Molecular and partial biological characterization of the coat protein sequences of Iranian alfalfa mosaic virus isolates

Fatemeh Mangeli, Hossain Massumi, Fereshteh Alipour, Mohammad Maddahian, Jahangir Heydarnejad, Akbar Hosseinipour, Mohammad Hadi Amid-Motlagh, Morteza Azizizadeh, Arvind Varsani

Research output: Contribution to journalArticle

Abstract

Alfalfa mosaic virus (AMV) is one of the most important viruses that cause significant losses to alfalfa worldwide. A total of 1066 symptomatic alfalfa, 143 weeds and other secondary hosts were collected and assayed for AMV infection. The virus was detected in 360 samples (33.8% incidence) of alfalfa by plate-trapped antigen (PTA)-ELISA. Among the weed species and the other plants tested, potato, pepper and seven weed species were infected with AMV. The coat protein (CP) gene sequences from 59 Iranian isolates were PCR amplified, cloned, sequenced, and compared with the sequences available in GenBank. Sequence analysis of the amplified regions showed that 55 of the Iranian CP sequences share >98% pairwise identity with each other. The remaining four share between 89 and 96% identity. Our host studies indicated that the Iranian AMV isolates were classified into two groups. Only four Iranian isolates were clustered in the symptom-group B and caused relatively milder symptoms compared to those found in symptom-group A.

Original languageEnglish (US)
Pages (from-to)735-742
Number of pages8
JournalJournal of Plant Pathology
Volume101
Issue number3
DOIs
StatePublished - Aug 15 2019

Fingerprint

Alfalfa mosaic virus
coat proteins
amino acid sequences
signs and symptoms (plants)
alfalfa
weeds
viruses
intermediate hosts
pepper
sequence analysis
enzyme-linked immunosorbent assay
potatoes
antigens
nucleotide sequences
incidence
infection
sampling

Keywords

  • Alfalfa
  • Alfalfa mosaic virus
  • Bromoviridae
  • Iran

ASJC Scopus subject areas

  • Plant Science

Cite this

Molecular and partial biological characterization of the coat protein sequences of Iranian alfalfa mosaic virus isolates. / Mangeli, Fatemeh; Massumi, Hossain; Alipour, Fereshteh; Maddahian, Mohammad; Heydarnejad, Jahangir; Hosseinipour, Akbar; Amid-Motlagh, Mohammad Hadi; Azizizadeh, Morteza; Varsani, Arvind.

In: Journal of Plant Pathology, Vol. 101, No. 3, 15.08.2019, p. 735-742.

Research output: Contribution to journalArticle

Mangeli, F, Massumi, H, Alipour, F, Maddahian, M, Heydarnejad, J, Hosseinipour, A, Amid-Motlagh, MH, Azizizadeh, M & Varsani, A 2019, 'Molecular and partial biological characterization of the coat protein sequences of Iranian alfalfa mosaic virus isolates', Journal of Plant Pathology, vol. 101, no. 3, pp. 735-742. https://doi.org/10.1007/s42161-019-00275-w
Mangeli, Fatemeh ; Massumi, Hossain ; Alipour, Fereshteh ; Maddahian, Mohammad ; Heydarnejad, Jahangir ; Hosseinipour, Akbar ; Amid-Motlagh, Mohammad Hadi ; Azizizadeh, Morteza ; Varsani, Arvind. / Molecular and partial biological characterization of the coat protein sequences of Iranian alfalfa mosaic virus isolates. In: Journal of Plant Pathology. 2019 ; Vol. 101, No. 3. pp. 735-742.
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