Monitoring hippocampus electrical activity in vitro on an elastically deformable microelectrode array

Zhe Yu, Oliver Graudejus, Candice Tsay, Stéphanie P. Lacour, Sigurd Wagner, Barclay Morrison

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

Interfacing electronics and recording electrophysiological activity in mechanically active biological tissues is challenging. This challenge extends to recording neural function of brain tissue in the setting of traumatic brain injury (TBI), which is caused by rapid (within hundreds of milliseconds) and large (greater than 5% strain) brain deformation. Interfacing electrodes must be biocompatible on multiple levels and should deform with the tissue to prevent additional mechanical damage. We describe an elastically stretchable microelectrode array (SMEA) that is capable of undergoing large, biaxial, 2-D stretch while remaining functional. The new SMEA consists of elastically stretchable thin metal films on a silicone membrane. It can stimulate and detect electrical activity from cultured brain tissue (hippocampal slices), before, during, and after large biaxial deformation. We have incorporated the SMEA into a well-characterized in vitro TBI research platform, which reproduces the biomechanics of TBI by stretching the SMEA and the adherent brain slice culture. Mechanical injury parameters, such as strain and strain rate, can be precisely controlled to generate specific levels of damage. The SMEA allowed for quantification of neuronal function both before and after injury, without breaking culture sterility or repositioning the electrodes for the injury event, thus enabling serial and long-term measurements. We report tests of the SMEA and an initial application to study the effect of mechanical stimuli on neuron function, which could be employed as a high-content, drug-screening platform for TBI.

Original languageEnglish (US)
Pages (from-to)1135-1145
Number of pages11
JournalJournal of Neurotrauma
Volume26
Issue number7
DOIs
StatePublished - Jul 1 2009
Externally publishedYes

Fingerprint

Microelectrodes
Hippocampus
Brain
Wounds and Injuries
Electrodes
Preclinical Drug Evaluations
Silicones
Biomechanical Phenomena
Infertility
In Vitro Techniques
Metals
Neurons
Membranes
Traumatic Brain Injury
Research

Keywords

  • Electrophysiology
  • In vitro studies
  • Outcome measures
  • Traumatic brain injury

ASJC Scopus subject areas

  • Clinical Neurology

Cite this

Monitoring hippocampus electrical activity in vitro on an elastically deformable microelectrode array. / Yu, Zhe; Graudejus, Oliver; Tsay, Candice; Lacour, Stéphanie P.; Wagner, Sigurd; Morrison, Barclay.

In: Journal of Neurotrauma, Vol. 26, No. 7, 01.07.2009, p. 1135-1145.

Research output: Contribution to journalArticle

Yu, Zhe ; Graudejus, Oliver ; Tsay, Candice ; Lacour, Stéphanie P. ; Wagner, Sigurd ; Morrison, Barclay. / Monitoring hippocampus electrical activity in vitro on an elastically deformable microelectrode array. In: Journal of Neurotrauma. 2009 ; Vol. 26, No. 7. pp. 1135-1145.
@article{5ea10cb32be04da58815a3cbfda8ca5b,
title = "Monitoring hippocampus electrical activity in vitro on an elastically deformable microelectrode array",
abstract = "Interfacing electronics and recording electrophysiological activity in mechanically active biological tissues is challenging. This challenge extends to recording neural function of brain tissue in the setting of traumatic brain injury (TBI), which is caused by rapid (within hundreds of milliseconds) and large (greater than 5{\%} strain) brain deformation. Interfacing electrodes must be biocompatible on multiple levels and should deform with the tissue to prevent additional mechanical damage. We describe an elastically stretchable microelectrode array (SMEA) that is capable of undergoing large, biaxial, 2-D stretch while remaining functional. The new SMEA consists of elastically stretchable thin metal films on a silicone membrane. It can stimulate and detect electrical activity from cultured brain tissue (hippocampal slices), before, during, and after large biaxial deformation. We have incorporated the SMEA into a well-characterized in vitro TBI research platform, which reproduces the biomechanics of TBI by stretching the SMEA and the adherent brain slice culture. Mechanical injury parameters, such as strain and strain rate, can be precisely controlled to generate specific levels of damage. The SMEA allowed for quantification of neuronal function both before and after injury, without breaking culture sterility or repositioning the electrodes for the injury event, thus enabling serial and long-term measurements. We report tests of the SMEA and an initial application to study the effect of mechanical stimuli on neuron function, which could be employed as a high-content, drug-screening platform for TBI.",
keywords = "Electrophysiology, In vitro studies, Outcome measures, Traumatic brain injury",
author = "Zhe Yu and Oliver Graudejus and Candice Tsay and Lacour, {St{\'e}phanie P.} and Sigurd Wagner and Barclay Morrison",
year = "2009",
month = "7",
day = "1",
doi = "10.1089/neu.2008.0810",
language = "English (US)",
volume = "26",
pages = "1135--1145",
journal = "Journal of Neurotrauma",
issn = "0897-7151",
publisher = "Mary Ann Liebert Inc.",
number = "7",

}

TY - JOUR

T1 - Monitoring hippocampus electrical activity in vitro on an elastically deformable microelectrode array

AU - Yu, Zhe

AU - Graudejus, Oliver

AU - Tsay, Candice

AU - Lacour, Stéphanie P.

AU - Wagner, Sigurd

AU - Morrison, Barclay

PY - 2009/7/1

Y1 - 2009/7/1

N2 - Interfacing electronics and recording electrophysiological activity in mechanically active biological tissues is challenging. This challenge extends to recording neural function of brain tissue in the setting of traumatic brain injury (TBI), which is caused by rapid (within hundreds of milliseconds) and large (greater than 5% strain) brain deformation. Interfacing electrodes must be biocompatible on multiple levels and should deform with the tissue to prevent additional mechanical damage. We describe an elastically stretchable microelectrode array (SMEA) that is capable of undergoing large, biaxial, 2-D stretch while remaining functional. The new SMEA consists of elastically stretchable thin metal films on a silicone membrane. It can stimulate and detect electrical activity from cultured brain tissue (hippocampal slices), before, during, and after large biaxial deformation. We have incorporated the SMEA into a well-characterized in vitro TBI research platform, which reproduces the biomechanics of TBI by stretching the SMEA and the adherent brain slice culture. Mechanical injury parameters, such as strain and strain rate, can be precisely controlled to generate specific levels of damage. The SMEA allowed for quantification of neuronal function both before and after injury, without breaking culture sterility or repositioning the electrodes for the injury event, thus enabling serial and long-term measurements. We report tests of the SMEA and an initial application to study the effect of mechanical stimuli on neuron function, which could be employed as a high-content, drug-screening platform for TBI.

AB - Interfacing electronics and recording electrophysiological activity in mechanically active biological tissues is challenging. This challenge extends to recording neural function of brain tissue in the setting of traumatic brain injury (TBI), which is caused by rapid (within hundreds of milliseconds) and large (greater than 5% strain) brain deformation. Interfacing electrodes must be biocompatible on multiple levels and should deform with the tissue to prevent additional mechanical damage. We describe an elastically stretchable microelectrode array (SMEA) that is capable of undergoing large, biaxial, 2-D stretch while remaining functional. The new SMEA consists of elastically stretchable thin metal films on a silicone membrane. It can stimulate and detect electrical activity from cultured brain tissue (hippocampal slices), before, during, and after large biaxial deformation. We have incorporated the SMEA into a well-characterized in vitro TBI research platform, which reproduces the biomechanics of TBI by stretching the SMEA and the adherent brain slice culture. Mechanical injury parameters, such as strain and strain rate, can be precisely controlled to generate specific levels of damage. The SMEA allowed for quantification of neuronal function both before and after injury, without breaking culture sterility or repositioning the electrodes for the injury event, thus enabling serial and long-term measurements. We report tests of the SMEA and an initial application to study the effect of mechanical stimuli on neuron function, which could be employed as a high-content, drug-screening platform for TBI.

KW - Electrophysiology

KW - In vitro studies

KW - Outcome measures

KW - Traumatic brain injury

UR - http://www.scopus.com/inward/record.url?scp=69249127063&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=69249127063&partnerID=8YFLogxK

U2 - 10.1089/neu.2008.0810

DO - 10.1089/neu.2008.0810

M3 - Article

VL - 26

SP - 1135

EP - 1145

JO - Journal of Neurotrauma

JF - Journal of Neurotrauma

SN - 0897-7151

IS - 7

ER -