Molecular mimicry between Fc receptor and S peplomer protein of mouse hepatitis virus, bovine corona virus, and transmissible gastroenteritis virus

E. L. Oleszak, J. Kuzmak, Brenda Hogue, R. Parr, E. W. Collisson, L. S. Rodkey, J. L. Leibowitz

Research output: Contribution to journalArticle

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Abstract

We have previously demonstrated molecular mimicry between the S peplomer protein of mouse hepatitis virus (MHV) and Fc gamma R (FcγR). A monoclonal antibody (MAb) to mouse FcγR (2.4G2 anti-FcγR MAb), purified rabbit immunoglobulin, but not their F(ab')2 fragments, as well as mouse and rat IgG, immunoprecipitated (1) recombinant S peplomer protein expressed by a vaccinia virus recombinant in human, rabbit, and mouse cells, and (2) natural S peplomer protein from cells infected with several strains of MHV and MHV escape mutants. We report here results of studies documenting molecular mimicry between FcγR and S peplomer protein of viruses representing three distinct antigenic subgroups of the Coronaviridae. We have shown a molecular mimicry between the S peplomer protein of bovine corona virus (BCV) and FcγR. The 2.4G2 anti-FcγR MAb, rabbit IgG, but not its F(ab')2 fragments, as well as homologous bovine serum, free of anti-BCV antibodies, immunoprecipitated S peplomer protein of BCV (Mebus strain). In contrast, we did not find molecular mimicry between S peplomer protein of human corona virus (HCV-OC43) and FcγR. Although the OC43 virus belongs to the same antigenic group as MHV and BCV, MAb specific for human FcγR I or FcγR II and purified human IgG1, IgG2, and IgG3 myeloma proteins did not immunoprecipitate the S peplomer protein from HCV-OC43-infected RD cells. In addition, we did demonstrate molecular mimicry between the S peplomer protein of porcine transmissible gastroenteritis virus (TGEV) and FcγR. TGEV belongs to the second antigenic subgroup of coronaviridae. Homologous swine IgG, but not its F(ab')2 fragments, immunoprecipitated from TGEV-infected cells a 195-kDa polypeptide corresponding to the TGEV S peplomer protein. We have also examined whether there is a molecular mimicry between S peplomer protein of infectious bronchitis virus (IBV) and FcγR. Nonimmune chicken IgG did not immunoprecipitate the S peplomer protein from IBV-infected chicken embryo fibroblasts or Vero cells, suggesting that there is no molecular mimicry between the IBV-S and FcγR. In conclusion, we have demonstrated molecular mimicry between FcγR and S peplomer protein of three members of Coronaviridae, namely MHV, BCV, and TGEV. In contrast, the S peplomer protein of two other members of Coronaviridae, namely HCV-OC43 and IBV, did not exhibit any molecular mimicry with FcγR.

Original languageEnglish (US)
Pages (from-to)1-8
Number of pages8
JournalHybridoma
Volume14
Issue number1
StatePublished - 1995
Externally publishedYes

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Transmissible gastroenteritis virus
Molecular Mimicry
Fc Receptors
Protein S
Coronaviridae
Viruses
Infectious bronchitis virus
Murine hepatitis virus
Immunoglobulin G
Monoclonal Antibodies
Viral Envelope Proteins
Rabbits
Chickens
Myeloma Proteins
MHV surface projection glycoprotein
Vero Cells
Vaccinia virus
Immunoglobulins
Swine
Embryonic Structures

ASJC Scopus subject areas

  • Genetics
  • Immunology

Cite this

Oleszak, E. L., Kuzmak, J., Hogue, B., Parr, R., Collisson, E. W., Rodkey, L. S., & Leibowitz, J. L. (1995). Molecular mimicry between Fc receptor and S peplomer protein of mouse hepatitis virus, bovine corona virus, and transmissible gastroenteritis virus. Hybridoma, 14(1), 1-8.

Molecular mimicry between Fc receptor and S peplomer protein of mouse hepatitis virus, bovine corona virus, and transmissible gastroenteritis virus. / Oleszak, E. L.; Kuzmak, J.; Hogue, Brenda; Parr, R.; Collisson, E. W.; Rodkey, L. S.; Leibowitz, J. L.

In: Hybridoma, Vol. 14, No. 1, 1995, p. 1-8.

Research output: Contribution to journalArticle

Oleszak, EL, Kuzmak, J, Hogue, B, Parr, R, Collisson, EW, Rodkey, LS & Leibowitz, JL 1995, 'Molecular mimicry between Fc receptor and S peplomer protein of mouse hepatitis virus, bovine corona virus, and transmissible gastroenteritis virus', Hybridoma, vol. 14, no. 1, pp. 1-8.
Oleszak, E. L. ; Kuzmak, J. ; Hogue, Brenda ; Parr, R. ; Collisson, E. W. ; Rodkey, L. S. ; Leibowitz, J. L. / Molecular mimicry between Fc receptor and S peplomer protein of mouse hepatitis virus, bovine corona virus, and transmissible gastroenteritis virus. In: Hybridoma. 1995 ; Vol. 14, No. 1. pp. 1-8.
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AU - Kuzmak, J.

AU - Hogue, Brenda

AU - Parr, R.

AU - Collisson, E. W.

AU - Rodkey, L. S.

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N2 - We have previously demonstrated molecular mimicry between the S peplomer protein of mouse hepatitis virus (MHV) and Fc gamma R (FcγR). A monoclonal antibody (MAb) to mouse FcγR (2.4G2 anti-FcγR MAb), purified rabbit immunoglobulin, but not their F(ab')2 fragments, as well as mouse and rat IgG, immunoprecipitated (1) recombinant S peplomer protein expressed by a vaccinia virus recombinant in human, rabbit, and mouse cells, and (2) natural S peplomer protein from cells infected with several strains of MHV and MHV escape mutants. We report here results of studies documenting molecular mimicry between FcγR and S peplomer protein of viruses representing three distinct antigenic subgroups of the Coronaviridae. We have shown a molecular mimicry between the S peplomer protein of bovine corona virus (BCV) and FcγR. The 2.4G2 anti-FcγR MAb, rabbit IgG, but not its F(ab')2 fragments, as well as homologous bovine serum, free of anti-BCV antibodies, immunoprecipitated S peplomer protein of BCV (Mebus strain). In contrast, we did not find molecular mimicry between S peplomer protein of human corona virus (HCV-OC43) and FcγR. Although the OC43 virus belongs to the same antigenic group as MHV and BCV, MAb specific for human FcγR I or FcγR II and purified human IgG1, IgG2, and IgG3 myeloma proteins did not immunoprecipitate the S peplomer protein from HCV-OC43-infected RD cells. In addition, we did demonstrate molecular mimicry between the S peplomer protein of porcine transmissible gastroenteritis virus (TGEV) and FcγR. TGEV belongs to the second antigenic subgroup of coronaviridae. Homologous swine IgG, but not its F(ab')2 fragments, immunoprecipitated from TGEV-infected cells a 195-kDa polypeptide corresponding to the TGEV S peplomer protein. We have also examined whether there is a molecular mimicry between S peplomer protein of infectious bronchitis virus (IBV) and FcγR. Nonimmune chicken IgG did not immunoprecipitate the S peplomer protein from IBV-infected chicken embryo fibroblasts or Vero cells, suggesting that there is no molecular mimicry between the IBV-S and FcγR. In conclusion, we have demonstrated molecular mimicry between FcγR and S peplomer protein of three members of Coronaviridae, namely MHV, BCV, and TGEV. In contrast, the S peplomer protein of two other members of Coronaviridae, namely HCV-OC43 and IBV, did not exhibit any molecular mimicry with FcγR.

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