TY - JOUR
T1 - Molecular robots guided by prescriptive landscapes
AU - Lund, Kyle
AU - Manzo, Anthony J.
AU - Dabby, Nadine
AU - Michelotti, Nicole
AU - Johnson-Buck, Alexander
AU - Nangreave, Jeanette
AU - Taylor, Steven
AU - Pei, Renjun
AU - Stojanovic, Milan N.
AU - Walter, Nils G.
AU - Winfree, Erik
AU - Yan, Hao
PY - 2010/5/13
Y1 - 2010/5/13
N2 - Traditional robots1 rely for their function on computing, to store internal representations of their goals and environment and to coordinate sensing and any actuation of components required in response. Moving robotics to the single-molecule level is possible in principle, but requires facing the limited ability of individual molecules to store complex information and programs. One strategy to overcome this problem is to use systems that can obtain complex behaviour from the interaction of simple robots with their environment2-4. A first step in this direction was the development of DNA walkers, which have developed from being non-autonomous6,7 to being capable of directed but brief motion on one-dimensional tracks 8-11. Here we demonstrate that previously developed random walkersg12-so-called molecular spiders that comprise a streptavidin molecule as an inert body and three deoxyribozymes as catalytic legsg show elementary robotic behaviour when interacting with a precisely defined environment. Single-molecule microscopy observations confirm that such walkers achieve directional movement by sensing and modifying tracks of substrate molecules laid out on a two-dimensional DNA origami landscape. When using appropriately designed DNA origami, the molecular spiders autonomously carry out sequences of actions such as ĝ€̃ startĝ€™, ĝ€̃ followĝ€™, ĝ€̃ turnĝ€™ and ĝ€̃ stopĝ€™. We anticipate that this strategy will result in more complex robotic behaviour at the molecular level if additional control mechanisms are incorporated. One example might be interactions between multiple molecular robots leading to collective behaviour; another might be the ability to read and transform secondary cues on the DNA origami landscape as a means of implementing Turing-universal algorithmic behaviour.
AB - Traditional robots1 rely for their function on computing, to store internal representations of their goals and environment and to coordinate sensing and any actuation of components required in response. Moving robotics to the single-molecule level is possible in principle, but requires facing the limited ability of individual molecules to store complex information and programs. One strategy to overcome this problem is to use systems that can obtain complex behaviour from the interaction of simple robots with their environment2-4. A first step in this direction was the development of DNA walkers, which have developed from being non-autonomous6,7 to being capable of directed but brief motion on one-dimensional tracks 8-11. Here we demonstrate that previously developed random walkersg12-so-called molecular spiders that comprise a streptavidin molecule as an inert body and three deoxyribozymes as catalytic legsg show elementary robotic behaviour when interacting with a precisely defined environment. Single-molecule microscopy observations confirm that such walkers achieve directional movement by sensing and modifying tracks of substrate molecules laid out on a two-dimensional DNA origami landscape. When using appropriately designed DNA origami, the molecular spiders autonomously carry out sequences of actions such as ĝ€̃ startĝ€™, ĝ€̃ followĝ€™, ĝ€̃ turnĝ€™ and ĝ€̃ stopĝ€™. We anticipate that this strategy will result in more complex robotic behaviour at the molecular level if additional control mechanisms are incorporated. One example might be interactions between multiple molecular robots leading to collective behaviour; another might be the ability to read and transform secondary cues on the DNA origami landscape as a means of implementing Turing-universal algorithmic behaviour.
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U2 - 10.1038/nature09012
DO - 10.1038/nature09012
M3 - Article
C2 - 20463735
AN - SCOPUS:77952392836
SN - 0028-0836
VL - 465
SP - 206
EP - 209
JO - Nature
JF - Nature
IS - 7295
ER -