TY - JOUR
T1 - Molecular identification and phylogenetic characterization of A-strain isolates of maize streak virus from western Ethiopia
AU - Ketsela, Daniel
AU - Oyeniran, Kehinde A.
AU - Feyissa, Berhanu
AU - Fontenele, Rafaela S.
AU - Kraberger, Simona
AU - Varsani, Arvind
N1 - Funding Information:
KAO was supported by the National Research Foundation (NRF) South Africa and The World Academy of Science (TWAS) Grant number 105461. The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Funding Information:
The authors would like to express sincere gratitude to Mr. Tolcha Tufa for producing the map of the surveyed areas. The facilitation and support provided by Associate Professor Darren Martin in getting samples tested at the University of Cape Town in South Africa deserve our special appreciation.
Publisher Copyright:
© 2022, The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.
PY - 2022/12
Y1 - 2022/12
N2 - The A-strain of maize streak virus (MSV) causes maize streak disease (MSD), which is a major biotic threat to maize production in sub-Saharan Africa. Previous studies have described different MSV strains of economic importance from southern and eastern African countries and how eastern African regions are hubs for MSV diversification. Despite these efforts, due to a lack of extensive sampling, there is limited knowledge about the MSV-A diversity in Ethiopia. Here, field sampling of maize plants and wild grasses with visible MSD symptoms was carried out in the western Ethiopian regions of Gambela, Oromia, and Benishangul-Gumuz during the maize-growing season of 2019. The complete genomes of MSV isolates (n = 60) were cloned and sequenced by the Sanger method. We used a model-based phylogenetic approach to analyse 725 full MSV genome sequences available in the GenBank database together with newly determined genome sequences from Ethiopia to determine their subtypes and identify recombinant lineages. Of the 127 fields accessed, MSD prevalence was highest, at 96%, in the Gambela region and lowest in Oromia, at 66%. The highest mean symptom severity of 4/5 (where 5 is the highest and 1 the lowest) was observed in Gambela and Benishangul-Gumuz. Our results show that these newly determined MSV isolates belong to recombinant lineage V of the A1 subtype, with the widest dissemination and greatest economic significance in sub-Saharan Africa and the adjacent Indian Ocean islands.
AB - The A-strain of maize streak virus (MSV) causes maize streak disease (MSD), which is a major biotic threat to maize production in sub-Saharan Africa. Previous studies have described different MSV strains of economic importance from southern and eastern African countries and how eastern African regions are hubs for MSV diversification. Despite these efforts, due to a lack of extensive sampling, there is limited knowledge about the MSV-A diversity in Ethiopia. Here, field sampling of maize plants and wild grasses with visible MSD symptoms was carried out in the western Ethiopian regions of Gambela, Oromia, and Benishangul-Gumuz during the maize-growing season of 2019. The complete genomes of MSV isolates (n = 60) were cloned and sequenced by the Sanger method. We used a model-based phylogenetic approach to analyse 725 full MSV genome sequences available in the GenBank database together with newly determined genome sequences from Ethiopia to determine their subtypes and identify recombinant lineages. Of the 127 fields accessed, MSD prevalence was highest, at 96%, in the Gambela region and lowest in Oromia, at 66%. The highest mean symptom severity of 4/5 (where 5 is the highest and 1 the lowest) was observed in Gambela and Benishangul-Gumuz. Our results show that these newly determined MSV isolates belong to recombinant lineage V of the A1 subtype, with the widest dissemination and greatest economic significance in sub-Saharan Africa and the adjacent Indian Ocean islands.
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U2 - 10.1007/s00705-022-05614-4
DO - 10.1007/s00705-022-05614-4
M3 - Article
C2 - 36169719
AN - SCOPUS:85139181313
SN - 0304-8608
VL - 167
SP - 2753
EP - 2759
JO - Archives of virology
JF - Archives of virology
IS - 12
ER -