Modification of the interaction between Photosystem II and the light-harvesting chlorophyll a b-protein complex by protein phosphorylation in developing wheat thylakoids exhibiting different degrees of lateral heterogeneity

Michael P. Percival, Andrew N. Webber, John P. Markwell, Neil R. Baker

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

The effects of phosphorylation of thylakoid polypeptides at 2 and 5 mM Mg2+ on the chlorophyll fluorescence induction characteristics of developing wheat thylakoids, exhibiting differing degrees of lateral heterogeneity of intrinsic protein complexes, were studied. The relative contributions of the fast and slow components of Fv, defined by βmax, were the same at all stages of thylakoid development. On phosphorylation at 5 mM Mg2+, βmax increased similarly at all stages of thylakoid development. Such changes in βmax indicate that the magnitude of phosphorylation-induced disconnection of the light-harvesting chlorophyll a b protein complex from Photosystem II was independent of the degree of lateral heterogeneity of the thylakoid membranes. At 2 mM Mg2+ phosphorylation also increased βmax at all developmental stages, but to a lesser extent than was observed at 5 mM Mg2+. Discussion is made of how changes in the lateral heterogeneity of the pigment-protein complexes within the thylakoid that occur during membrane development and on reduction of Mg2+ concentration can modify the effects of protein phosphorylation on the energetic interactions between the complexes.

Original languageEnglish (US)
Pages (from-to)317-323
Number of pages7
JournalBBA - Bioenergetics
Volume848
Issue number3
DOIs
StatePublished - Mar 12 1986
Externally publishedYes

Keywords

  • Chlorophyll fluorescence kinetics
  • Chlorophyll-protein complex
  • Chloroplast development
  • Light-harvesting complex
  • Protein phosphorylation
  • Wheat

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Cell Biology

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