Mitochondrial gene markers for the hybridizing toads Bufo microscaphus and Bufo woodhousii in Arizona

Trip Lamb, Brian Sullivan, Keith Malmos

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Three restriction site markers from the mitochondrial genome are described for the toads Bufo microscaphus and B. woodhousii. Markers were resolved from DNA sequence data representing segments of cytochrome b (cytb) and 16S ribosomal genes. Two diagnostic restriction sites (BsmA I; SfaN I) characterize B. microscaphus for cytb, whereas a third restriction site (Tsp509 I) distinguishes B. woodhousii for 16S. Restriction digests of PCR-amplified cytb and 16S segments provide an economical, nondestructive assay with which to identify mtDNA haplotypes for these toads and their hybrids. The mtDNA markers complement diagnostic allozyme and call characters, offering an additional approach to examine hybridization between the two species in central Arizona.

Original languageEnglish (US)
Pages (from-to)234-237
Number of pages4
JournalCopeia
Issue number1
StatePublished - Feb 1 2000

Fingerprint

Bufo
toad
toads
cytochrome b
cytochrome
genetic markers
gene
mitochondrial DNA
allozyme
allozymes
haplotypes
complement
hybridization
genome
assay
nucleotide sequences
DNA
assays
marker
genes

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

Mitochondrial gene markers for the hybridizing toads Bufo microscaphus and Bufo woodhousii in Arizona. / Lamb, Trip; Sullivan, Brian; Malmos, Keith.

In: Copeia, No. 1, 01.02.2000, p. 234-237.

Research output: Contribution to journalArticle

@article{4270e3a13aa0437c8bbeac77aeb3b86f,
title = "Mitochondrial gene markers for the hybridizing toads Bufo microscaphus and Bufo woodhousii in Arizona",
abstract = "Three restriction site markers from the mitochondrial genome are described for the toads Bufo microscaphus and B. woodhousii. Markers were resolved from DNA sequence data representing segments of cytochrome b (cytb) and 16S ribosomal genes. Two diagnostic restriction sites (BsmA I; SfaN I) characterize B. microscaphus for cytb, whereas a third restriction site (Tsp509 I) distinguishes B. woodhousii for 16S. Restriction digests of PCR-amplified cytb and 16S segments provide an economical, nondestructive assay with which to identify mtDNA haplotypes for these toads and their hybrids. The mtDNA markers complement diagnostic allozyme and call characters, offering an additional approach to examine hybridization between the two species in central Arizona.",
author = "Trip Lamb and Brian Sullivan and Keith Malmos",
year = "2000",
month = "2",
day = "1",
language = "English (US)",
pages = "234--237",
journal = "Copeia",
issn = "0045-8511",
publisher = "American Society of Ichthyologists and Herpetologists",
number = "1",

}

TY - JOUR

T1 - Mitochondrial gene markers for the hybridizing toads Bufo microscaphus and Bufo woodhousii in Arizona

AU - Lamb, Trip

AU - Sullivan, Brian

AU - Malmos, Keith

PY - 2000/2/1

Y1 - 2000/2/1

N2 - Three restriction site markers from the mitochondrial genome are described for the toads Bufo microscaphus and B. woodhousii. Markers were resolved from DNA sequence data representing segments of cytochrome b (cytb) and 16S ribosomal genes. Two diagnostic restriction sites (BsmA I; SfaN I) characterize B. microscaphus for cytb, whereas a third restriction site (Tsp509 I) distinguishes B. woodhousii for 16S. Restriction digests of PCR-amplified cytb and 16S segments provide an economical, nondestructive assay with which to identify mtDNA haplotypes for these toads and their hybrids. The mtDNA markers complement diagnostic allozyme and call characters, offering an additional approach to examine hybridization between the two species in central Arizona.

AB - Three restriction site markers from the mitochondrial genome are described for the toads Bufo microscaphus and B. woodhousii. Markers were resolved from DNA sequence data representing segments of cytochrome b (cytb) and 16S ribosomal genes. Two diagnostic restriction sites (BsmA I; SfaN I) characterize B. microscaphus for cytb, whereas a third restriction site (Tsp509 I) distinguishes B. woodhousii for 16S. Restriction digests of PCR-amplified cytb and 16S segments provide an economical, nondestructive assay with which to identify mtDNA haplotypes for these toads and their hybrids. The mtDNA markers complement diagnostic allozyme and call characters, offering an additional approach to examine hybridization between the two species in central Arizona.

UR - http://www.scopus.com/inward/record.url?scp=0033976106&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033976106&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:0033976106

SP - 234

EP - 237

JO - Copeia

JF - Copeia

SN - 0045-8511

IS - 1

ER -