Modulation of fluorescence in Heliobacterium modesticaldum cells

Aaron M. Collins, Kevin Redding, Robert E. Blankenship

    Research output: Contribution to journalArticle

    5 Citations (Scopus)

    Abstract

    In what appears to be a common theme for all phototrophs, heliobacteria exhibit complex modulations of fluorescence yield when illuminated with actinic light and probed on a time scale of μs to minutes. The fluorescence yield from cells of Heliobacterium modesticaldum remained nearly constant for the first 10-100 ms of illumination and then rose to a maximum level with one or two inflections over the course of many seconds. Fluorescence then declined to a steady-state value within about one minute. In this analysis, the origins of the fluorescence induction in whole cells of heliobacteria are investigated by treating cells with a combination of electron accepters, donors, and inhibitors of the photosynthetic electron transport, as well as varying the temperature. We conclude that fluorescence modulation in H. modesticaldum results from acceptor-side limitation in the reaction center (RC), possibly due to charge recombination between P800 + and A0 -.

    Original languageEnglish (US)
    Pages (from-to)283-292
    Number of pages10
    JournalPhotosynthesis Research
    Volume104
    Issue number2
    DOIs
    StatePublished - Jun 2010

    Fingerprint

    Heliobacterium
    Fluorescence
    Modulation
    fluorescence
    Heliobacteriaceae
    cells
    autotrophs
    Electron Transport
    Lighting
    Genetic Recombination
    electron transfer
    lighting
    Rosa
    electrons
    Electrons
    Light
    Temperature

    Keywords

    • Fluorescence induction
    • Fluorescence modulation
    • Heliobacterium modesticaldum

    ASJC Scopus subject areas

    • Plant Science
    • Cell Biology
    • Biochemistry

    Cite this

    Modulation of fluorescence in Heliobacterium modesticaldum cells. / Collins, Aaron M.; Redding, Kevin; Blankenship, Robert E.

    In: Photosynthesis Research, Vol. 104, No. 2, 06.2010, p. 283-292.

    Research output: Contribution to journalArticle

    Collins, Aaron M. ; Redding, Kevin ; Blankenship, Robert E. / Modulation of fluorescence in Heliobacterium modesticaldum cells. In: Photosynthesis Research. 2010 ; Vol. 104, No. 2. pp. 283-292.
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