Molecular Chaperones of the Endoplasmic Reticulum Promote Hepatitis C Virus E2 Protein Production in Plants

Hugh Mason (Inventor)

Research output: Patent

Abstract

Infections caused by the Hepatitis C virus (HCV) are very common worldwide (afflicting up to 3% of the population), and chronic infection may develop into cirrhosis and liver cancer. For this reason a vaccine against HCV would have great utility, with the HCV envelope protein 2 (E2) a promising candidate because it can directly bind to a human cell receptor and plays a role in viral entry. However, studies of producing E2 for a vaccine have shown that much of the E2 produced is misfolded and thus not functional. Researchers at the Biodesign Institute of Arizona State University have developed a method to transiently overexpress two molecular chaperones in the endoplasmic reticulum of plants in order to facilitate E2 folding and production. Western blot analysis showed that calnexin and calreticulin overexpression in Nicotiana benthamiana significantly increased the yield of properly-folded E2 compared to controls. This discovery has the potential to accelerate the research for a commercially-viable vaccine for Hepatitis C virus. Potential Applications therapeutic for Hepatitis C virus prophylactic vaccine for Hepatitis C virus Benefits and Advantages increases the yield of the promising vaccine candidate E2 amenable to use in a plant system, with the attendant advantages of greater safety, lower cost and easier scalability to industrial production Dowload Original PDF For more information about the inventor(s) and their research, please see Dr. Mason's directory webpage Dr. Mason's departmental webpage
Original languageEnglish (US)
StatePublished - May 2 2011

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Molecular Chaperones
Hepacivirus
Endoplasmic Reticulum
Vaccines
Proteins
Inventors
Calnexin
Calreticulin
Directories
Protein Folding
Liver Neoplasms
Infection
Research
Tobacco
Fibrosis
Western Blotting
Research Personnel
Hepatitis C virus glycoprotein E2
Safety
Costs and Cost Analysis

Cite this

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title = "Molecular Chaperones of the Endoplasmic Reticulum Promote Hepatitis C Virus E2 Protein Production in Plants",
abstract = "Infections caused by the Hepatitis C virus (HCV) are very common worldwide (afflicting up to 3{\%} of the population), and chronic infection may develop into cirrhosis and liver cancer. For this reason a vaccine against HCV would have great utility, with the HCV envelope protein 2 (E2) a promising candidate because it can directly bind to a human cell receptor and plays a role in viral entry. However, studies of producing E2 for a vaccine have shown that much of the E2 produced is misfolded and thus not functional. Researchers at the Biodesign Institute of Arizona State University have developed a method to transiently overexpress two molecular chaperones in the endoplasmic reticulum of plants in order to facilitate E2 folding and production. Western blot analysis showed that calnexin and calreticulin overexpression in Nicotiana benthamiana significantly increased the yield of properly-folded E2 compared to controls. This discovery has the potential to accelerate the research for a commercially-viable vaccine for Hepatitis C virus. Potential Applications therapeutic for Hepatitis C virus prophylactic vaccine for Hepatitis C virus Benefits and Advantages increases the yield of the promising vaccine candidate E2 amenable to use in a plant system, with the attendant advantages of greater safety, lower cost and easier scalability to industrial production Dowload Original PDF For more information about the inventor(s) and their research, please see Dr. Mason's directory webpage Dr. Mason's departmental webpage",
author = "Hugh Mason",
year = "2011",
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type = "Patent",

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T1 - Molecular Chaperones of the Endoplasmic Reticulum Promote Hepatitis C Virus E2 Protein Production in Plants

AU - Mason, Hugh

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N2 - Infections caused by the Hepatitis C virus (HCV) are very common worldwide (afflicting up to 3% of the population), and chronic infection may develop into cirrhosis and liver cancer. For this reason a vaccine against HCV would have great utility, with the HCV envelope protein 2 (E2) a promising candidate because it can directly bind to a human cell receptor and plays a role in viral entry. However, studies of producing E2 for a vaccine have shown that much of the E2 produced is misfolded and thus not functional. Researchers at the Biodesign Institute of Arizona State University have developed a method to transiently overexpress two molecular chaperones in the endoplasmic reticulum of plants in order to facilitate E2 folding and production. Western blot analysis showed that calnexin and calreticulin overexpression in Nicotiana benthamiana significantly increased the yield of properly-folded E2 compared to controls. This discovery has the potential to accelerate the research for a commercially-viable vaccine for Hepatitis C virus. Potential Applications therapeutic for Hepatitis C virus prophylactic vaccine for Hepatitis C virus Benefits and Advantages increases the yield of the promising vaccine candidate E2 amenable to use in a plant system, with the attendant advantages of greater safety, lower cost and easier scalability to industrial production Dowload Original PDF For more information about the inventor(s) and their research, please see Dr. Mason's directory webpage Dr. Mason's departmental webpage

AB - Infections caused by the Hepatitis C virus (HCV) are very common worldwide (afflicting up to 3% of the population), and chronic infection may develop into cirrhosis and liver cancer. For this reason a vaccine against HCV would have great utility, with the HCV envelope protein 2 (E2) a promising candidate because it can directly bind to a human cell receptor and plays a role in viral entry. However, studies of producing E2 for a vaccine have shown that much of the E2 produced is misfolded and thus not functional. Researchers at the Biodesign Institute of Arizona State University have developed a method to transiently overexpress two molecular chaperones in the endoplasmic reticulum of plants in order to facilitate E2 folding and production. Western blot analysis showed that calnexin and calreticulin overexpression in Nicotiana benthamiana significantly increased the yield of properly-folded E2 compared to controls. This discovery has the potential to accelerate the research for a commercially-viable vaccine for Hepatitis C virus. Potential Applications therapeutic for Hepatitis C virus prophylactic vaccine for Hepatitis C virus Benefits and Advantages increases the yield of the promising vaccine candidate E2 amenable to use in a plant system, with the attendant advantages of greater safety, lower cost and easier scalability to industrial production Dowload Original PDF For more information about the inventor(s) and their research, please see Dr. Mason's directory webpage Dr. Mason's departmental webpage

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