Monoclonal antibodies to the light-harvesting chlorophyll a/b protein complex of photosystem II.

S. C. Darr, S. C. Somerville, C. J. Arntzen

Research output: Contribution to journalArticle

72 Scopus citations

Abstract

A collection of 17 monoclonal antibodies elicited against the light-harvesting chlorophyll a/b protein complex which serves photosystem II (LHC-II) of Pisum sativum shows six classes of binding specificity. Antibodies of two of the classes recognize a single polypeptide (the 28- or the 26- kD polypeptides), thereby suggesting that the two proteins are not derived from a common precursor. Other classes of antibodies cross-react with several polypeptides of LHC-II or with polypeptides of both LHC-II and the light-harvesting chlorophyll a/b polypeptides of photosystem I (LHC-I), indicating that there are structural similarities among the polypeptides of LHC-II and LHC-I. The evidence for protein processing by which the 26-, 25.5-, and 24.5-kD polypeptides are derived from a common precursor polypeptide is discussed. Binding studies using antibodies specific for individual LHC-II polypeptides were used to quantify the number of antigenic polypeptides in the thylakoid membrane. 27 copies of the 26-kD polypeptide and two copies of the 28-kD polypeptide were found per 400 chlorophylls. In the chlorina f2 mutant of barley, and in intermittent light-treated barley seedlings, the amount of the 26-kD polypeptide in the thylakoid membranes was greatly reduced, while the amount of 28-kD polypeptide was apparently not affected. We propose that stable insertion and assembly of the 28-kD polypeptide, unlike the 26-kD polypeptide, is not regulated by the presence of chlorophyll b.

Original languageEnglish (US)
Pages (from-to)733-740
Number of pages8
JournalThe Journal of cell biology
Volume103
Issue number3
DOIs
StatePublished - Sep 1986

ASJC Scopus subject areas

  • Cell Biology

Fingerprint Dive into the research topics of 'Monoclonal antibodies to the light-harvesting chlorophyll a/b protein complex of photosystem II.'. Together they form a unique fingerprint.

  • Cite this