Modulation of fluorescence in Heliobacterium modesticaldum cells

Aaron M. Collins, Kevin Redding, Robert E. Blankenship

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

In what appears to be a common theme for all phototrophs, heliobacteria exhibit complex modulations of fluorescence yield when illuminated with actinic light and probed on a time scale of μs to minutes. The fluorescence yield from cells of Heliobacterium modesticaldum remained nearly constant for the first 10-100 ms of illumination and then rose to a maximum level with one or two inflections over the course of many seconds. Fluorescence then declined to a steady-state value within about one minute. In this analysis, the origins of the fluorescence induction in whole cells of heliobacteria are investigated by treating cells with a combination of electron accepters, donors, and inhibitors of the photosynthetic electron transport, as well as varying the temperature. We conclude that fluorescence modulation in H. modesticaldum results from acceptor-side limitation in the reaction center (RC), possibly due to charge recombination between P800 + and A0 -.

Original languageEnglish (US)
Pages (from-to)283-292
Number of pages10
JournalPhotosynthesis Research
Volume104
Issue number2
DOIs
StatePublished - Jun 2010

Fingerprint

Heliobacterium
Fluorescence
Modulation
fluorescence
Heliobacteriaceae
cells
autotrophs
Electron Transport
Lighting
Genetic Recombination
electron transfer
lighting
Rosa
electrons
Electrons
Light
Temperature

Keywords

  • Fluorescence induction
  • Fluorescence modulation
  • Heliobacterium modesticaldum

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology
  • Biochemistry

Cite this

Modulation of fluorescence in Heliobacterium modesticaldum cells. / Collins, Aaron M.; Redding, Kevin; Blankenship, Robert E.

In: Photosynthesis Research, Vol. 104, No. 2, 06.2010, p. 283-292.

Research output: Contribution to journalArticle

Collins, Aaron M. ; Redding, Kevin ; Blankenship, Robert E. / Modulation of fluorescence in Heliobacterium modesticaldum cells. In: Photosynthesis Research. 2010 ; Vol. 104, No. 2. pp. 283-292.
@article{c5376234bec54c0a9dfce359fcd8ae47,
title = "Modulation of fluorescence in Heliobacterium modesticaldum cells",
abstract = "In what appears to be a common theme for all phototrophs, heliobacteria exhibit complex modulations of fluorescence yield when illuminated with actinic light and probed on a time scale of μs to minutes. The fluorescence yield from cells of Heliobacterium modesticaldum remained nearly constant for the first 10-100 ms of illumination and then rose to a maximum level with one or two inflections over the course of many seconds. Fluorescence then declined to a steady-state value within about one minute. In this analysis, the origins of the fluorescence induction in whole cells of heliobacteria are investigated by treating cells with a combination of electron accepters, donors, and inhibitors of the photosynthetic electron transport, as well as varying the temperature. We conclude that fluorescence modulation in H. modesticaldum results from acceptor-side limitation in the reaction center (RC), possibly due to charge recombination between P800 + and A0 -.",
keywords = "Fluorescence induction, Fluorescence modulation, Heliobacterium modesticaldum",
author = "Collins, {Aaron M.} and Kevin Redding and Blankenship, {Robert E.}",
year = "2010",
month = "6",
doi = "10.1007/s11120-010-9554-8",
language = "English (US)",
volume = "104",
pages = "283--292",
journal = "Photosynthesis Research",
issn = "0166-8595",
publisher = "Springer Netherlands",
number = "2",

}

TY - JOUR

T1 - Modulation of fluorescence in Heliobacterium modesticaldum cells

AU - Collins, Aaron M.

AU - Redding, Kevin

AU - Blankenship, Robert E.

PY - 2010/6

Y1 - 2010/6

N2 - In what appears to be a common theme for all phototrophs, heliobacteria exhibit complex modulations of fluorescence yield when illuminated with actinic light and probed on a time scale of μs to minutes. The fluorescence yield from cells of Heliobacterium modesticaldum remained nearly constant for the first 10-100 ms of illumination and then rose to a maximum level with one or two inflections over the course of many seconds. Fluorescence then declined to a steady-state value within about one minute. In this analysis, the origins of the fluorescence induction in whole cells of heliobacteria are investigated by treating cells with a combination of electron accepters, donors, and inhibitors of the photosynthetic electron transport, as well as varying the temperature. We conclude that fluorescence modulation in H. modesticaldum results from acceptor-side limitation in the reaction center (RC), possibly due to charge recombination between P800 + and A0 -.

AB - In what appears to be a common theme for all phototrophs, heliobacteria exhibit complex modulations of fluorescence yield when illuminated with actinic light and probed on a time scale of μs to minutes. The fluorescence yield from cells of Heliobacterium modesticaldum remained nearly constant for the first 10-100 ms of illumination and then rose to a maximum level with one or two inflections over the course of many seconds. Fluorescence then declined to a steady-state value within about one minute. In this analysis, the origins of the fluorescence induction in whole cells of heliobacteria are investigated by treating cells with a combination of electron accepters, donors, and inhibitors of the photosynthetic electron transport, as well as varying the temperature. We conclude that fluorescence modulation in H. modesticaldum results from acceptor-side limitation in the reaction center (RC), possibly due to charge recombination between P800 + and A0 -.

KW - Fluorescence induction

KW - Fluorescence modulation

KW - Heliobacterium modesticaldum

UR - http://www.scopus.com/inward/record.url?scp=77953650684&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77953650684&partnerID=8YFLogxK

U2 - 10.1007/s11120-010-9554-8

DO - 10.1007/s11120-010-9554-8

M3 - Article

VL - 104

SP - 283

EP - 292

JO - Photosynthesis Research

JF - Photosynthesis Research

SN - 0166-8595

IS - 2

ER -