Molecular recognition using corona phase complexes made of synthetic polymers adsorbed on carbon nanotubes

Jingqing Zhang, Markita P. Landry, Paul W. Barone, Jong Ho Kim, Shangchao Lin, Zachary W. Ulissi, Dahua Lin, Bin Mu, Ardemis A. Boghossian, Andrew J. Hilmer, Alina Rwei, Allison C. Hinckley, Sebastian Kruss, Mia A. Shandell, Nitish Nair, Steven Blake, Fatih Şen, Selda Şen, Robert G. Cray, Deyu LiKyungsuk Yum, Jin Ho Ahn, Hong Jin, Daniel A. Heller, John M. Essigmann, Daniel Blankschtein, Michael S. Strano

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Nanomaterials are often functionalized with biological ligands to enable their use as sensors of biological activity. However, the intricacies of nano-bio interactions are poorly understood, which hampers our ability to design nanomaterial-based sensors. Current experimental tools have been unable to visualize interactions occurring on the nano-bio interface with the spatial and temporal resolution needed to quantify biological interactions at their fundamental length and time scales. To fill the need for concurrent visualization of nanoparticles and biomolecules, we have combined two common microscopy techniques, one being for the study of biomolecules and the other for the study of nanoparticles, into a single instrument that has the capacity to study both nanoparticles and biological molecules simultaneously with spatial and temporal resolution that is appropriate for nanoscale interactions. This novel instrument has been used for the characterization of high-sensitivity sensors by designing synthetic biological polymers to selectively encapsulate single-wall carbon nanotubes. The design of synthetic sensing tools based on nanoparticle-biomolecule hybrids is promising for areas in need of high-specificity sensors, such as label-free detection of molecules within a cell, nanoparticle-based diagnostic tools, and nanoscale therapeutics. We introduce three examples of high-sensitivity and high-selectivity synthetic sensors that have the ability to detect a variety of molecules on a single-molecule scale: riboflavin, L-thyroxine, and oestradiol. These sensors have been used to detect and quantify riboflavin levels within a live murine macrophage cell in real-time. The findings provided herein will enable the development of early-onset diagnostic tools at the level of a single cell.

Original languageEnglish (US)
Title of host publicationProceedings - 2014 40th Annual Northeast Bioengineering Conference, NEBEC 2014
PublisherInstitute of Electrical and Electronics Engineers Inc.
ISBN (Electronic)9781479937288
DOIs
StatePublished - Dec 2 2014
Event2014 40th Annual Northeast Bioengineering Conference, NEBEC 2014 - Boston, United States
Duration: Apr 25 2014Apr 27 2014

Publication series

NameProceedings of the IEEE Annual Northeast Bioengineering Conference, NEBEC
Volume2014-December
ISSN (Print)1071-121X
ISSN (Electronic)2160-7001

Other

Other2014 40th Annual Northeast Bioengineering Conference, NEBEC 2014
CountryUnited States
CityBoston
Period4/25/144/27/14

Keywords

  • Carbon nanotubes
  • in vivo detection
  • sensors
  • single-molecule imaging
  • synthetic antibodies

ASJC Scopus subject areas

  • Bioengineering

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  • Cite this

    Zhang, J., Landry, M. P., Barone, P. W., Kim, J. H., Lin, S., Ulissi, Z. W., Lin, D., Mu, B., Boghossian, A. A., Hilmer, A. J., Rwei, A., Hinckley, A. C., Kruss, S., Shandell, M. A., Nair, N., Blake, S., Şen, F., Şen, S., Cray, R. G., ... Strano, M. S. (2014). Molecular recognition using corona phase complexes made of synthetic polymers adsorbed on carbon nanotubes. In Proceedings - 2014 40th Annual Northeast Bioengineering Conference, NEBEC 2014 [6972997] (Proceedings of the IEEE Annual Northeast Bioengineering Conference, NEBEC; Vol. 2014-December). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/NEBEC.2014.6972997