TY - JOUR
T1 - Molecular Phylogenetic Position of Microjoenia (Parabasalia: Spirotrichonymphea) from Reticulitermes and Hodotermopsis Termite Hosts
AU - Gile, Gillian H.
AU - Taerum, Stephen J.
AU - Jasso-Selles, Daniel E.
AU - Sillam-Dussès, David
AU - Ohkuma, Moriya
AU - Kitade, Osamu
AU - Noda, Satoko
N1 - Funding Information:
The authors would like to thank Bradley Bobbett and Tomoe Kurisaki for technical assistance. This work was supported by the National Science Foundation (DEB-2045329) and the School of Life Sciences and the College of Liberal Arts and Sciences (startup grant to G.H.G.). This work was also supported in part by Grants-in-Aid for Scientific Research from Japan Society for the Promotion of Science (JSPS), JP19K06334 (to S.N.), JP17H01510 (to O.K.), JP19H05689 and JP19H05679 (to M.O.).
Publisher Copyright:
© 2021 Elsevier GmbH
PY - 2021/10/1
Y1 - 2021/10/1
N2 - Microjoenia are obligate symbionts of termites. The genus was erected in 1892 for small cells with many flagella that insert near, but not directly from, the cell apex, and an axostyle that can protrude from the cell posterior. Although ultrastructural studies have been carried out on three Microjoenia species to date, no molecular data have been directly attributed to any species. Microjoenia are classified within the parabasalian class Spirotrichonymphea, which is characterized by flagellar bands that emerge near the cell apex and proceed posteriorly in a right-handed helix. In Microjoenia, however, the flagellar bands are very short and proceed longitudinally or with a weakly observable helix. In this study, we have amplified and sequenced the 18S ribosomal RNA gene from individually isolated Microjoenia cells from Reticulitermes and Hodotermopsis hosts as part of an ongoing effort to understand the phylogeny of Spirotrichonymphea and their coevolution with termites. In our 18S rRNA gene phylogeny, Microjoenia forms the sister lineage to Spirotrichonympha, though many other evolutionary relationships within Spirotrichonymphea remain unresolved.
AB - Microjoenia are obligate symbionts of termites. The genus was erected in 1892 for small cells with many flagella that insert near, but not directly from, the cell apex, and an axostyle that can protrude from the cell posterior. Although ultrastructural studies have been carried out on three Microjoenia species to date, no molecular data have been directly attributed to any species. Microjoenia are classified within the parabasalian class Spirotrichonymphea, which is characterized by flagellar bands that emerge near the cell apex and proceed posteriorly in a right-handed helix. In Microjoenia, however, the flagellar bands are very short and proceed longitudinally or with a weakly observable helix. In this study, we have amplified and sequenced the 18S ribosomal RNA gene from individually isolated Microjoenia cells from Reticulitermes and Hodotermopsis hosts as part of an ongoing effort to understand the phylogeny of Spirotrichonymphea and their coevolution with termites. In our 18S rRNA gene phylogeny, Microjoenia forms the sister lineage to Spirotrichonympha, though many other evolutionary relationships within Spirotrichonymphea remain unresolved.
KW - Coevolution
KW - Metamonada
KW - symbiosis
KW - termite
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U2 - 10.1016/j.protis.2021.125836
DO - 10.1016/j.protis.2021.125836
M3 - Article
C2 - 34757297
AN - SCOPUS:85118326401
SN - 1434-4610
VL - 172
JO - Protist
JF - Protist
IS - 5-6
M1 - 125836
ER -