Molecular characterisation of a novel cassava associated circular ssDNA virus

Anisha Dayaram, Allen Opong, Anja Jäschke, James Hadfield, Marianna Baschiera, Renwick C J Dobson, Samuel K. Offei, Dionne N. Shepherd, Darren P. Martin, Arvind Varsani

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

The application of sequence non-specific rolling circle amplification of circular single stranded (ss) DNA molecules to viral metagenomics has facilitated the discovery in various ecosystems of what is probably a diverse array of novel ssDNA viruses. Here we describe a putative novel ssDNA virus (at a genome level), cassava associated circular DNA virus (CasCV), isolated from cassava leaf samples infected with the fungi Collectotrichum and Plectosphaerella. CasCV has a circular ambisense genome and shares significant genome similarities with Sclerotinia sclerotiorum hypovirulence-associated DNA virus 1 (SsHADV-1), Mosquito VEM virus SDBVL and Meles meles faecal virus (MmFV). The CasCV genome (2220. nt) has three large open reading frames. While it is probable that one of these encodes a capsid protein, the other two probably express a replication associated protein (Rep) following the removal of an intron such as that found in the Rep encoding genes of some geminiviruses. This Rep would contain four conserved rolling circle replication (RCR) related motifs that have previously been identified in geminivirus, circovirus and nanovirus Reps. Given both that the CasCV Rep and CP share 62.7% and 39.8% amino acid identity respectively with the Rep and CP of SsHADV-1, and that CasCV was discovered associated with cassava infecting fungi, we suggest that CasCV should be classified within the mycovirus taxonomic family. However, host range studies using infectious clones will be required to demonstrate the novel virus' likely origin and actual host species.

Original languageEnglish (US)
Pages (from-to)130-135
Number of pages6
JournalVirus Research
Volume166
Issue number1-2
DOIs
StatePublished - Jun 2012
Externally publishedYes

Fingerprint

Manihot
DNA Viruses
Circular DNA
Viruses
Geminiviridae
Genome
Satellite Viruses
Ascomycota
Proteins
Nanovirus
Fungi
Circovirus
Metagenomics
Host Specificity
Single-Stranded DNA
Capsid Proteins
Virus Replication
Culicidae
DNA Replication
Introns

Keywords

  • Cassava
  • Rolling circle amplification
  • SsDNA virus

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases
  • Cancer Research

Cite this

Dayaram, A., Opong, A., Jäschke, A., Hadfield, J., Baschiera, M., Dobson, R. C. J., ... Varsani, A. (2012). Molecular characterisation of a novel cassava associated circular ssDNA virus. Virus Research, 166(1-2), 130-135. https://doi.org/10.1016/j.virusres.2012.03.009

Molecular characterisation of a novel cassava associated circular ssDNA virus. / Dayaram, Anisha; Opong, Allen; Jäschke, Anja; Hadfield, James; Baschiera, Marianna; Dobson, Renwick C J; Offei, Samuel K.; Shepherd, Dionne N.; Martin, Darren P.; Varsani, Arvind.

In: Virus Research, Vol. 166, No. 1-2, 06.2012, p. 130-135.

Research output: Contribution to journalArticle

Dayaram, A, Opong, A, Jäschke, A, Hadfield, J, Baschiera, M, Dobson, RCJ, Offei, SK, Shepherd, DN, Martin, DP & Varsani, A 2012, 'Molecular characterisation of a novel cassava associated circular ssDNA virus', Virus Research, vol. 166, no. 1-2, pp. 130-135. https://doi.org/10.1016/j.virusres.2012.03.009
Dayaram A, Opong A, Jäschke A, Hadfield J, Baschiera M, Dobson RCJ et al. Molecular characterisation of a novel cassava associated circular ssDNA virus. Virus Research. 2012 Jun;166(1-2):130-135. https://doi.org/10.1016/j.virusres.2012.03.009
Dayaram, Anisha ; Opong, Allen ; Jäschke, Anja ; Hadfield, James ; Baschiera, Marianna ; Dobson, Renwick C J ; Offei, Samuel K. ; Shepherd, Dionne N. ; Martin, Darren P. ; Varsani, Arvind. / Molecular characterisation of a novel cassava associated circular ssDNA virus. In: Virus Research. 2012 ; Vol. 166, No. 1-2. pp. 130-135.
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