Molecular mechanisms for photosynthetic carbon partitioning into storage neutral lipids in Nannochloropsis oceanica under nitrogen-depletion conditions

Jing Jia, Danxiang Han, Henri G. Gerken, Yantao Li, Milton Sommerfeld, Qiang Hu, Jian Xu

Research output: Contribution to journalArticle

86 Citations (Scopus)

Abstract

Polysaccharides are a major carbon/energy-reservoir in microalgae, yet their relationship with another form of carbon/energy storage, triacylglycerol (TAG), is poorly understood. Here employing oleaginous microalga Nannochloropsis oceanica as a model, we probed the crosstalk between carbohydrate metabolism and TAG accumulation by tracking the temporal dynamics of lipidomes, monosaccharides and polysaccharides and transcripts of selected genes over 14. days under nitrogen-depleted (N. -) and nitrogen-replete (N. +) conditions. Glucose, galactose and mannitol were the main monosaccharides in IMET1, and laminarin may be the storage polysaccharide that competes for carbon precursors with TAG. Transcriptional expression analysis revealed that the β-1,3-glucan degradation and pyruvate dehydrogenases pathways were the main regulatory components involved in driving carbon flow to TAG synthesis. Furthermore, temporal changes of lipidomes and transcripts of glycerolipid metabolism genes were indicative of possible conversion of membrane lipids to TAG, especially under an early stage of nitrogen deprivation conditions. A carbon partitioning model for N. oceanica was proposed, in which β-1,3-glucan metabolism, acetyl-CoA synthesis and membrane lipid turnover/degradation, in addition to de novo fatty acid synthesis, all contributed to TAG synthesis.

Original languageEnglish (US)
Pages (from-to)66-77
Number of pages12
JournalAlgal Research
Volume7
DOIs
StatePublished - Jan 1 2015
Externally publishedYes

Fingerprint

Nannochloropsis
triacylglycerols
carbon
nitrogen
synthesis
polysaccharides
glucans
monosaccharides
pyruvate dehydrogenase (lipoamide)
metabolism
degradation
energy
carbohydrate metabolism
lipids
mannitol
microalgae
galactose
genes
fatty acids
glucose

Keywords

  • Carbon partitioning
  • Lipidomes
  • Membrane lipids
  • Nannochloropsis
  • Storage carbohydrates

ASJC Scopus subject areas

  • Agronomy and Crop Science

Cite this

Molecular mechanisms for photosynthetic carbon partitioning into storage neutral lipids in Nannochloropsis oceanica under nitrogen-depletion conditions. / Jia, Jing; Han, Danxiang; Gerken, Henri G.; Li, Yantao; Sommerfeld, Milton; Hu, Qiang; Xu, Jian.

In: Algal Research, Vol. 7, 01.01.2015, p. 66-77.

Research output: Contribution to journalArticle

Jia, Jing ; Han, Danxiang ; Gerken, Henri G. ; Li, Yantao ; Sommerfeld, Milton ; Hu, Qiang ; Xu, Jian. / Molecular mechanisms for photosynthetic carbon partitioning into storage neutral lipids in Nannochloropsis oceanica under nitrogen-depletion conditions. In: Algal Research. 2015 ; Vol. 7. pp. 66-77.
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