Modulating fibroblast adhesion, spreading, and proliferation using self- assembled monolayer films of alkylthiolates on gold

Kristin B. McClary, Tatiana Ugarova, David W. Grainger

Research output: Contribution to journalArticle

185 Citations (Scopus)

Abstract

Ultrathin, highly organized functionalized alkylthiol monolayers were applied as model substrates for cell growth and protein adsorption studies. The aim of this approach was to improve the understanding of molecular surface determinants required for adhesion-dependent cell growth and proliferation using well-controlled surface chemistry. Carboxyl- and methyl- terminated alkylthiol monolayers on gold were used to monitor Swiss 3T3 fibroblast adhesion, spreading, and growth. Stress fiber and focal contact formation were determined by immunostaining of actin filaments and paxillin. Fibronectin deposition and conformation on these surface chemistries in the presence and absence of competing proteins were also determined. The relative levels of adsorbed fibronectin were assessed using radiolabeled proteins. Exposure of the 10th type III cell integrin binding domain of fibronectin was assessed using a radiolabeled monoclonal antibody. Distinct alkyithiol substrate chemistry-dependent differences were observed in fibroblast adhesion, spreading, and growth. The formation of focal contacts and stress fibers was enhanced on the carboxyl-terminated surface relative to the methyl surface. Relative deposition and conformations of adsorbed fibronectin were shown to be dependent on surface chemistry in both the presence and absence of competing proteins. The results indicated that well-controlled culture surfaces modulate differential cell adhesion, spreading, and growth through modulations of the amounts and conformations of adsorbed extracellular matrix molecules (e.g., fibronectin). (C) 2000 John Wiley and Sons, Inc.

Original languageEnglish (US)
Pages (from-to)428-439
Number of pages12
JournalJournal of Biomedical Materials Research
Volume50
Issue number3
DOIs
StatePublished - 2000
Externally publishedYes

Fingerprint

Self assembled monolayers
Fibroblasts
Fibronectins
Gold
Cell growth
Adhesion
Surface chemistry
Proteins
Conformations
Monolayers
Monoclonal antibodies
Fibers
Cell adhesion
Paxillin
Cell proliferation
Substrates
Cell culture
Integrins
Modulation
Actins

Keywords

  • Actin
  • AlkylthioIs
  • Attachment
  • Cell adhesion
  • Cell culture
  • Fibronectin
  • Self-assembled monolayers
  • Surface chemistry

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials

Cite this

Modulating fibroblast adhesion, spreading, and proliferation using self- assembled monolayer films of alkylthiolates on gold. / McClary, Kristin B.; Ugarova, Tatiana; Grainger, David W.

In: Journal of Biomedical Materials Research, Vol. 50, No. 3, 2000, p. 428-439.

Research output: Contribution to journalArticle

@article{a72b8276e9ad4375b623286ffdbfffb8,
title = "Modulating fibroblast adhesion, spreading, and proliferation using self- assembled monolayer films of alkylthiolates on gold",
abstract = "Ultrathin, highly organized functionalized alkylthiol monolayers were applied as model substrates for cell growth and protein adsorption studies. The aim of this approach was to improve the understanding of molecular surface determinants required for adhesion-dependent cell growth and proliferation using well-controlled surface chemistry. Carboxyl- and methyl- terminated alkylthiol monolayers on gold were used to monitor Swiss 3T3 fibroblast adhesion, spreading, and growth. Stress fiber and focal contact formation were determined by immunostaining of actin filaments and paxillin. Fibronectin deposition and conformation on these surface chemistries in the presence and absence of competing proteins were also determined. The relative levels of adsorbed fibronectin were assessed using radiolabeled proteins. Exposure of the 10th type III cell integrin binding domain of fibronectin was assessed using a radiolabeled monoclonal antibody. Distinct alkyithiol substrate chemistry-dependent differences were observed in fibroblast adhesion, spreading, and growth. The formation of focal contacts and stress fibers was enhanced on the carboxyl-terminated surface relative to the methyl surface. Relative deposition and conformations of adsorbed fibronectin were shown to be dependent on surface chemistry in both the presence and absence of competing proteins. The results indicated that well-controlled culture surfaces modulate differential cell adhesion, spreading, and growth through modulations of the amounts and conformations of adsorbed extracellular matrix molecules (e.g., fibronectin). (C) 2000 John Wiley and Sons, Inc.",
keywords = "Actin, AlkylthioIs, Attachment, Cell adhesion, Cell culture, Fibronectin, Self-assembled monolayers, Surface chemistry",
author = "McClary, {Kristin B.} and Tatiana Ugarova and Grainger, {David W.}",
year = "2000",
doi = "10.1002/(SICI)1097-4636(20000605)50:3<428::AID-JBM18>3.0.CO;2-H",
language = "English (US)",
volume = "50",
pages = "428--439",
journal = "Journal of Biomedical Materials Research - Part B Applied Biomaterials",
issn = "0021-9304",
publisher = "Heterocorporation",
number = "3",

}

TY - JOUR

T1 - Modulating fibroblast adhesion, spreading, and proliferation using self- assembled monolayer films of alkylthiolates on gold

AU - McClary, Kristin B.

AU - Ugarova, Tatiana

AU - Grainger, David W.

PY - 2000

Y1 - 2000

N2 - Ultrathin, highly organized functionalized alkylthiol monolayers were applied as model substrates for cell growth and protein adsorption studies. The aim of this approach was to improve the understanding of molecular surface determinants required for adhesion-dependent cell growth and proliferation using well-controlled surface chemistry. Carboxyl- and methyl- terminated alkylthiol monolayers on gold were used to monitor Swiss 3T3 fibroblast adhesion, spreading, and growth. Stress fiber and focal contact formation were determined by immunostaining of actin filaments and paxillin. Fibronectin deposition and conformation on these surface chemistries in the presence and absence of competing proteins were also determined. The relative levels of adsorbed fibronectin were assessed using radiolabeled proteins. Exposure of the 10th type III cell integrin binding domain of fibronectin was assessed using a radiolabeled monoclonal antibody. Distinct alkyithiol substrate chemistry-dependent differences were observed in fibroblast adhesion, spreading, and growth. The formation of focal contacts and stress fibers was enhanced on the carboxyl-terminated surface relative to the methyl surface. Relative deposition and conformations of adsorbed fibronectin were shown to be dependent on surface chemistry in both the presence and absence of competing proteins. The results indicated that well-controlled culture surfaces modulate differential cell adhesion, spreading, and growth through modulations of the amounts and conformations of adsorbed extracellular matrix molecules (e.g., fibronectin). (C) 2000 John Wiley and Sons, Inc.

AB - Ultrathin, highly organized functionalized alkylthiol monolayers were applied as model substrates for cell growth and protein adsorption studies. The aim of this approach was to improve the understanding of molecular surface determinants required for adhesion-dependent cell growth and proliferation using well-controlled surface chemistry. Carboxyl- and methyl- terminated alkylthiol monolayers on gold were used to monitor Swiss 3T3 fibroblast adhesion, spreading, and growth. Stress fiber and focal contact formation were determined by immunostaining of actin filaments and paxillin. Fibronectin deposition and conformation on these surface chemistries in the presence and absence of competing proteins were also determined. The relative levels of adsorbed fibronectin were assessed using radiolabeled proteins. Exposure of the 10th type III cell integrin binding domain of fibronectin was assessed using a radiolabeled monoclonal antibody. Distinct alkyithiol substrate chemistry-dependent differences were observed in fibroblast adhesion, spreading, and growth. The formation of focal contacts and stress fibers was enhanced on the carboxyl-terminated surface relative to the methyl surface. Relative deposition and conformations of adsorbed fibronectin were shown to be dependent on surface chemistry in both the presence and absence of competing proteins. The results indicated that well-controlled culture surfaces modulate differential cell adhesion, spreading, and growth through modulations of the amounts and conformations of adsorbed extracellular matrix molecules (e.g., fibronectin). (C) 2000 John Wiley and Sons, Inc.

KW - Actin

KW - AlkylthioIs

KW - Attachment

KW - Cell adhesion

KW - Cell culture

KW - Fibronectin

KW - Self-assembled monolayers

KW - Surface chemistry

UR - http://www.scopus.com/inward/record.url?scp=0034082752&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034082752&partnerID=8YFLogxK

U2 - 10.1002/(SICI)1097-4636(20000605)50:3<428::AID-JBM18>3.0.CO;2-H

DO - 10.1002/(SICI)1097-4636(20000605)50:3<428::AID-JBM18>3.0.CO;2-H

M3 - Article

C2 - 10737886

AN - SCOPUS:0034082752

VL - 50

SP - 428

EP - 439

JO - Journal of Biomedical Materials Research - Part B Applied Biomaterials

JF - Journal of Biomedical Materials Research - Part B Applied Biomaterials

SN - 0021-9304

IS - 3

ER -