TY - JOUR
T1 - Molecular scale origin of surface plasmon resonance biosensors
AU - Yu, Hui
AU - Shan, Xiaonan
AU - Wang, Shaopeng
AU - Chen, Hongyuan
AU - Tao, Nongjian
N1 - Publisher Copyright:
© 2014 American Chemical Society.
PY - 2014/9/1
Y1 - 2014/9/1
N2 - Surface plasmon resonance (SPR) has become an indispensable tool for label-free detection and quantification of molecular binding. Traditionally, the principle of SPR biosensors is described with a stratified medium model, in which discrete molecules are approximated with a uniform thin film. With the recent technical advances, SPR can now detect extremely low coverage of molecules, which raises the question of the validity of the traditional model. Here, we present combined theoretical, numerical and experimental analysis of SPR detection principle by considering the discrete nature of the molecules (particles). Our results show that the stratified medium model can provide reasonable description of SPR biosensors for relatively high coverage and weakly scattering samples. However, interference between the SPR images of individual particles needs to be considered for high spatial resolution images and for strong scattering samples at certain incident angles of light.
AB - Surface plasmon resonance (SPR) has become an indispensable tool for label-free detection and quantification of molecular binding. Traditionally, the principle of SPR biosensors is described with a stratified medium model, in which discrete molecules are approximated with a uniform thin film. With the recent technical advances, SPR can now detect extremely low coverage of molecules, which raises the question of the validity of the traditional model. Here, we present combined theoretical, numerical and experimental analysis of SPR detection principle by considering the discrete nature of the molecules (particles). Our results show that the stratified medium model can provide reasonable description of SPR biosensors for relatively high coverage and weakly scattering samples. However, interference between the SPR images of individual particles needs to be considered for high spatial resolution images and for strong scattering samples at certain incident angles of light.
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U2 - 10.1021/ac501363z
DO - 10.1021/ac501363z
M3 - Article
AN - SCOPUS:84913582391
SN - 0003-2700
VL - 86
SP - 8992
EP - 8997
JO - Analytical Chemistry
JF - Analytical Chemistry
IS - 18
ER -