K. ; Ivanov, I. I. ; Vermaas, Willem ; Rubin, A. B. / Messbauérovskaia spektroskopiia kletok tsianobakterii Synechocystis sp. PCC 6803 bez fotosistemy I i s neaktivnymi fikobilisomami. In: Biofizika. 2001 ; Vol. 46, No. 3. pp. 482-485.
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abstract = "Mossbauer spectra of the psaAB mutant of Synechocystis sp. PPC 6803 devoid of photosystem I grown in a 57Fe-containing medium were measured. The spectrum is a broadened doublet whose size (about 20{\%}) and parameters (isomeric shift delta = 0.3 mm/s and quadrupole splitting delta = 0.8 mm/s) suggest the presence of abundant nanoclusters of Fe3+ oxides in a superparamagnetic state tightly bound to the membrane. Treatment of cells with EDTA was accompanied by a substantial (tenfold) decrease in the amount of iron nonspecifically bound to the membrane and the appearance of Fe2+ localized, probably, inside cells and/or cell membranes. In addition, the spectrum of washed cells exhibited superfine magnetic splitting due to iron oxide clusters greater in size than nanoclusters present in the membrane prior to EDTA treatment.",
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