Molecular evolution of PvMSP3a block II in Plasmodium vivax from diverse geographic origins

Bhavna Gupta, B. P Niranjan Reddy, Qi Fan, Guiyun Yan, Jeeraphat Sirichaisinthop, Jetsumon Sattabongkot, Ananias A. Escalante, Liwang Cui, Luzia Helena Carvalho

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Block II of Plasmodium vivax merozoite surface protein 3α (PvMSP3α) is conserved and has been proposed as a potential candidate for a malaria vaccine. The present study aimed to compare sequence diversity in PvMSP3a block II at a local microgeographic scale in a village as well as from larger geographic regions (countries and worldwide). Blood samples were collected from asymptomatic carriers of P. vivax in a village at the western border of Thailand and PvMSP3α was amplified and sequenced. For population genetic analysis, 237 PvMSP3α block II sequences from eleven P. vivax endemic countries were analyzed. PvMSP3α sequences from 20 village-level samples revealed two length variant types with one type containing a large deletion in block I. In contrast, block II was relatively conserved; especially, some non-synonymous mutations were extensively shared among 11 parasite populations. However, the majority of the low-frequency synonymous variations were population specific. The conserved pattern of nucleotide diversity in block II sequences was probably due to functional/structural constraints, which were further supported by the tests of neutrality. Notably, a small region in block II that encodes a predicted B cell epitope was highly polymorphic and showed signs of balancing selection, signifying that this region might be influenced by the immune selection and may serve as a starting point for designing multi-antigen/stage epitope based vaccines against this parasite.

Original languageEnglish (US)
Article numbere0135396
JournalPLoS One
Volume10
Issue number8
DOIs
StatePublished - Aug 12 2015
Externally publishedYes

Fingerprint

Plasmodium vivax
Molecular Evolution
provenance
Membrane Proteins
merozoites
surface proteins
villages
Malaria Vaccines
B-Lymphocyte Epitopes
epitopes
Parasites
parasites
Epitopes
Blood
Vaccines
Nucleotides
Population Genetics
Thailand
Antigens
Population

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Gupta, B., Reddy, B. P. N., Fan, Q., Yan, G., Sirichaisinthop, J., Sattabongkot, J., ... Carvalho, L. H. (2015). Molecular evolution of PvMSP3a block II in Plasmodium vivax from diverse geographic origins. PLoS One, 10(8), [e0135396]. https://doi.org/10.1371/journal.pone.0135396

Molecular evolution of PvMSP3a block II in Plasmodium vivax from diverse geographic origins. / Gupta, Bhavna; Reddy, B. P Niranjan; Fan, Qi; Yan, Guiyun; Sirichaisinthop, Jeeraphat; Sattabongkot, Jetsumon; Escalante, Ananias A.; Cui, Liwang; Carvalho, Luzia Helena.

In: PLoS One, Vol. 10, No. 8, e0135396, 12.08.2015.

Research output: Contribution to journalArticle

Gupta, B, Reddy, BPN, Fan, Q, Yan, G, Sirichaisinthop, J, Sattabongkot, J, Escalante, AA, Cui, L & Carvalho, LH 2015, 'Molecular evolution of PvMSP3a block II in Plasmodium vivax from diverse geographic origins', PLoS One, vol. 10, no. 8, e0135396. https://doi.org/10.1371/journal.pone.0135396
Gupta B, Reddy BPN, Fan Q, Yan G, Sirichaisinthop J, Sattabongkot J et al. Molecular evolution of PvMSP3a block II in Plasmodium vivax from diverse geographic origins. PLoS One. 2015 Aug 12;10(8). e0135396. https://doi.org/10.1371/journal.pone.0135396
Gupta, Bhavna ; Reddy, B. P Niranjan ; Fan, Qi ; Yan, Guiyun ; Sirichaisinthop, Jeeraphat ; Sattabongkot, Jetsumon ; Escalante, Ananias A. ; Cui, Liwang ; Carvalho, Luzia Helena. / Molecular evolution of PvMSP3a block II in Plasmodium vivax from diverse geographic origins. In: PLoS One. 2015 ; Vol. 10, No. 8.
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