Molecular cloning and sequence analysis of the penton base genes of type II avian adenoviruses

M. Suresh, S. St Cyr, J. M. Sharma

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

We describe here the identification of the penton base gene of hemorrhagic enteritis virus (HEV), a type II avian adenovirus, in a 2477-base pair (bp)-EcoRI fragment of the viral DNA by sequence analysis. Identification is based on an extensive amino acid homology between in HEV-open reading frame and the penton base of a fowl adenovirus (FAV-10) and various human adenoviruses. The 1344 bp-penton base gene of HEV encodes a 448-amino acid polypeptide of molecular weight of 50, 843 Da. The nucleotide sequences of penton base genes of HEV and marble spleen disease virus (MSDV) are identical. The HEV penton base lacks the RGD motif, present in most human adenoviruses (Ad2, Ad3, Ad4, and Ad12) suggesting that HEV may not use αv integrins to gain entry into host cells. Further sequence analysis revealed the presence of a Leu-Asp-Val (LDV) motif in the HEV penton base amino acid sequence similar to most of the human adenoviruses. LDV motif on the fibronectin has been shown to interact with the α4β1 integrins on cells, which includes lymphocytes and monocytes. The presence of LDV motif in the penton base of HEV implicates the involvement of α4β1 integrins in the viral internalization into host cells.

Original languageEnglish (US)
Pages (from-to)289-297
Number of pages9
JournalVirus research
Volume39
Issue number2-3
DOIs
StatePublished - Dec 1995

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Keywords

  • Gene
  • Hemorrhagic enteritis virus
  • LDV motif
  • Penton base
  • Type II avian adenovirus

ASJC Scopus subject areas

  • Cancer Research
  • Virology
  • Infectious Diseases

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