Molecular diversity of turncurtoviruses in Iran

Mehdi Kamali, Jahangir Heydarnejad, Hossain Massumi, Anders Kvarnheden, Simona Kraberger, Arvind Varsani

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Turnip curly top virus (TCTV) is the only member of the newly established genus Turncurtovirus (family Geminiviridae). As part of an ongoing study to identify additional plant hosts and the diversity of turncurtoviruses, between 2012 and 2014, we sampled symptomatic turnip plants and other crops in the provinces Fars and Khorasan Razavi (southern and northeastern Iran, respectively). Infection by turncurtoviruses was tested by PCR and/or rolling-circle amplification (RCA) coupled with restriction enzyme digests. Turncurtoviruses were identified in turnip as well as seven other field crops, including eggplant, basil, radish, lettuce, sugar beet, red beet and spinach. Full turncurtovirus genomes were recovered from 25 of these samples, leading to the identification of TCTV and a new putative turncurtovirus, turnip leaf roll virus (TLRV; 13 isolates), which shares <80 % genome-wide pairwise identity with TCTV. Agroinoculation of plants with an infectious clone of TLRV demonstrated that this virus could infect several plant hosts under greenhouse conditions and could be transmitted by the leafhopper Circulifer haematoceps (Mulsant and Rey, 1855) from agroinoculated to healthy plants.

Original languageEnglish (US)
Pages (from-to)551-561
Number of pages11
JournalArchives of Virology
Volume161
Issue number3
DOIs
StatePublished - Mar 1 2016
Externally publishedYes

Fingerprint

Brassica napus
Iran
Viruses
Beta vulgaris
Geminiviridae
Ocimum basilicum
Solanum melongena
Genome
Raphanus
Lettuce
Hemiptera
Spinacia oleracea
Clone Cells
Polymerase Chain Reaction
Enzymes
Infection

ASJC Scopus subject areas

  • Virology
  • Medicine(all)

Cite this

Kamali, M., Heydarnejad, J., Massumi, H., Kvarnheden, A., Kraberger, S., & Varsani, A. (2016). Molecular diversity of turncurtoviruses in Iran. Archives of Virology, 161(3), 551-561. https://doi.org/10.1007/s00705-015-2686-6

Molecular diversity of turncurtoviruses in Iran. / Kamali, Mehdi; Heydarnejad, Jahangir; Massumi, Hossain; Kvarnheden, Anders; Kraberger, Simona; Varsani, Arvind.

In: Archives of Virology, Vol. 161, No. 3, 01.03.2016, p. 551-561.

Research output: Contribution to journalArticle

Kamali, M, Heydarnejad, J, Massumi, H, Kvarnheden, A, Kraberger, S & Varsani, A 2016, 'Molecular diversity of turncurtoviruses in Iran', Archives of Virology, vol. 161, no. 3, pp. 551-561. https://doi.org/10.1007/s00705-015-2686-6
Kamali M, Heydarnejad J, Massumi H, Kvarnheden A, Kraberger S, Varsani A. Molecular diversity of turncurtoviruses in Iran. Archives of Virology. 2016 Mar 1;161(3):551-561. https://doi.org/10.1007/s00705-015-2686-6
Kamali, Mehdi ; Heydarnejad, Jahangir ; Massumi, Hossain ; Kvarnheden, Anders ; Kraberger, Simona ; Varsani, Arvind. / Molecular diversity of turncurtoviruses in Iran. In: Archives of Virology. 2016 ; Vol. 161, No. 3. pp. 551-561.
@article{804985ec541247efa1ef1e0e962c23eb,
title = "Molecular diversity of turncurtoviruses in Iran",
abstract = "Turnip curly top virus (TCTV) is the only member of the newly established genus Turncurtovirus (family Geminiviridae). As part of an ongoing study to identify additional plant hosts and the diversity of turncurtoviruses, between 2012 and 2014, we sampled symptomatic turnip plants and other crops in the provinces Fars and Khorasan Razavi (southern and northeastern Iran, respectively). Infection by turncurtoviruses was tested by PCR and/or rolling-circle amplification (RCA) coupled with restriction enzyme digests. Turncurtoviruses were identified in turnip as well as seven other field crops, including eggplant, basil, radish, lettuce, sugar beet, red beet and spinach. Full turncurtovirus genomes were recovered from 25 of these samples, leading to the identification of TCTV and a new putative turncurtovirus, turnip leaf roll virus (TLRV; 13 isolates), which shares <80 {\%} genome-wide pairwise identity with TCTV. Agroinoculation of plants with an infectious clone of TLRV demonstrated that this virus could infect several plant hosts under greenhouse conditions and could be transmitted by the leafhopper Circulifer haematoceps (Mulsant and Rey, 1855) from agroinoculated to healthy plants.",
author = "Mehdi Kamali and Jahangir Heydarnejad and Hossain Massumi and Anders Kvarnheden and Simona Kraberger and Arvind Varsani",
year = "2016",
month = "3",
day = "1",
doi = "10.1007/s00705-015-2686-6",
language = "English (US)",
volume = "161",
pages = "551--561",
journal = "Archives of Virology",
issn = "0304-8608",
publisher = "Springer Wien",
number = "3",

}

TY - JOUR

T1 - Molecular diversity of turncurtoviruses in Iran

AU - Kamali, Mehdi

AU - Heydarnejad, Jahangir

AU - Massumi, Hossain

AU - Kvarnheden, Anders

AU - Kraberger, Simona

AU - Varsani, Arvind

PY - 2016/3/1

Y1 - 2016/3/1

N2 - Turnip curly top virus (TCTV) is the only member of the newly established genus Turncurtovirus (family Geminiviridae). As part of an ongoing study to identify additional plant hosts and the diversity of turncurtoviruses, between 2012 and 2014, we sampled symptomatic turnip plants and other crops in the provinces Fars and Khorasan Razavi (southern and northeastern Iran, respectively). Infection by turncurtoviruses was tested by PCR and/or rolling-circle amplification (RCA) coupled with restriction enzyme digests. Turncurtoviruses were identified in turnip as well as seven other field crops, including eggplant, basil, radish, lettuce, sugar beet, red beet and spinach. Full turncurtovirus genomes were recovered from 25 of these samples, leading to the identification of TCTV and a new putative turncurtovirus, turnip leaf roll virus (TLRV; 13 isolates), which shares <80 % genome-wide pairwise identity with TCTV. Agroinoculation of plants with an infectious clone of TLRV demonstrated that this virus could infect several plant hosts under greenhouse conditions and could be transmitted by the leafhopper Circulifer haematoceps (Mulsant and Rey, 1855) from agroinoculated to healthy plants.

AB - Turnip curly top virus (TCTV) is the only member of the newly established genus Turncurtovirus (family Geminiviridae). As part of an ongoing study to identify additional plant hosts and the diversity of turncurtoviruses, between 2012 and 2014, we sampled symptomatic turnip plants and other crops in the provinces Fars and Khorasan Razavi (southern and northeastern Iran, respectively). Infection by turncurtoviruses was tested by PCR and/or rolling-circle amplification (RCA) coupled with restriction enzyme digests. Turncurtoviruses were identified in turnip as well as seven other field crops, including eggplant, basil, radish, lettuce, sugar beet, red beet and spinach. Full turncurtovirus genomes were recovered from 25 of these samples, leading to the identification of TCTV and a new putative turncurtovirus, turnip leaf roll virus (TLRV; 13 isolates), which shares <80 % genome-wide pairwise identity with TCTV. Agroinoculation of plants with an infectious clone of TLRV demonstrated that this virus could infect several plant hosts under greenhouse conditions and could be transmitted by the leafhopper Circulifer haematoceps (Mulsant and Rey, 1855) from agroinoculated to healthy plants.

UR - http://www.scopus.com/inward/record.url?scp=84959148110&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84959148110&partnerID=8YFLogxK

U2 - 10.1007/s00705-015-2686-6

DO - 10.1007/s00705-015-2686-6

M3 - Article

VL - 161

SP - 551

EP - 561

JO - Archives of Virology

JF - Archives of Virology

SN - 0304-8608

IS - 3

ER -