MODELING, DESIGN AND FABRICATION OF A MICRODEVICE FOR E-FIELD CELL LYSIS

Thomas Smekal; Cindy Briscoe; Tony Chan; Yung Chang; Seth Dobrin; Sean Gallagher; Heidi Groninger; Rob Marrero; David Rhine; Huinan Yu; Piotr Grodzinski

doi:10.1115/IMECE1999-0273

MODELING, DESIGN AND FABRICATION OF A MICRODEVICE FOR E-FIELD CELL LYSIS

Thomas Smekal, Cindy Briscoe, Tony Chan, Yung Chang, Seth Dobrin, Sean Gallagher, Heidi Groninger, Rob Marrero, David Rhine, Huinan Yu, Piotr Grodzinski

Life Sciences, School of (SOLS)

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

The development of an electric field cell lysis device for microfluidic applications will be presented. Detailed modeling of the electrode configuration for the optimization of the electric field has been performed. Device design and fabrication has been accomplished using traditional integrated circuit (IC) manufacturing techniques. As a result, murine lymphocytes have been lysed using several electrode configurations. The requisite electric field strength and pulse parameters for cell lysis were determined. The non-specific adsorption of the released DNA to the materials used in the fabrication of the device was observed. Surface passivation of the fabrication materials was effected through the use of silanization compounds.

Original language	English (US)
Title of host publication	Micro-Electro-Mechanical Systems (MEMS)
Publisher	American Society of Mechanical Engineers (ASME)
Pages	235-240
Number of pages	6
ISBN (Electronic)	9780791816387
DOIs	https://doi.org/10.1115/IMECE1999-0273
State	Published - 1999
Event	ASME 1999 International Mechanical Engineering Congress and Exposition, IMECE 1999 - Nashville, United States Duration: Nov 14 1999 → Nov 19 1999

Publication series

Name	ASME International Mechanical Engineering Congress and Exposition, Proceedings (IMECE)
Volume	1999-W

Conference

Conference	ASME 1999 International Mechanical Engineering Congress and Exposition, IMECE 1999
Country/Territory	United States
City	Nashville
Period	11/14/99 → 11/19/99

ASJC Scopus subject areas

Mechanical Engineering

Access to Document

10.1115/IMECE1999-0273

Cite this

Smekal, T., Briscoe, C., Chan, T., Chang, Y., Dobrin, S., Gallagher, S., Groninger, H., Marrero, R., Rhine, D., Yu, H., & Grodzinski, P. (1999). MODELING, DESIGN AND FABRICATION OF A MICRODEVICE FOR E-FIELD CELL LYSIS. In Micro-Electro-Mechanical Systems (MEMS) (pp. 235-240). (ASME International Mechanical Engineering Congress and Exposition, Proceedings (IMECE); Vol. 1999-W). American Society of Mechanical Engineers (ASME). https://doi.org/10.1115/IMECE1999-0273

MODELING, DESIGN AND FABRICATION OF A MICRODEVICE FOR E-FIELD CELL LYSIS. / Smekal, Thomas; Briscoe, Cindy; Chan, Tony et al.
Micro-Electro-Mechanical Systems (MEMS). American Society of Mechanical Engineers (ASME), 1999. p. 235-240 (ASME International Mechanical Engineering Congress and Exposition, Proceedings (IMECE); Vol. 1999-W).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Smekal, T, Briscoe, C, Chan, T, Chang, Y, Dobrin, S, Gallagher, S, Groninger, H, Marrero, R, Rhine, D, Yu, H & Grodzinski, P 1999, MODELING, DESIGN AND FABRICATION OF A MICRODEVICE FOR E-FIELD CELL LYSIS. in Micro-Electro-Mechanical Systems (MEMS). ASME International Mechanical Engineering Congress and Exposition, Proceedings (IMECE), vol. 1999-W, American Society of Mechanical Engineers (ASME), pp. 235-240, ASME 1999 International Mechanical Engineering Congress and Exposition, IMECE 1999, Nashville, United States, 11/14/99. https://doi.org/10.1115/IMECE1999-0273

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abstract = "The development of an electric field cell lysis device for microfluidic applications will be presented. Detailed modeling of the electrode configuration for the optimization of the electric field has been performed. Device design and fabrication has been accomplished using traditional integrated circuit (IC) manufacturing techniques. As a result, murine lymphocytes have been lysed using several electrode configurations. The requisite electric field strength and pulse parameters for cell lysis were determined. The non-specific adsorption of the released DNA to the materials used in the fabrication of the device was observed. Surface passivation of the fabrication materials was effected through the use of silanization compounds.",

author = "Thomas Smekal and Cindy Briscoe and Tony Chan and Yung Chang and Seth Dobrin and Sean Gallagher and Heidi Groninger and Rob Marrero and David Rhine and Huinan Yu and Piotr Grodzinski",

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AU - Dobrin, Seth

AU - Gallagher, Sean

AU - Groninger, Heidi

AU - Marrero, Rob

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AB - The development of an electric field cell lysis device for microfluidic applications will be presented. Detailed modeling of the electrode configuration for the optimization of the electric field has been performed. Device design and fabrication has been accomplished using traditional integrated circuit (IC) manufacturing techniques. As a result, murine lymphocytes have been lysed using several electrode configurations. The requisite electric field strength and pulse parameters for cell lysis were determined. The non-specific adsorption of the released DNA to the materials used in the fabrication of the device was observed. Surface passivation of the fabrication materials was effected through the use of silanization compounds.

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MODELING, DESIGN AND FABRICATION OF A MICRODEVICE FOR E-FIELD CELL LYSIS

Abstract

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