Abstract
In what appears to be a common theme for all phototrophs, heliobacteria exhibit complex modulations of fluorescence yield when illuminated with actinic light and probed on a time scale of μs to minutes. The fluorescence yield from cells of Heliobacterium modesticaldum remained nearly constant for the first 10-100 ms of illumination and then rose to a maximum level with one or two inflections over the course of many seconds. Fluorescence then declined to a steady-state value within about one minute. In this analysis, the origins of the fluorescence induction in whole cells of heliobacteria are investigated by treating cells with a combination of electron accepters, donors, and inhibitors of the photosynthetic electron transport, as well as varying the temperature. We conclude that fluorescence modulation in H. modesticaldum results from acceptor-side limitation in the reaction center (RC), possibly due to charge recombination between P800 + and A0 -.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 283-292 |
| Number of pages | 10 |
| Journal | Photosynthesis Research |
| Volume | 104 |
| Issue number | 2 |
| DOIs | |
| State | Published - Jun 2010 |
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Keywords
- Fluorescence induction
- Fluorescence modulation
- Heliobacterium modesticaldum
ASJC Scopus subject areas
- Plant Science
- Cell Biology
- Biochemistry
Cite this
Modulation of fluorescence in Heliobacterium modesticaldum cells. / Collins, Aaron M.; Redding, Kevin; Blankenship, Robert E.
In: Photosynthesis Research, Vol. 104, No. 2, 06.2010, p. 283-292.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Modulation of fluorescence in Heliobacterium modesticaldum cells
AU - Collins, Aaron M.
AU - Redding, Kevin
AU - Blankenship, Robert E.
PY - 2010/6
Y1 - 2010/6
N2 - In what appears to be a common theme for all phototrophs, heliobacteria exhibit complex modulations of fluorescence yield when illuminated with actinic light and probed on a time scale of μs to minutes. The fluorescence yield from cells of Heliobacterium modesticaldum remained nearly constant for the first 10-100 ms of illumination and then rose to a maximum level with one or two inflections over the course of many seconds. Fluorescence then declined to a steady-state value within about one minute. In this analysis, the origins of the fluorescence induction in whole cells of heliobacteria are investigated by treating cells with a combination of electron accepters, donors, and inhibitors of the photosynthetic electron transport, as well as varying the temperature. We conclude that fluorescence modulation in H. modesticaldum results from acceptor-side limitation in the reaction center (RC), possibly due to charge recombination between P800 + and A0 -.
AB - In what appears to be a common theme for all phototrophs, heliobacteria exhibit complex modulations of fluorescence yield when illuminated with actinic light and probed on a time scale of μs to minutes. The fluorescence yield from cells of Heliobacterium modesticaldum remained nearly constant for the first 10-100 ms of illumination and then rose to a maximum level with one or two inflections over the course of many seconds. Fluorescence then declined to a steady-state value within about one minute. In this analysis, the origins of the fluorescence induction in whole cells of heliobacteria are investigated by treating cells with a combination of electron accepters, donors, and inhibitors of the photosynthetic electron transport, as well as varying the temperature. We conclude that fluorescence modulation in H. modesticaldum results from acceptor-side limitation in the reaction center (RC), possibly due to charge recombination between P800 + and A0 -.
KW - Fluorescence induction
KW - Fluorescence modulation
KW - Heliobacterium modesticaldum
UR - http://www.scopus.com/inward/record.url?scp=77953650684&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77953650684&partnerID=8YFLogxK
U2 - 10.1007/s11120-010-9554-8
DO - 10.1007/s11120-010-9554-8
M3 - Article
C2 - 20461555
AN - SCOPUS:77953650684
VL - 104
SP - 283
EP - 292
JO - Photosynthesis Research
JF - Photosynthesis Research
SN - 0166-8595
IS - 2
ER -